Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Extrachromosomal array"'
Autor:
Runsheng Li, Vincy Ws Ho, Lu-yan Chan, Qiutao Ding, Yu Bi, Xiaoliang Ren, Dongying Xie, Zhongying Zhao
Publikováno v:
G3 (Bethesda, Md.). 12(12)
C. briggsaeas a companion species forC. eleganshas played an increasingly important role in study of evolution of development, gene regulation and genome. Aided by the isolation of its sister spices, it has recently been established as a model for sp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36db91caa5f0678d4709f3e4f9bac891
https://pubmed.ncbi.nlm.nih.gov/36171682
https://pubmed.ncbi.nlm.nih.gov/36171682
Gene editing in C. elegans using plasmid-based CRISPR reagents requires microinjection of many animals to produce a single edit. Germline silencing of plasmid-borne Cas9 is a major cause of inefficient editing. Here, we present a set of C. elegans st
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::88199e7f55e2c0427c81dcbd9a317c4d
https://doi.org/10.1101/2021.08.03.454883
https://doi.org/10.1101/2021.08.03.454883
Autor:
James D. McGhee, Vinci Au, Tobias Wiesenfahrt, Craig J. Marshall, Dylan M. Parker, Paul E Mains, Erica Li-Leger, Frances Snider, Don Moerman, Stephane Flibotte, Erin Osborne Nishimura, Jingjie Duanmu
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 8, Iss 5, Pp 1425-1437 (2018)
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics
The ELT-2 GATA factor normally functions in differentiation of the C. elegans endoderm, downstream of endoderm specification. We have previously shown that, if ELT-2 is expressed sufficiently early, it is also able to specify the endoderm and to repl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a6472d047b89213cc8e70d4238851fd
http://g3journal.org/lookup/doi/10.1534/g3.118.200079
http://g3journal.org/lookup/doi/10.1534/g3.118.200079
Publikováno v:
PLoS Genetics, Vol 17, Iss 11 (2021)
PLoS Genetics, Vol 17, Iss 11, p e1009755 (2021)
PLoS Genetics
PLoS Genetics, Vol 17, Iss 11, p e1009755 (2021)
PLoS Genetics
Gene editing in C. elegans using plasmid-based CRISPR reagents requires microinjection of many animals to produce a single edit. Germline silencing of plasmid-borne Cas9 is a major cause of inefficient editing. Here, we present a set of C. elegans st
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3c5194fd86d12688baf1ad1116d0423
https://doi.org/10.1371/journal.pgen.1009755
https://doi.org/10.1371/journal.pgen.1009755
Autor:
Eric B. Kmiec, Kerry A. Falgowski
Publikováno v:
Gene. 475:87-93
Gene editing by modified single-stranded oligonucleotides is a strategy aimed at inducing single base changes into the genome, generating a permanent genetic change. The work presented here explores gene editing capabilities in the model organism Cae
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22057985756e9f4f5d55ffb9c06278d8
https://doi.org/10.1016/j.gene.2010.12.014
https://doi.org/10.1016/j.gene.2010.12.014
Autor:
Chris E. Hopkins, M. Wayne Davis, Erik M. Jorgensen, Christian Frøkjær-Jensen, Blake J. Newman, Jason M. Thummel, Søren-Peter Olesen, Morten Grunnet
Publikováno v:
Nature Genetics. 40:1375-1383
At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA. These transgenes are typically overexpressed in som
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f28bafdcf7898ca61b4b50f6622014a6
https://doi.org/10.1038/ng.248
https://doi.org/10.1038/ng.248
Publikováno v:
Proceedings of the National Academy of Sciences. 103:16370-16375
Zinc-finger nucleases are chimeric proteins consisting of engineered zinc-finger DNA-binding motifs attached to an endonuclease domain. These proteins can induce site-specific DNA double-strand breaks in genomic DNA, which are then substrates for cel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dee470ad55ac9397bcf909af19d6b92
https://doi.org/10.1073/pnas.0605633103
https://doi.org/10.1073/pnas.0605633103
Autor:
Takuma Sugi, Yasuko Ohtani
Publikováno v:
Biochemical and biophysical research communications. 450(1)
In the neural circuit functional identities of individual neurons are mainly specified by their differential gene expression patterns. Unveiling functional roles of each neuron requires cell-specific interrogation of neural circuitry in the context o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f84e48170e3a4f9f1116c9f2e6c1b3d8
https://pubmed.ncbi.nlm.nih.gov/24942876
https://pubmed.ncbi.nlm.nih.gov/24942876
Publikováno v:
Journal of Visualized Experiments.
Microinjecting DNA into the cytoplasm of the syncytial gonad of Caenorhabditis elegans is the main technique used to establish transgenic lines that exhibit partial and variable transmission rates of extrachromosomal arrays to the next generation. In
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6565368d5dbbaa8761d3474af2d4c3c6
https://doi.org/10.3791/50773
https://doi.org/10.3791/50773
Publikováno v:
Genetics. 143:1181-1191
We describe a genetic mosaic analysis procedure in which Caenorhabditis elegans mosaics are generated by spontaneous loss of an extrachromosomal array. This technique allows almost any C. elegans gene that can be used in germline transformation exper
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d8c6b29cbadf08a328a6538906b79fe
https://doi.org/10.1093/genetics/143.3.1181
https://doi.org/10.1093/genetics/143.3.1181