Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria

Autor: Karthik Sekar, Roberto Rusconi, John T Sauls, Tobias Fuhrer, Elad Noor, Jen Nguyen, Vicente I Fernandez, Marieke F Buffing, Michael Berney, Suckjoon Jun, Roman Stocker, Uwe Sauer

Publikováno v: Molecular Systems Biology, Vol 14, Iss 11, Pp 1-14 (2018)

Abstract In natural environments, microbes are typically non‐dividing and gauge when nutrients permit division. Current models are phenomenological and specific to nutrient‐rich, exponentially growing cells, thus cannot predict the first division

Externí odkaz: https://doaj.org/article/12cfbcdd67a24e8387cbbf577a075f3f

Bistability in a metabolic network underpins the de novo evolution of colony switching in Pseudomonas fluorescens.

Autor: Jenna Gallie, Eric Libby, Frederic Bertels, Philippe Remigi, Christian B Jendresen, Gayle C Ferguson, Nicolas Desprat, Marieke F Buffing, Uwe Sauer, Hubertus J E Beaumont, Jan Martinussen, Mogens Kilstrup, Paul B Rainey

Publikováno v: PLoS Biology, Vol 13, Iss 3, p e1002109 (2015)

Phenotype switching is commonly observed in nature. This prevalence has allowed the elucidation of a number of underlying molecular mechanisms. However, little is known about how phenotypic switches arise and function in their early evolutionary stag

Externí odkaz: https://doaj.org/article/26ffb0eddbab4a81accba9df362617ad

Capacity for instantaneous catabolism of preferred and non-preferred carbon sources in Escherichia coli and Bacillus subtilis

Autor: Marieke F. Buffing, Hannes Link, Uwe Sauer, Dimitris Christodoulou

Publikováno v: Scientific Reports, 8
Scientific Reports
Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)

Making the right choice for nutrient consumption in an ever-changing environment is a key factor for evolutionary success of bacteria. Here we investigate the regulatory mechanisms that enable dynamic adaptation between non-preferred and preferred ca

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4872c55aa5f9045b397ac9d5661d69f
https://doi.org/10.1038/s41598-018-30266-3

Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization

Autor: Suwanee Jansa-Ard, Raffaele Nicastro, Marieke F. Buffing, Jens Nielsen, Stefan Tippmann, José L. Martínez, Sakda Khoomrung

Publikováno v: Analytical Chemistry Research, Vol 6, Iss C, Pp 9-16 (2015)
Khoomrung, S, Martinez Ruiz, J L, Tippmann, S, Jansa-Ard, S, Buffing, M F, Nicastro, R & Nielsen, J 2015, ' Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization ', Analytical Chemistry Research, vol. 6, pp. 9-16 . https://doi.org/10.1016/j.ancr.2015.10.001

We present an improved extraction and derivatization protocol for GC-MS analysis of amino/non-amino acids in Saccharomyces cerevisiae. Yeast cells were extracted with chloroform: aqueous-methanol (1:1, v/v) and the resulting non-polar and polar extra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d3cef950a4b8984edc71eadf511dbcc
https://doi.org/10.1016/j.ancr.2015.10.001

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