Výsledky vyhledávání - "Ewan K S McRae"

Akademický článek

Non-averaged single-molecule tertiary structures reveal RNA self-folding through individual-particle cryo-electron tomography

Autor: Jianfang Liu, Ewan K. S. McRae, Meng Zhang, Cody Geary, Ebbe Sloth Andersen, Gang Ren

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)

Abstract Large-scale and continuous conformational changes in the RNA self-folding process present significant challenges for structural studies, often requiring trade-offs between resolution and observational scope. Here, we utilize individual-parti

Externí odkaz: https://doaj.org/article/14396b658a4e45cfa90e15ceb0329d5e

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RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli-Pepper aptamer FRET pair

Autor: Néstor Sampedro Vallina, Ewan K S McRae, Bente Kring Hansen, Adrien Boussebayle, Ebbe Sloth Andersen

Publikováno v: Sampedro Vallina, N, McRae, E K S, Hansen, B K, Boussebayle, A & Andersen, E S 2023, ' RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli-Pepper aptamer FRET pair ', Nucleic Acids Research, vol. 51, no. 9, pp. 4613-4624 . https://doi.org/10.1093/nar/gkad224
Sampedro Vallina, N, McRae, E K S, Hansen, B K, Boussebayle, A & Andersen, E S 2023, ' RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli-Pepper aptamer FRET pair ', Nucleic Acids Research . https://doi.org/10.1093/nar/gkad224

Cryogenic electron microscopy (cryo-EM) is a promising method for characterizing the structure of larger RNA structures and complexes. However, the structure of individual aptamers is difficult to solve by cryo-EM due to their low molecular weight an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c2a22c125f5e7bd61d4b18b2a50edc1
https://pure.au.dk/portal/da/publications/rna-origami-scaffolds-facilitate-cryoem-characterization-of-a-broccolipepper-aptamer-fret-pair(d8f53ecb-3a05-4f1f-ad94-3c644bceb46c).html

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Structure, folding and flexibility of co-transcriptional RNA origami

Autor: Ewan K. S. McRae, Helena Østergaard Rasmussen, Jianfang Liu, Andreas Bøggild, Michael T. A. Nguyen, Nestor Sampedro Vallina, Thomas Boesen, Jan Skov Pedersen, Gang Ren, Cody Geary, Ebbe Sloth Andersen

Publikováno v: Nature nanotechnology, vol 18, iss 7
McRae, E K S, Rasmussen, H Ø, Liu, J, Bøggild, A, Nguyen, M T A, Sampedro Vallina, N, Boesen, T, Pedersen, J S, Ren, G, Geary, C & Andersen, E S 2023, ' Structure, folding and flexibility of co-transcriptional RNA origami ', Nature Nanotechnology . https://doi.org/10.1038/s41565-023-01321-6

RNA origami is a method for designing RNA nanostructures that can self-assemble through co-transcriptional folding with applications in nanomedicine and synthetic biology. However, to advance the method further, an improved understanding of RNA struc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db776adc6c7ddd32d8dda4942734fbc1
https://doi.org/10.1038/s41565-023-01321-6

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Akademický článek

On Characterizing the Interactions between Proteins and Guanine Quadruplex Structures of Nucleic Acids

Autor: Ewan K. S. McRae, Evan P. Booy, Gay Pauline Padilla-Meier, Sean A. McKenna

Publikováno v: Journal of Nucleic Acids, Vol 2017 (2017)

Guanine quadruplexes (G4s) are four-stranded secondary structures of nucleic acids which are stabilized by noncanonical hydrogen bonding systems between the nitrogenous bases as well as extensive base stacking, or pi-pi, interactions. Formation of th

Externí odkaz: https://doaj.org/article/662a9fbabf7b4efc8bedde280078f228

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Monitoring Enzymatic RNA G-Quadruplex Unwinding Activities by Nuclease Sensitivity and Reverse Transcription Stop Assays

Autor: Ewan K S, McRae, Steven J, Dupas, Negar, Atefi, Sean A, McKenna

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2209

Multiple different methods have been employed to investigate the unwinding of RNA G-quadruplexes by various helicase proteins. Each has their own pitfalls, namely, looking at non-native or chemically modified RNA sequences, biasing the unwinding proc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a66ccfaa5eb33f216a42645dfcab368f
https://pubmed.ncbi.nlm.nih.gov/33201469

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2D Saturation Transfer Difference NMR for Determination of Protein Binding Sites on RNA Guanine Quadruplexes

Autor: Ewan K S, McRae, David E, Davidson, Sean A, McKenna

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2161

Saturation transfer difference (STD) NMR is a technique that provides information on the intermolecular interfaces of heterogenous complexes by cross-saturation from one molecule to the other. In this case, selective saturation of protein protons is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::327a1e32ea0f10204eccc6ea577dca1e
https://pubmed.ncbi.nlm.nih.gov/32681509

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Biochemical characterization of G4 quadruplex telomerase RNA unwinding by the RNA helicase RHAU

Autor: Evan P, Booy, Ewan K S, McRae, Sean A, McKenna

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1259

G4 quadruplexes are stable secondary structures prevalent in DNA and RNA that exhibit diverse regulatory functions. Herein, we describe an in vitro technique using the purified RNA helicase RHAU to unwind a G4 quadruplex identified near the 5' end of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::77412b9cccfe22b431889de1745b092e
https://pubmed.ncbi.nlm.nih.gov/25579584

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