Zobrazeno 1 - 10
of 60
pro vyhledávání: '"Eva Gluenz"'
Autor:
Ulrich Dobramysl, Tom Beneke, Jeremy Charles Mottram, Carolina Moura Costa Catta-Preta, Richard J. Wheeler, Eva Gluenz
Publikováno v:
Wellcome Open Research, Vol 7 (2023)
We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untra
Externí odkaz:
https://doaj.org/article/e907f6e0b6b54cefa1d24516e2f5425b
Autor:
Ulrich Dobramysl, Tom Beneke, Jeremy Charles Mottram, Carolina Moura Costa Catta-Preta, Richard Wheeler, Eva Gluenz
Publikováno v:
Wellcome Open Research, Vol 7 (2022)
We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untra
Externí odkaz:
https://doaj.org/article/9baa413bf1a5409da097c9d557de78f5
Autor:
Caroline R. Espada, José Carlos Quilles, Andreia Albuquerque-Wendt, Mario C. Cruz, Tom Beneke, Lucas B. Lorenzon, Eva Gluenz, Angela K. Cruz, Silvia R. B. Uliana
Publikováno v:
Frontiers in Cellular and Infection Microbiology, Vol 11 (2021)
Until 2015, loss-of-function studies to elucidate protein function in Leishmania relied on gene disruption through homologous recombination. Then, the CRISPR/Cas9 revolution reached these protozoan parasites allowing efficient genome editing with one
Externí odkaz:
https://doaj.org/article/1ed30db54fd34d5c921c74416a427932
Autor:
Tom Beneke, François Demay, Edward Hookway, Nicole Ashman, Heather Jeffery, James Smith, Jessica Valli, Tomas Becvar, Jitka Myskova, Tereza Lestinova, Shahaan Shafiq, Jovana Sadlova, Petr Volf, Richard John Wheeler, Eva Gluenz
Publikováno v:
PLoS Pathogens, Vol 15, Iss 6, p e1007828 (2019)
The protozoan parasite Leishmania possesses a single flagellum, which is remodelled during the parasite's life cycle from a long motile flagellum in promastigote forms in the sand fly to a short immotile flagellum in amastigotes residing in mammalian
Externí odkaz:
https://doaj.org/article/9ca55d87fc984bd0871bdf13faa8f1ce
Autor:
Fernanda Cristina Costa, Amanda Fortes Francisco, Shiromani Jayawardhana, Simone Guedes Calderano, Michael D Lewis, Francisco Olmo, Tom Beneke, Eva Gluenz, Jack Sunter, Samuel Dean, John Morrison Kelly, Martin Craig Taylor
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 12, Iss 4, p e0006388 (2018)
BackgroundInfection with Trypanosoma cruzi causes Chagas disease, a major public health problem throughout Latin America. There is no vaccine and the only drugs have severe side effects. Efforts to generate new therapies are hampered by limitations i
Externí odkaz:
https://doaj.org/article/a2fbb09216b04cdfb28b047579497d03
Publikováno v:
Royal Society Open Science, Vol 4, Iss 5 (2017)
Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens, including kinetoplastids. CRISPR technology provides the opportunity to deve
Externí odkaz:
https://doaj.org/article/38beb60601a64a55a082d30635ce164c
Publikováno v:
PLoS Pathogens, Vol 11, Iss 10, p e1005186 (2015)
Leishmania spp. are protozoan parasites that have two principal life cycle stages: the motile promastigote forms that live in the alimentary tract of the sandfly and the amastigote forms, which are adapted to survive and replicate in the harsh condit
Externí odkaz:
https://doaj.org/article/de0bf2df31df4bb69dd64bb608a32d76
Publikováno v:
Open Biology, Vol 5, Iss 1 (2015)
One of the first steps in understanding a protein's function is to determine its localization; however, the methods for localizing proteins in some systems have not kept pace with the developments in other fields, creating a bottleneck in the analysi
Externí odkaz:
https://doaj.org/article/f005e3948a6d476d87fce0b58510c1bd
Publikováno v:
PLoS ONE, Vol 10, Iss 8, p e0134432 (2015)
In a variety of eukaryotes, flagella play important roles both in motility and as sensory organelles that monitor the extracellular environment. In the parasitic protozoan Leishmania mexicana, one glucose transporter isoform, LmxGT1, is targeted sele
Externí odkaz:
https://doaj.org/article/00ce9c711a5742d59c834fb11786f39f
Publikováno v:
PLoS ONE, Vol 8, Iss 11, p e79581 (2013)
Cell shape is one, often overlooked, way in which protozoan parasites have adapted to a variety of host and vector environments and directional transmissions between these environments. Consequently, different parasite life cycle stages have characte
Externí odkaz:
https://doaj.org/article/167a1153d5c445ad97684a1b588980c2