Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Ewald T. J. van den Bremer"'
Autor:
Burt G van der Boom, Patrick Van Berkel, Patrick J. Engelberts, Paul W. H. I. Parren, Ronald P. Taylor, Marleen Voorhorst, Margaret A. Lindorfer, Rob N. de Jong, Frank J. Beurskens, Ewald T. J. van den Bremer, Erika M. Cook
Publikováno v:
mAbs
mAbs, 7(4), 672-680
van den Bremer, E T J, Beurskens, F J, Voorhorst, M, Engelberts, P J, de Jong, R N, van der Boom, B G, Cook, E M, Lindorfer, M A, Taylor, R P, van Berkel, P H C & Parren, P W H I 2015, ' Human IgG is produced in a pro-form that requires clipping of C-terminal lysines for maximal complement activation ', mAbs, vol. 7, no. 4, pp. 672-680 . https://doi.org/10.1080/19420862.2015.1046665
mAbs, 7(4), 672-680
van den Bremer, E T J, Beurskens, F J, Voorhorst, M, Engelberts, P J, de Jong, R N, van der Boom, B G, Cook, E M, Lindorfer, M A, Taylor, R P, van Berkel, P H C & Parren, P W H I 2015, ' Human IgG is produced in a pro-form that requires clipping of C-terminal lysines for maximal complement activation ', mAbs, vol. 7, no. 4, pp. 672-680 . https://doi.org/10.1080/19420862.2015.1046665
Human IgG is produced with C-terminal lysines that are cleaved off in circulation. The function of this modification was unknown and generally thought not to affect antibody function. We recently reported that efficient C1q binding and complement-dep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db6e118b7ffe08a83e07dcf101455ebe
https://doi.org/10.1080/19420862.2015.1046665
https://doi.org/10.1080/19420862.2015.1046665
Autor:
Eric Etter, Paul W. H. I. Parren, Esther H Noordergraaf, Justin Long, Michael Gramer, Peter Kopfmann, Tom Lannom, Amitava Kundu, Patrick Van Berkel, Aran F. Labrijn, Janine Schuurman, Muriel D. van Kampen, David Stinehelfer, Ewald T. J. van den Bremer, Jolanda Gerritsen
Publikováno v:
mAbs. 5:962-973
The manufacturing of bispecific antibodies can be challenging for a variety of reasons. For example, protein expression problems, stability issues, or the use of non-standard approaches for manufacturing can result in poor yield or poor facility fit.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b229d2aecc543d05ff5ffd4623f11084
https://doi.org/10.4161/mabs.26233
https://doi.org/10.4161/mabs.26233
Mass spectrometric and calorimetric data reveal that phosphate ion binding to the active site of colicin E9 DNase is delicately regulated by concomitant binding of specific transition metal ions.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::183f942664b0ce5717cf1b9fa3f16244
https://ora.ox.ac.uk/objects/uuid:a920cd21-aee7-4966-a8c7-b18e69da2307
https://ora.ox.ac.uk/objects/uuid:a920cd21-aee7-4966-a8c7-b18e69da2307
Autor:
Piet Gros, Rob N. de Jong, Janine Schuurman, Paul W. H. I. Parren, Ewald T. J. van den Bremer, Albert J. R. Heck, Deniz Ugurlar, Aran F. Labrijn, Frank J. Beurskens, Guanbo Wang
Publikováno v:
Molecular cell. 63(1)
The classical complement pathway contributes to the natural immune defense against pathogens and tumors. IgG antibodies can assemble at the cell surface into hexamers via Fc:Fc interactions, which recruit complement component C1q and induce complemen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49135aee37d488b68d2e7bdd0f9122cd
https://pubmed.ncbi.nlm.nih.gov/27320199
https://pubmed.ncbi.nlm.nih.gov/27320199
Autor:
Stefan Loverix, Ignace Lasters, Janine Schuurman, Rebecca J. Rose, Aran F. Labrijn, Albert J. R. Heck, Paul W. H. I. Parren, Patrick Van Berkel, Ewald T. J. van den Bremer, Jan G. J. van de Winkel
Publikováno v:
Structure, 19(9), 1274-1282. CELL PRESS
Native mass spectrometry (MS) is a powerful technique for studying noncovalent protein-protein interactions. Here, native MS was employed to examine the noncovalent interactions involved in homodimerization of antibody half molecules (HL) in hinge-de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7390d4315c6757b314b2afe7723fe31
https://doi.org/10.1016/j.str.2011.06.016
https://doi.org/10.1016/j.str.2011.06.016
Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose
Autor:
Joseph Brown, Jackie J. Eckblad, Patrick Priem, Jolanda Gerritsen, Michael Gramer, Carrie Shultz, Kent Vickerman, Ewald T. J. van den Bremer, Patrick Van Berkel, Ruth Donahue
Publikováno v:
Biotechnology and Bioengineering. 108:1591-1602
Through process transfer and optimization for increased antibody production to 3 g/L for a GS-CHO cell line, an undesirable drop in antibody Fc galactosylation was observed. Uridine (U), manganese chloride (M), and galactose (G), constituents involve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::843196d8fe94ca2cf92b9a05ec7df625
https://doi.org/10.1002/bit.23075
https://doi.org/10.1002/bit.23075
Autor:
Paul W. H. I. Parren, Marleen Voorhorst, Aran F. Labrijn, Guanbo Wang, Rob N. de Jong, Albert J. R. Heck, Ewald T. J. van den Bremer, Frank J. Beurskens, Janine Schuurman
Publikováno v:
Immunobiology. 221:1143-1144
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8ad376ad9eb0e14c877a10915c23f7ab
https://doi.org/10.1016/j.imbio.2016.06.044
https://doi.org/10.1016/j.imbio.2016.06.044
Autor:
Ewald T. J. van den Bremer, Rob N. de Jong, Janine Schuurman, Arnout F. Gerritsen, Paul W. H. I. Parren, Joyce I. Meesters, Aran F. Labrijn, Muriel D. van Kampen, Patrick Priem
Publikováno v:
Nature protocols. 9(10)
Labrijn et al. describe the generation of bispecific antibodies through controlled Fab-arm exchange, involving separate expression of two parental IgG1s containing single matching point mutations, followed by recombination of the 'half molecules'. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5af1dee95da29ec0c894511a4a1866f0
https://pubmed.ncbi.nlm.nih.gov/25255089
https://pubmed.ncbi.nlm.nih.gov/25255089
Autor:
Paul W. H. I. Parren, Michael Gramer, Ewald T. J. van den Bremer, Jan G. J. van de Winkel, Joost J. Neijssen, Janine Schuurman, Amitava Kundu, Bart De Goeij, Patrick Van Berkel, Kristin Strumane, Muriel D. van Kampen, Joyce I. Meesters, Aran F. Labrijn, Sandra Verploegen
The promise of bispecific antibodies (bsAbs) to yield more effective therapeutics is well recognized; however, the generation of bsAbs in a practical and cost-effective manner has been a formidable challenge. Here we present a technology for the effi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3abb3e3083645e6295d5b3bbc037f617
https://europepmc.org/articles/PMC3612680/
https://europepmc.org/articles/PMC3612680/
Autor:
Stefan Loverix, Ignace Lasters, Aran F. Labrijn, Janine Schuurman, Paul W. H. I. Parren, Ewald T. J. van den Bremer, Jan G. J. van de Winkel, Theo Rispens, Tom Vink, Rebecca J. Rose, Albert J. R. Heck, Tamara H. den Bleker, Rob C. Aalberse, Joyce I. Meesters, Joost J. Neijssen
Publikováno v:
Journal of Immunology, 187(6), 3238-3246. AMER ASSOC IMMUNOLOGISTS
Journal of immunology (Baltimore, Md., 187(6), 3238-3246. American Association of Immunologists
Journal of immunology (Baltimore, Md., 187(6), 3238-3246. American Association of Immunologists
A distinctive feature of human IgG4 is its ability to recombine half molecules (H chain and attached L chain) through a dynamic process termed Fab-arm exchange, which results in bispecific Abs. It is becoming evident that the process of Fab-arm excha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::205d293a6454f4bfec1d4fd63e5a872e
https://pubmed.ncbi.nlm.nih.gov/21841137
https://pubmed.ncbi.nlm.nih.gov/21841137