Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste

Autor: Evelien M. te Poele, Tim Devlamynck, Manuel Jäger, Gerrit J. Gerwig, Davy Van de Walle, Koen Dewettinck, Anna K. H. Hirsch, Johannis P. Kamerling, Wim Soetaert, Lubbert Dijkhuizen

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)

Abstract Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness. The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-muta

Externí odkaz: https://doaj.org/article/5e79b1d8bf0d4f5a884762ce3f6ab06c

Insights into Broad-Specificity Starch Modification from the Crystal Structure of Limosilactobacillus Reuteri NCC 2613 4,6-α-Glucanotransferase GtfB

Autor: Joana Gangoiti, Tim Börner, Tjaard Pijning, Evelien M Te Poele, Lubbert Dijkhuizen

Publikováno v: Journal of Agricultural and Food Chemistry, 69(44):05657, 13235-13245. AMER CHEMICAL SOC
Journal of agricultural and food chemistry 69(44), 13235-13245 (2021). doi:10.1021/acs.jafc.1c05657

GtfB-type α-glucanotransferase enzymes from glycoside hydrolase family 70 (GH70) convert starch substrates into α-glucans that are of interest as food ingredients with a low glycemic index. Characterization of several GtfBs showed that they differ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::264414b9e2fdd5af5f726eec9857a5f8
https://doi.org/10.1021/acs.jafc.1c05657

Goat Milk Oligosaccharides

Autor: Anastasia Chrysovalantou Chatziioannou, Eric Benjamins, Evelien M Te Poele, Lubbert Dijkhuizen, Alfred Haandrikman, Sander S. van Leeuwen

Publikováno v: Journal of Agricultural and Food Chemistry

Human milk is considered the golden standard in infant nutrition. Free oligosaccharides in human milk provide important health benefits. These oligosaccharides function as prebiotics, immune modulators, and pathogen inhibitors and were found to impro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cbf5d6857975d688ded27ea1a56d06e
https://doi.org/10.1021/acs.jafc.0c03766

GtfC Enzyme of Geobacillus sp. 12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling

Autor: Anastasia Chrysovalantou Chatziioannou, Lizette A A C M Oudhuis, Stevan E van der Hoek, Hans Leemhuis, Evelien M Te Poele, Wouter J Duisterwinkel, Joana Gangoiti, Gerrit J. Gerwig, Lubbert Dijkhuizen

Publikováno v: Journal of Agricultural and Food Chemistry, 69(34), 9859-9868. AMER CHEMICAL SOC

Starch-acting α-glucanotransferase enzymes are of great interest for applications in the food industry. In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), sy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87363f1c7e29c1087394056bd6b894d0
https://research.rug.nl/en/publications/8b90963d-426c-4b04-89bf-a52d092e2345

Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste profile

Autor: Davy Van de Walle, Evelien M Te Poele, Wim Soetaert, Koen Dewettinck, Lubbert Dijkhuizen, Gerrit J. Gerwig, Koen Quataert, Johannis P. Kamerling, Tim Devlamynck

Publikováno v: Food Chemistry, 272, 653-662. ELSEVIER SCI LTD
FOOD CHEMISTRY

The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners. Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5b322655d81167a16237607a3039002
https://research.rug.nl/en/publications/3e0aa455-0ceb-426b-8741-b2b7dd3adf01