Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Evelien W. M. Kemna"'
Autor:
Rogier M. Schoeman, Wesley T. E. van den Beld, Evelien W. M. Kemna, Floor Wolbers, Jan C. T. Eijkel, Albert van den Berg
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
Abstract We present a microfluidic chip that enables electrofusion of cells in microdroplets, with exchange of nuclear components. It is shown, to our knowledge for the first time, electrofusion of two HL60 cells, inside a microdroplet. This is the c
Externí odkaz:
https://doaj.org/article/3606b79671de4d729848e07db56bd288
Autor:
Jan C.T. Eijkel, Evelien W. M. Kemna, Floor Wolbers, Rogier M. Schoeman, Albert van den Berg, Wesley T. E. van den Beld
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
Scientific reports, 8:3714. Nature Publishing Group
Scientific Reports
Scientific reports, 8:3714. Nature Publishing Group
Scientific Reports
We present a microfluidic chip that enables electrofusion of cells in microdroplets, with exchange of nuclear components. It is shown, to our knowledge for the first time, electrofusion of two HL60 cells, inside a microdroplet. This is the crucial in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::695d2366324c39ce4c6270de219f0bfc
https://doi.org/10.1038/s41598-018-21993-8
https://doi.org/10.1038/s41598-018-21993-8
Publikováno v:
ELECTROPHORESIS. 35:385-392
In this article, we present a microfluidic device capable of successive high-yield single-cell encapsulation in droplets, with additional droplet pairing, fusion, and shrinkage. Deterministic single-cell encapsulation is realized using Dean-coupled i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::56ccee24e28762fc52ef228801d96f3e
https://doi.org/10.1002/elps.201300179
https://doi.org/10.1002/elps.201300179
Autor:
Albert van den Berg, David A. Weitz, Floor Wolbers, Rogier M. Schoeman, Evelien W. M. Kemna, I. Vermes
Publikováno v:
Lab on a chip, 12(16), 2881-2887. Royal Society of Chemistry
Lab on a Chip
Lab on a Chip
In this article high-yield (77%) and high-speed (2700 cells s(-1)) single cell droplet encapsulation is described using a Dean-coupled inertial ordering of cells in a simple curved continuous microchannel. By introducing the Dean force, the particles
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b27f5363968ee5eb0bcd434a315ca6c
https://doi.org/10.1039/c2lc00013j
https://doi.org/10.1039/c2lc00013j
Publikováno v:
ELECTROPHORESIS
Electrophoresis, 32(22), 3138-3146. Wiley-VCH Verlag
Electrophoresis, 32(22), 3138-3146. Wiley-VCH Verlag
This article describes the development and full characterization of a microfluidic chip for electrofusion of human peripheral blood B-cells and mouse myeloma (NS-1) cells to generate hybridomas. The chip consists of an array of 783 traps, with dimens
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a1ed538552ef6506da1e6960684e616
https://doi.org/10.1002/elps.201100227
https://doi.org/10.1002/elps.201100227
Publikováno v:
Electrophoresis. 35(2-3)
In this article, we present a microfluidic device capable of successive high-yield single-cell encapsulation in droplets, with additional droplet pairing, fusion, and shrinkage. Deterministic single-cell encapsulation is realized using Dean-coupled i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::dbb67d42537ae81e5adf629a7335e066
https://pubmed.ncbi.nlm.nih.gov/23856757
https://pubmed.ncbi.nlm.nih.gov/23856757
Publikováno v:
The Analyst
Analyst, 138(16), 4585-4592. Royal Society of Chemistry
Analyst, 138(16), 4585-4592. Royal Society of Chemistry
Today, droplet based microfluidics has become a standard platform for high-throughput single cell experimentation and analysis. However, until now no label-free, integrated single cell detection and discrimination method in droplets is available. We
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afbb6b59b86e3c428abaf87fe4084dbb