Capturing the blue-light activated state of the Phot-LOV1 domain from Chlamydomonas reinhardtii using time-resolved serial synchrotron crystallography

Autor: Guillaume Gotthard, Sandra Mous, Tobias Weinert, Raiza Nara Antonelli Maia, Daniel James, Florian Dworkowski, Dardan Gashi, Antonia Furrer, Dmitry Ozerov, Ezequiel Panepucci, Meitian Wang, Gebhard F. X. Schertler, Joachim Heberle, Joerg Standfuss, Przemyslaw Nogly

Publikováno v: IUCrJ, Vol 11, Iss 5, Pp 792-808 (2024)

Light–oxygen–voltage (LOV) domains are small photosensory flavoprotein modules that allow the conversion of external stimuli (sunlight) into intracellular signals responsible for various cell behaviors (e.g. phototropism and chloroplast relocatio

Externí odkaz: https://doaj.org/article/0487f96a71db49698c8ff3e32bc949bf

Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography

Autor: Isabelle Martiel, Chia-Ying Huang, Pablo Villanueva-Perez, Ezequiel Panepucci, Shibom Basu, Martin Caffrey, Bill Pedrini, Oliver Bunk, Marco Stampanoni, Meitian Wang

Publikováno v: IUCrJ, Vol 7, Iss 6, Pp 1131-1141 (2020)

Serial protein crystallography has emerged as a powerful method of data collection on small crystals from challenging targets, such as membrane proteins. Multiple microcrystals need to be located on large and often flat mounts while exposing them to

Externí odkaz: https://doaj.org/article/f0fc2a49b4e14a08bb736f1b42e25766

Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography

Autor: Meitian Wang, Marco Stampanoni, Isabelle Martiel, Martin Caffrey, Oliver Bunk, Ezequiel Panepucci, Pablo Villanueva-Perez, Bill Pedrini, Chia Ying Huang, Shibom Basu

Publikováno v: IUCrJ, Vol 7, Iss 6, Pp 1131-1141 (2020)
IUCrJ, 7 (6)

Serial protein crystallography has emerged as a powerful method of data collection on small crystals from challenging targets, such as membrane proteins. Multiple microcrystals need to be located on large and often flat mounts while exposing them to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6a3cf79d2b7298b6419680872d64af0
http://scripts.iucr.org/cgi-bin/paper?S2052252520013238

Dynamics and mechanism of a light-driven chloride pump

Autor: Sandra Mous, Guillaume Gotthard, David Ehrenberg, Saumik Sen, Tobias Weinert, Philip J. M. Johnson, Daniel James, Karol Nass, Antonia Furrer, Demet Kekilli, Pikyee Ma, Steffen Brünle, Cecilia Maria Casadei, Isabelle Martiel, Florian Dworkowski, Dardan Gashi, Petr Skopintsev, Maximilian Wranik, Gregor Knopp, Ezequiel Panepucci, Valerie Panneels, Claudio Cirelli, Dmitry Ozerov, Gebhard F. X. Schertler, Meitian Wang, Chris Milne, Joerg Standfuss, Igor Schapiro, Joachim Heberle, Przemyslaw Nogly

Publikováno v: Science, 375 (6583)

Chloride transport by microbial rhodopsins is an essential process for which molecular details such as the mechanisms that convert light energy to drive ion pumping and ensure the unidirectionality of the transport have remained elusive. We combined

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f35f634ac8aece2e2b68abccfcc8e1e
https://pubmed.ncbi.nlm.nih.gov/35597714

SwissFEL: The Swiss X-ray Free Electron Laser

Autor: Christopher J. Milne, Thomas Schietinger, Masamitsu Aiba, Arturo Alarcon, Jürgen Alex, Alexander Anghel, Vladimir Arsov, Carl Beard, Paul Beaud, Simona Bettoni, Markus Bopp, Helge Brands, Manuel Brönnimann, Ingo Brunnenkant, Marco Calvi, Alessandro Citterio, Paolo Craievich, Marta Csatari Divall, Mark Dällenbach, Michael D’Amico, Andreas Dax, Yunpei Deng, Alexander Dietrich, Roberto Dinapoli, Edwin Divall, Sladana Dordevic, Simon Ebner, Christian Erny, Hansrudolf Fitze, Uwe Flechsig, Rolf Follath, Franziska Frei, Florian Gärtner, Romain Ganter, Terence Garvey, Zheqiao Geng, Ishkhan Gorgisyan, Christopher Gough, Andreas Hauff, Christoph P. Hauri, Nicole Hiller, Tadej Humar, Stephan Hunziker, Gerhard Ingold, Rasmus Ischebeck, Markus Janousch, Pavle Juranić, Mario Jurcevic, Maik Kaiser, Babak Kalantari, Roger Kalt, Boris Keil, Christoph Kittel, Gregor Knopp, Waldemar Koprek, Henrik T. Lemke, Thomas Lippuner, Daniel Llorente Sancho, Florian Löhl, Carlos Lopez-Cuenca, Fabian Märki, Fabio Marcellini, Goran Marinkovic, Isabelle Martiel, Ralf Menzel, Aldo Mozzanica, Karol Nass, Gian Luca Orlandi, Cigdem Ozkan Loch, Ezequiel Panepucci, Martin Paraliev, Bruce Patterson, Bill Pedrini, Marco Pedrozzi, Patrick Pollet, Claude Pradervand, Eduard Prat, Peter Radi, Jean-Yves Raguin, Sophie Redford, Jens Rehanek, Julien Réhault, Sven Reiche, Matthias Ringele, Jochen Rittmann, Leonid Rivkin, Albert Romann, Marie Ruat, Christian Ruder, Leonardo Sala, Lionel Schebacher, Thomas Schilcher, Volker Schlott, Thomas Schmidt, Bernd Schmitt, Xintian Shi, Markus Stadler, Lukas Stingelin, Werner Sturzenegger, Jakub Szlachetko, Dhanya Thattil, Daniel M. Treyer, Alexandre Trisorio, Wolfgang Tron, Seraphin Vetter, Carlo Vicario, Didier Voulot, Meitian Wang, Thierry Zamofing, Christof Zellweger, Riccardo Zennaro, Elke Zimoch, Rafael Abela, Luc Patthey, Hans-Heinrich Braun

Publikováno v: Applied Sciences, Vol 7, Iss 7, p 720 (2017)

The SwissFEL X-ray Free Electron Laser (XFEL) facility started construction at the Paul Scherrer Institute (Villigen, Switzerland) in 2013 and will be ready to accept its first users in 2018 on the Aramis hard X-ray branch. In the following sections

Externí odkaz: https://doaj.org/article/a8f7a2a146444f3986aab7f7aec73f09