Modeling apoptosis resistance in CHO cells with CRISPR‐mediated knockouts of Bak1, Bax, and Bok

Autor: Michael A. MacDonald, Craig Barry, Teddy Groves, Verónica S. Martínez, Peter P. Gray, Kym Baker, Evan Shave, Stephen Mahler, Trent Munro, Esteban Marcellin, Lars K. Nielsen

Publikováno v: MacDonald, M A, Barry, C, Groves, T, Martínez, V S, Gray, P P, Baker, K, Shave, E, Mahler, S, Munro, T, Marcellin, E & Nielsen, L K 2022, ' Modeling apoptosis resistance in CHO cells with CRISPR-mediated knockouts of Bak1, Bax, and Bok ', Biotechnology and Bioengineering, vol. 119, no. 6, pp. 1380-1391 . https://doi.org/10.1002/bit.28062

Chinese hamster ovary (CHO) cells are the primary platform for the production of biopharmaceuticals. To increase yields, many CHO cell lines have been genetically engineered to resist cell death. However, the kinetics that governs cell fate in biorea

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4ea2d29dfe0a37da2c4d3cd25ac225b
https://doi.org/10.1002/bit.28062

Author response for 'Design and optimization of membrane chromatography for monoclonal antibody charge variant separation'

Autor: null Sathish Nadar, null Balaji Somasundaram, null Marcela Charry, null Jagan Billakanti, null Evan Shave, null Kym Baker, null Linda H. L. Lua

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c9bff8dcbdfa75709ab37243c4d51ee4
https://doi.org/10.1002/btpr.3288/v2/response1

Progression of continuous downstream processing of monoclonal antibodies: Current trends and challenges

Autor: Evan Shave, Balaji Somasundaram, Kym Baker, Linda H.L. Lua, Kristina Pleitt

Publikováno v: Biotechnology and Bioengineering. 115:2893-2907

Rapid advances in intensifying upstream processes for biologics production have left downstream processing as a bottleneck in the manufacturing scheme. Biomanufacturers are pursuing continuous downstream process development to increase efficiency and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a56dbfbfec0dfd7002a5c48df92552ee
https://doi.org/10.1002/bit.26812

Evaluation of process simulation as a decisional tool for biopharmaceutical contract development and manufacturing organizations

Autor: Linda H.L. Lua, Bradley Johnson, Balaji Somasundaram, Kristina Pleitt, Evan Shave

Publikováno v: Biochemical Engineering Journal. 150:107252

Reduction in process time due to continuous bioprocessing is appealing to biopharmaceutical contract development and manufacturing organizations (CDMOs) as it translates to an increased number of batches per year. While continuous bioprocessing can b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8c3cd4a081d32906ef0152bc798f48e5
https://doi.org/10.1016/j.bej.2019.107252

Use of a preparative-scale, recirculating isoelectric trapping device for the isolation and enrichment of acidic proteins in bovine serum

Autor: Gyula Vigh, Evan Shave

Publikováno v: Journal of Chromatography A. 1155:237-241

A recirculating, preparative-scale isoelectric trapping device, developed for the binary isoelectric trapping separation of proteins has been used to desalt, isolate and enrich the pI

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4636d52581d3034e5399ac28507c330
https://doi.org/10.1016/j.chroma.2007.01.125