Sex-specific DNA-replication in the early mammalian embryo

Autor: Jason Alexander Halliwell, Javier Martin-Gonzalez, Adnan Hashim, John Arne Dahl, Eva R. Hoffmann, Mads Lerdrup

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)

Abstract The timing of DNA replication in mammals is crucial for minimizing errors and influenced by genome usage and chromatin states. Replication timing in the newly formed mammalian embryo remains poorly understood. Here, we have investigated repl

Externí odkaz: https://doaj.org/article/a28619d16a8d4704add66bf4ca318364

SureTypeSCR: R package for rapid quality control and genotyping of SNP arrays from single cells [version 1; peer review: 1 approved, 2 approved with reservations]

Autor: Ivan Vogel, Lishan Cai, Lea Jerman-Plesec, Eva R. Hoffmann

Publikováno v: F1000Research, Vol 10 (2021)

Genotyping of single cells using single nucleotide polymorphism arrays is a cost-effective technology that provides good coverage and precision, but requires whole genome amplification (WGA) due to the low amount of genetic material. Since WGA introd

Externí odkaz: https://doaj.org/article/4e99798a40ef45f0a70a9199ab57105c

Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys

Autor: Lihua Dong, Murat Gul, Simone Hildorf, Susanne Elisabeth Pors, Stine Gry Kristensen, Eva R. Hoffmann, Dina Cortes, Jorgen Thorup, Claus Yding Andersen

Publikováno v: International Journal of Molecular Sciences, Vol 20, Iss 21, p 5390 (2019)

Spermatogonial stem cell (SSC) transplantation therapy is a promising strategy to renew spermatogenesis for prepubertal boys whose fertility is compromised. However, propagation of SSCs is required due to a limited number of SSCs in cryopreserved tes

Externí odkaz: https://doaj.org/article/5ebeb6a472184c78b2e85322c10179d0

Cell-free fetal DNA for genetic evaluation in Copenhagen Pregnancy Loss Study (COPL): a prospective cohort study

Autor: Tanja Schlaikjær Hartwig, Louise Ambye, Jennifer R Gruhn, Jesper Friis Petersen, Tine Wrønding, Letizia Amato, Andrew Chi-Ho Chan, Boyang Ji, Maiken Hemme Bro-Jørgensen, Lene Werge, Mette Marie Babiel Schmidt Petersen, Clara Brinkmann, Julie Birch Petersen, Morten Dunø, Iben Bache, Markus J Herrgård, Finn Stener Jørgensen, Eva R Hoffmann, Henriette Svarre Nielsen, Nina la Cour Freiesleben, Finn Stener Jørgensen Jørgensen, Sofie Bliddal, Therese Juhlin Søndergaard, Sisse Rye Ostrowski, Erik Sørensen, Margit Anita Hørup Larsen, Markus J. Herregård, Eva Hoffmann, Jenny Gruhn, Andy Chi Ho Chan, Astrid Marie Kolte, David Westergaard, Unnur þorsteinsdóttir, Kári Stefánsson, Hákon Jónsson, Ólafur þ. Magnússon, Valgerdur Steinthorsdottir, Lone Schmidt, Karsten Kristiansen, Pia Rørbæk Kamstrup, Mette Nyegaard, Maria Christine Krog, Ellen Christine Leth Løkkegaard, Helle Ejdrup Bredkjær, Charlotte Wilken-Jensen

Publikováno v: Schlaikjær Hartwig, T, Ambye, L, Gruhn, J R, Petersen, J F, Wrønding, T, Amato, L, Chi-Ho Chan, A, Ji, B, Bro-Jørgensen, M H, Werge, L, Petersen, M M B S, Brinkmann, C, Petersen, J B, Dunø, M, Bache, I, Herrgård, M J, Jørgensen, F S, Hoffmann, E R, Nielsen, H S, COPL consortium & Nyegaard, M 2023, ' Cell-free fetal DNA for genetic evaluation in Copenhagen Pregnancy Loss Study (COPL) : a prospective cohort study ', Lancet, vol. 401, no. 10378, pp. 762-771 . https://doi.org/10.1016/S0140-6736(22)02610-1

BACKGROUND: One in four pregnancies end in a pregnancy loss. Although the effect on couples is well documented, evidence-based treatments and prediction models are absent. Fetal aneuploidy is associated with a higher chance of a next successful pregn

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a971b9dd4a4677fb8fb9164bebc33e0d
https://doi.org/10.1016/s0140-6736(22)02610-1

CHK1-CDC25A-CDK1 regulate cell cycle progression in early mouse embryos to protect genome integrity

Autor: Knoblochova Lucie, Duricek Tomas, Vaskovicova Michaela, Zorzompokou Chrysoula, Rayova Diana, Ferencova Ivana, Baran Vladimir, Richard M. Schultz, Eva R. Hoffmann, Drutovic David

After fertilization, remodeling of the oocyte and sperm genomes is essential to convert these highly differentiated non-dividing transcriptionally quiescent cells into early cleavage-stage transcriptionally active totipotent blastomeres. This develop

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b12524f13df51024a5e3a42dc2319fb0
https://doi.org/10.1101/2022.11.13.516318