Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Evan J, Olson"'
Autor:
Amir Shahein, Maria López-Malo, Ivan Istomin, Evan J. Olson, Shiyu Cheng, Sebastian J. Maerkl
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
Here the authors quantitatively characterize binding to transcription factor (TF) binding site clusters in vitro, followed by characterizing clusters in synthetic and native gene regulatory systems in yeast. They show low-affinity clusters achieve hi
Externí odkaz:
https://doaj.org/article/e8f7bcc128234561a5866a93d9992693
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Gene expression noise can reduce fitness but analysis is hampered by a scaling relationship between noise and expression level. Here the authors show that gene expression mean and noise can be independently controlled by expressing two copies of a ge
Externí odkaz:
https://doaj.org/article/37132251cd3a4015b9689403fd278277
Publikováno v:
Molecular Systems Biology, Vol 13, Iss 4, Pp 1-13 (2017)
Abstract Optogenetics combines externally applied light signals and genetically engineered photoreceptors to control cellular processes with unmatched precision. Here, we develop a mathematical model of wavelength‐ and intensity‐dependent photoco
Externí odkaz:
https://doaj.org/article/e014e49d70f5460fb2633d38c5216c57
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 120(2)
Cells cope with and adapt to ever-changing environmental conditions. Sophisticated regulatory networks allow cells to adjust to these fluctuating environments. One such archetypal system is the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::36c936ca579161596104945c33ea492d
https://pubmed.ncbi.nlm.nih.gov/36608289
https://pubmed.ncbi.nlm.nih.gov/36608289
Cells cope with and adapt to ever-changing environmental conditions. Sophisticated regulatory networks allow cells to adjust to these fluctuating environments. One such archetypal system is the Saccharomyces cerevisiae Pho regulon. When external inor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5df3a2ab43c738ad93b630ae10bdbbcc
https://doi.org/10.1101/2022.05.26.493535
https://doi.org/10.1101/2022.05.26.493535
Autor:
Amir Shahein, Maria López-Malo, Ivan Istomin, Evan J. Olson, Shiyu Cheng, Sebastian J. Maerkl
Transcription factor binding to a single binding site and its functional consequence in a promoter context are beginning to be relatively well understood. However, binding to clusters of sites has yet to be characterized in depth, and the functional
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::63d92eb4c9a790c0594f16f620f1bd1a
https://doi.org/10.1101/2021.12.17.473130
https://doi.org/10.1101/2021.12.17.473130
Autor:
John T. Sexton, Evan J Olson, Oleg A. Igoshin, Jeffrey J. Tabor, Brian P. Landry, Sebastian M. Castillo-Hair
Publikováno v:
ACS Synthetic Biology. 5:774-780
Flow cytometry is widely used to measure gene expression and other molecular biological processes with single cell resolution via fluorescent probes. Flow cytometers output data in arbitrary units (a.u.) that vary with the probe, instrument, and sett
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa4b759a592ac2708d020bcd3ef19aae
https://doi.org/10.1021/acssynbio.5b00284
https://doi.org/10.1021/acssynbio.5b00284
Publikováno v:
ACS synthetic biology. 7(1)
Optogenetics is a technology wherein researchers combine light and genetically engineered photoreceptors to control biological processes with unrivaled precision. Near-infrared (NIR) wavelengths (700 nm) are desirable optogenetic inputs due to their
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a68cad5e53fbead78b3cc85c31b1967
https://pubmed.ncbi.nlm.nih.gov/29091422
https://pubmed.ncbi.nlm.nih.gov/29091422
Autor:
Evan J Olson, Jeffrey J. Tabor
Publikováno v:
Nature Chemical Biology. 10:502-511
Systems biologists aim to understand how organism-level processes, such as differentiation and multicellular development, are encoded in DNA. Conversely, synthetic biologists aim to program systems-level biological processes, such as engineered tissu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b41395b612ad300b6fd3fe80a82168b5
https://doi.org/10.1038/nchembio.1559
https://doi.org/10.1038/nchembio.1559
In optogenetics, light signals are used to control genetically engineered photoreceptors, and in turn manipulate biological pathways with unmatched precision. Recently, evolved photoreceptors with diverse in vitro-measured wavelength and intensity-de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82b4da91e8ab6c33f6fd7159a0b4cdb1
https://doi.org/10.1101/081430
https://doi.org/10.1101/081430