Zobrazeno 1 - 10
of 52
pro vyhledávání: '"Ezequiel Franco-Lara"'
Publikováno v:
Engineering in Life Sciences. 13:399-407
Halophile microorganisms are important ectoine producers and are applied in industrial applications. The growth and osmoregulation are distinguished based on the diversity of environmental distribution and require holistic investigations of cultivati
Autor:
Ulrich Krühne, Eva Maria Akke Palmqvist, Ezequiel Franco-Lara, Andreas Schmid, Frank Schubert, Marc Luebberstedt, Carl-Fredrik Mandenius, Uwe Marx, Frank Kensy, Frank Baganz, Krist V. Gernaey
Publikováno v:
Biotechnology Journal. 7:1308-1314
This perspective article is based on an expert panel review on microbioreactor applications in biochemical and biomedical engineering that was organized by the M³C (measurement, monitoring, modelling and control) Working Group of the European Sectio
Autor:
Miriam Steinwand, Michael Hust, Ezequiel Franco-Lara, Florian David, Anton Ross, Stefan Dübel, Kathrin Bohle
Publikováno v:
Biotechnology Journal. 6:1516-1531
Bacillus megaterium was used as an alternative high potential microbial production system for the production of antibody fragment D1.3 scFv. The aim of the study was to follow a holistic optimization approach from medium screening in small scale micr
Publikováno v:
Engineering in Life Sciences. 11:338-349
Bacillus megaterium expressing a variant of the green fluorescent protein (GFP) was used to investigate the influence of gradients in dissolved oxygen (DO) and fructose concentration on biomass and product formation. For this purpose, several fed-bat
Autor:
Ezequiel Franco-Lara, Eva Jordan, Svenja Lüders, Stefan Dübel, Florian David, Miriam Steinwand, Michael Hust
Publikováno v:
Applied Microbiology and Biotechnology. 91:81-90
Bacillus megaterium was used for production of the lysozyme-specific recombinant scFv D1.3 antibody fragment. Key process parameters like the temperature and the hydromechanical stress play a very important role for significant product formation duri
Autor:
Sobehir Aliasghar Zadeh, Stefanie Demming, Stephanus Büttgenbach, Jordi Vila-Planas, Astrid Edlich, Andreu Llobera, Ezequiel Franco-Lara, Rolf Radespiel
Publikováno v:
ELECTROPHORESIS
We present the development of microbioreactors (MBRs) based on poly(dimethylsiloxane) (PDMS) segmented waveguides (SWG) for local absorbance measurements. Two different MBRs were studied, either using symmetric or asymmetric SWG (being defined as MBR
Autor:
Dietmar C. Hempel, Bernd Nörtemann, Ellen Schultheis, Ezequiel Franco-Lara, Michael Andreas Bahl
Publikováno v:
Carbohydrate Polymers. 80:1037-1041
The separation and purification of the exopolysaccharide PS-EDIV from the culture broth of Sphingomonas pituitosa DSM 13101 was studied. For this purpose a sequence of separation and concentration operations including centrifugation, ultrafiltration
Autor:
Ezequiel Franco-Lara, Rolf Radespiel, Stefanie Demming, Detlev Rasch, Christian J. Kähler, Shobeir Aliasghar Zadeh, Veronika Magdanz, Rodrigo Segura, Stephanus Büttgenbach, Astrid Edlich, Rainer Krull
Publikováno v:
Biotechnology Progress. 26:1259-1270
A diffusion-based microreactor system operated with a reaction volume of 8 μL is presented and characterized to intensify the process understanding in microscale cultivations. Its potential as screening tool for biological processes is evaluated. Th
Autor:
Rainer Krull, Ezequiel Franco-Lara, Stephan Barcikowski, Stephanus Büttgenbach, Stefanie Demming, Astrid Edlich, Anne Hahn
Publikováno v:
physica status solidi (a). 207:898-903
The mergence of microfluidics and nanocomposite materials and their in situ structuring leads to a higher integration level within microsystems technology. Nanoparticles (Cu and Ag) produced via laser radiation were suspended in Poly(dimethylsiloxane
Autor:
Ellen Schultheis, J. I. Escalante, Bernd Nörtemann, Ezequiel Franco-Lara, Michael A. Dreger, José M. Muñoz-Villegas
Publikováno v:
Biochemical Engineering Journal. 47:116-121
Sphingomonas pituitosa excretes the capsular exopolysaccharide PS-EDIV into the culture broth augmenting considerably its fluid viscosity. Since this change particularly affects key processes like mixing and transport during the microbial production,