Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.

Autor: David Gonzalez-Perez, Patricia Molina-Espeja, Eva Garcia-Ruiz, Miguel Alcalde

Publikováno v: PLoS ONE, Vol 9, Iss 3, p e90919 (2014)

Approaches that depend on directed evolution require reliable methods to generate DNA diversity so that mutant libraries can focus on specific target regions. We took advantage of the high frequency of homologous DNA recombination in Saccharomyces ce

Externí odkaz: https://doaj.org/article/da8ac0a787a043b6b5c6745ec1e8acb7

Towards oilcane: Engineering hyperaccumulation of triacylglycerol into sugarcane stems

Autor: Stephen P. Long, Eva Garcia-Ruiz, Georgina Sanahuja, Deepak Kumar, John Shanklin, Baskaran Kannan, Saroj Parajuli, Fredy Altpeter, Huimin Zhao, Ratna Karan, Vijay Singh, Hui Liu

Publikováno v: GCB Bioenergy, Vol 12, Iss 7, Pp 476-490 (2020)

Metabolic engineering to divert carbon flux from sucrose to oil in high biomass crop like sugarcane is an emerging strategy to boost lipid yields per hectare for biodiesel production. Sugarcane stems comprise more than 70% of the crops' biomass and c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc61de386545f852f9bd9c13ec397061
https://doi.org/10.1111/gcbb.12684

Stable and functionally diverse versatile peroxidases by computational design directly from sequence

Autor: Miguel Alcalde, Sarel J. Fleishman, Eva Garcia-Ruiz, Jonathan Jacob Weinstein, Vladimir Mindel, Shiran Barber-Zucker

White-rot fungi secrete a repertoire of high-redox potential oxidoreductases to efficiently decompose lignin. Of these enzymes, versatile peroxidases (VPs) are the most promiscuous biocatalysts. VPs are attractive enzymes for research and industrial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::235ccce80d81e3c990ca87cbe5f5db1a
https://doi.org/10.1101/2021.11.25.469886

Directed -in vitro- evolution of Precambrian and extant Rubiscos

Autor: Antonio Ballesteros, Javier Martin-Diaz, Marisa Rodriguez, Jose M. Sanchez-Ruiz, Miguel Alcalde, Spencer M. Whitney, Paloma Santos-Moriano, Eva Garcia-Ruiz, Bernardo J. Gómez-Fernández, Monica Garcia, Valeria A. Risso, Francisco J. Plou, Patricia Gomez de Santos

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Digital.CSIC. Repositorio Institucional del CSIC
instname
Scientific Reports
Digibug. Repositorio Institucional de la Universidad de Granada
Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid
Consejería de Sanidad de la Comunidad de Madrid

Rubisco is an ancient, catalytically conserved yet slow enzyme, which plays a central role in the biosphere’s carbon cycle. The design of Rubiscos to increase agricultural productivity has hitherto relied on the use of in vivo selection systems, pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d25e86256ececc65d96dd18cfbac6b11
http://link.springer.com/article/10.1038/s41598-018-23869-3

Multiplex Genome Engineering for Optimizing Bioproduction in Saccharomyces cerevisiae

Autor: Junbiao Dai, Tianyi Li, Eva Garcia-Ruiz, Sally Jones, Shuangying Jiang, Jamie Auxillos, Yizhi Cai

Publikováno v: Auxillos, J Y, Garcia-Ruiz, E, Jones, S, Li, T, Jiang, S, Dai, J & Cai, Y 2019, ' Multiplex Genome Engineering for Optimizing Bioproduction in Saccharomyces cerevisiae ', Biochemistry, vol. 58, no. 11, pp. 1492-1500 . https://doi.org/10.1021/acs.biochem.8b01086

The field of synthetic biology is already beginning to realize its potential, with a wealth of examples showcasing the successful genetic engineering of microorganisms for the production of valuable compounds. The chassis Saccharomyces cerevisiae has

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d344e7b71ab90e75e16c00ce59712b3
https://pure.manchester.ac.uk/ws/files/87441584/20181204_GenomeEng_revised_nocolour.docx