Structure and Dynamics of Three Escherichia coli NfsB Nitro-Reductase Mutants Selected for Enhanced Activity with the Cancer Prodrug CB1954

Autor: Martin A. Day, Andrew J. Christofferson, J. L. Ross Anderson, Simon O. Vass, Adam Evans, Peter F. Searle, Scott A. White, Eva I. Hyde

Publikováno v: International Journal of Molecular Sciences, Vol 24, Iss 6, p 5987 (2023)

Escherichia coli NfsB has been studied extensively for its potential for cancer gene therapy by reducing the prodrug CB1954 to a cytotoxic derivative. We have previously made several mutants with enhanced activity for the prodrug and characterised th

Externí odkaz: https://doaj.org/article/a7ab266e6bf841dbbf8f45408f88e291

The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN

Autor: Antonio E Graziano, Martin A Day, Peter F. Searle, Andrew J. Christofferson, J. L. Ross Anderson, David Jarrom, Eva I. Hyde, Scott A. White

Publikováno v: 'Biochemical Journal ', vol: 478, pages: 2601-2617 (2021)
Day, M A, Jarrom, D, Christofferson, A J, Graziano, A E, Anderson, J L R, Searle, P F, Hyde, E I & White, S A 2021, ' The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN ', Biochemical Journal, vol. 478, no. 13, pp. 2601-2617 . https://doi.org/10.1042/BCJ20210160

NfsA is a dimeric flavoprotein that catalyses the reduction in nitroaromatics and quinones by NADPH. This reduction is required for the activity of nitrofuran antibiotics. The crystal structure of free Escherichia coli NfsA and several homologues hav

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7eca0ec6624e12458c36a47eb25f8a54
https://doi.org/10.1042/bcj20210160

The 3D-structure, kinetics and dynamics of the E. coli nitroreductase NfsA with NADP

Autor: Scott A. White, Andrew J. Christofferson, Alastair I. Grainger, Martin A. Day, David Jarrom, Antonio E. Graziano, Peter F. Searle, Eva I. Hyde

Publikováno v: FEBS lettersReferences. 596(18)

Nitroreductases activate nitroaromatic antibiotics and cancer prodrugs to cytotoxic hydroxylamines and reduce quinones to quinols. Using steady-state and stopped-flow kinetics, we show that the Escherichia coli nitroreductase NfsA is 20-50 fold more

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f979dcdbf0eb9b669a15bb17e6bc49df
https://pubmed.ncbi.nlm.nih.gov/35648111

Structure of SPH (self-incompatibility protein homologue) proteins: a widespread family of small, highly stable, secreted proteins

Autor: Karthik V, Rajasekar, Shuangxi, Ji, Rachel J, Coulthard, Jon P, Ride, Gillian L, Reynolds, Peter J, Winn, Michael J, Wheeler, Eva I, Hyde, Lorna J, Smith

Publikováno v: The Biochemical journal. 476(5)

SPH (self-incompatibility protein homologue) proteins are a large family of small, disulfide-bonded, secreted proteins, initially found in the self-incompatibility response in the field poppy (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::29ae24bc664ae25125b5c8b077c58ab1
https://pubmed.ncbi.nlm.nih.gov/30782970

Rationalisation of a mechanism for sensing single point variants in target DNA using anthracene-tagged base discriminating probes

Autor: Dario M. Bassani, Gediminas Jonusauskas, John Wilkie, Jean-Louis H. A. Duprey, Christian Ludwig, Neil Spencer, Joseph S. Vyle, Alison Rodger, James H. R. Tucker, Eva I. Hyde, Zheng-yun Zhao

Publikováno v: Organic and Biomolecular Chemistry
Organic and Biomolecular Chemistry, Royal Society of Chemistry, 2018, 16 (35), pp.6576-6585. ⟨10.1039/c8ob01710g⟩
Duprey, J-L H A, Bassani, D M, Hyde, E I, Jonusauskas, G, Ludwig, C, Rodger, A, Spencer, N, Vyle, J S, Wilkie, J, Zhao, Z-Y & Tucker, J H R 2018, ' Rationalisation of a mechanism for sensing single point variants in target DNA using anthracene-tagged base discriminating probes ', Organic & biomolecular chemistry, vol. 16, no. 35, pp. 6576-6585 . https://doi.org/10.1039/C8OB01710G, https://doi.org/10.1039/c8ob01710g

International audience; The labelling of DNA oligonucleotides with signalling groups that give a unique response to duplex formation depending on the target sequence is a highly effective strategy in the design of DNA-based hybridisation sensors. A k

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddf7b10e74d7ea7fe9ca258418c3a1dd
https://hal.archives-ouvertes.fr/hal-01953231/file/DupreyOBC2018.pdf