Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Eui-cheol Jo"'
Publikováno v:
Biotechnology and Bioprocess Engineering. 24:163-175
Lipoprotein(a) is a proatherogenic, prothrombotic lipoprotein that comprises apolipoprotein(a) (apo(a)) tethered to apolipoprotein B-100, which surrounds a low-density lipoprotein (LDL) moiety. Despite its molecular similarity to plasminogen, apo(a)
Autor:
Yo-Kyung Chung, Chihwa Kim, Eui-cheol Jo, Jinwook Seo, Jongmun Sohn, Byoung-Ju Lee, Hyi-Jeong Ji, Jaehyeon Lee
Publikováno v:
Journal of Human Genetics
Hunter syndrome is an X-linked lysosomal storage disease caused by a deficiency in the enzyme iduronate-2-sulfatase (IDS), leading to the accumulation of glycosaminoglycans (GAGs). Two recombinant enzymes, idursulfase and idursulfase beta are current
Publikováno v:
Biotechnology and Bioprocess Engineering. 6:117-127
The high-level expression of a human single chain urokinase-type plasminogen activator (scu-PA) was achieved by employing a methotrexate (MTX)-dependent gene amplification system in Chinese hamster ovary (CHO) cells. By cotransfecting and coamplifyin
Publikováno v:
Biotechnology and Bioengineering. 42:1218-1228
A series of high-density media for mammalian cell culture were developed by step-fortifications of most nutrient components in RPMI-1640 medium. Each medium constituting the series was constructed to meet in vitro cell growth limitations. Four differ
Microcarrier culture of bowes melanoma cells in serum-free medium with Human plasma fraction IV-4+ V
Publikováno v:
Biotechnology and Bioengineering. 38:247-253
Bowes melanoma cells were cultivated successfully in a serum-free medium which was constructed by the concept of maximum retention of proteins from fractionated human plasma having growth stimulatory activities. The cells could be cultivated in the s
Publikováno v:
Hepatology (Baltimore, Md.). 43(5)
Hepatocellular carcinoma (HCC) constitutes more than 90% of all primary liver cancers. HCC is a hypervascular tumor that develops from dedifferentiation of small avascular HCC and is therefore a good target for anti-angiogenic gene therapy. Recent st
Publikováno v:
Biotechnology and bioengineering. 94(3)
Vectors derived from adeno-associated virus type 2 (AAV2) are promising gene delivery vehicles, but it is still challenging to get the large number of recombinant adeno-associated virus (rAAV) particles required for large animal and clinical studies.
Publikováno v:
Biotechnology progress. 20(6)
The MGpUK-5 cell line, transformed with a single-chain urokinase-type plasminogen activator (scu-PA) minigene, generated mRNA transcripts and scu-PA titers corresponding to 65% or 86% of the amount generated before serum-free adaptation, despite sign
Publikováno v:
Applied microbiology and biotechnology. 65(5)
To characterize the changes in cell growth rate and adenovirus vector (AdV) production capability of 293 cells during culture passages, 293 cells obtained at the 31st culture passage from ATCC (293M #31) were maintained as a monolayer culture and 293
Publikováno v:
Journal of virological methods. 111(2)
Adeno-associated virus type 5 (AAV5), which is distinct from the other serotypes of AAV, has attracted considerable interest as a premier gene delivery vector. As do the other serotypes, AAV5 contains its 4.7 kb-sized, single-stranded genome flanked