Heterogeneity in the structural dynamics of Sulfolobus solfataricus β-glycosidase revealed by electron paramagnetic resonance and frequency domain fluorometry

Autor: Roberto Nucci, Ferdinando Febbraio, Gertz I. Likhtenshtein, Ettore Bismuto, Evgenia Lozinsky, Alexander I. Shames

Publikováno v: Protein Science. 11:2535-2544

The local and global dynamics of the Sulfolobus solfataricus beta-glycosidase were studied by electron spin resonance and time-resolved fluorescence techniques. For electron paramagnetic resonance (EPR) investigations, the protein was covalently modi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70ddad77795a9e4f07491b58805faa4c
https://doi.org/10.1110/ps.0212802

Mechanism of Reduction of the Nitrite Ion by Cu I Complexes

Autor: Evgenia Lozinsky, Ariela Burg, Haim Cohen, Dan Meyerstein

Publikováno v: European Journal of Inorganic Chemistry. 2004:3675-3680

The reaction of Cu+aq with NO2− was studied. The results are consistent with the mechanism: Cu+aq + NO2− (Cu+NO2−), K = 140 ± 30 M−1; (Cu+NO2−) + H+ Cu2+ + NO + OH−, k = 4.6 × 105M−1s−1. It is proposed that the copper is bound to th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6e17b7e53bf4e00744be4e7797727bb7
https://doi.org/10.1002/ejic.200400255

Effect of albumin on the kinetics of ascorbate oxidation

Autor: Evgenia Lozinsky, Alexander I. Shames, Oshra Saphier, Artem Novoselsky, Dan Meyerstein, Gertz I. Likhtenshtein

Publikováno v: Biochimica et Biophysica Acta (BBA) - General Subjects. 1526:53-60

The fluorescence intensity of the fluorophore in dansyl piperidine-nitroxide is intramolecularly quenched by the nitroxyl fragment. Therefore, the oxidation of ascorbic acid by the fluorophore-nitroxide (FN) probe can be monitored by two independent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce910abad8632241f148fceb043bcd3b
https://doi.org/10.1016/s0304-4165(01)00100-3

EPR spin labeling study of conformational transitions of β-glycosidase from the hyperthermophilic archaeonSulfolobus solfataricus expressed inEscherichia coli

Autor: C. Vaccaro, Ferdinando Febbraio, Mosè Rossi, Alexander I. Shames, Gertz I. Likhtenshtein, Roberto Nucci, Sabato D'Auria, Evgenia Lozinsky

Publikováno v: Applied magnetic resonance 18 (2000): 515–526. doi:10.1007/BF03162298
info:cnr-pdr/source/autori:Shames AI, Nucci R, D'Auria S, Febbraio F, Vaccaro C, Loznisky E, Rossi M, Likhtenshtein GI./titolo:EPR spin labeling study of conformational transitions of beta-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus expressed in Escherichia coli./doi:10.1007%2FBF03162298/rivista:Applied magnetic resonance/anno:2000/pagina_da:515/pagina_a:526/intervallo_pagine:515–526/volume:18

The conformational transitions of thermophilic β-glycosidase fromSulfolobus solfataricus and the mechanism of its thermal and chemical activation were studied by electron paramagnetic resonance (EPR) of nitroxide spin labels immobilized on the prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b89f5afd1b91725c10de572fc3e8a8ed
https://doi.org/10.1007/bf03162298

Detection of nitric oxide from pig trachea by a fluorescence method

Autor: Zvi Priel, Natalya Uzlaner, Alexander I. Shames, Alexandra Masarwa, Gertz I. Likhtenshtein, Evgenia Lozinsky, Vladimir V Martin, Lioubov V Martina, Dan Meyerstein

Publikováno v: Analytical biochemistry. 326(2)

A nitronyl nitroxide radical covalently linked to an organic fluorophore, pyrene, was used to detect nitric oxide (NO) from freshly excited tissues. This approach is based on the phenomenon of the intramolecular fluorescence quenching of the fluoroph

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58d3866f5d37119a30af261e8e392f2d
https://pubmed.ncbi.nlm.nih.gov/15003554