Long-term adaptation ofSaccharomyces cerevisiaeto the burden of recombinant insulin production

Autor: Erik de Hulster, Ana Luisa B. Cruz, Eva Maria Akke Palmqvist, Lisbeth Olsson, Ali Kazemi Seresht, Marit Hebly, Jean-Marc Daran, Jack T. Pronk, Walter M. van Gulik

Publikováno v: Biotechnology and Bioengineering. 110:2749-2763

High-level production of heterologous proteins is likely to impose a metabolic burden on the host cell and can thus affect various aspects of cellular physiology. A data-driven approach was applied to study the secretory production of a human insulin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cf9392fe315765fe03209a3c7830e757
https://doi.org/10.1002/bit.24927

Monitoring and control of microbioreactors: An expert opinion on development needs

Autor: Ulrich Krühne, Eva Maria Akke Palmqvist, Ezequiel Franco-Lara, Andreas Schmid, Frank Schubert, Marc Luebberstedt, Carl-Fredrik Mandenius, Uwe Marx, Frank Kensy, Frank Baganz, Krist V. Gernaey

Publikováno v: Biotechnology Journal. 7:1308-1314

This perspective article is based on an expert panel review on microbioreactor applications in biochemical and biomedical engineering that was organized by the M³C (measurement, monitoring, modelling and control) Working Group of the European Sectio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86c07cfd4f40857590b0eab454c742fe
https://doi.org/10.1002/biot.201200157

Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p

Autor: Martin Hey-Mogensen, Michael Lisby, Jon W. Poulsen, Sara Larsen, Michael L. Nielsen, Jonas T. Treebak, Kathrine B. Sylvestersen, Marianne A. Andersen, Eva Maria Akke Palmqvist, Christian Madsen, Clifford Young, Per B. Jensen

Publikováno v: Madsen, C T, Sylvestersen, K B, Young, C, Larsen, S C, Poulsen, J W, Andersen, M A, Palmqvist, E A, Hey-Mogensen, M, Jensen, P B, Treebak, J T, Lisby, M & Nielsen, M L 2015, ' Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p ', Nature Communications, vol. 6, 7726 . https://doi.org/10.1038/ncomms8726
Nature Communications

The essential vitamin biotin is a covalent and tenaciously attached prosthetic group in several carboxylases that play important roles in the regulation of energy metabolism. Here we describe increased acetyl-CoA levels and mitochondrial hyperacetyla

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74f281da7f753325903b8212c6285ae7
https://curis.ku.dk/portal/da/publications/biotin-starvation-causes-mitochondrial-protein-hyperacetylation-and-partial-rescue-by-the-sirt3like-deacetylase-hst4p(fc335c2d-c319-428d-8e89-1cb0a07f3a3e).html

The impact of phosphate scarcity on pharmaceutical protein production in S. cerevisiae: linking transcriptomic insights to phenotypic responses

Autor: Ali Kazemi Seresht, Lisbeth Olsson, Eva Maria Akke Palmqvist

Publikováno v: Microbial Cell Factories, Vol 10, Iss 1, p 104 (2011)
Microbial Cell Factories

Background The adaptation of unicellular organisms like Saccharomyces cerevisiae to alternating nutrient availability is of great fundamental and applied interest, as understanding how eukaryotic cells respond to variations in their nutrient supply h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::845db307c9296b559838c6c3b0642f14
https://doi.org/10.1186/1475-2859-10-104