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pro vyhledávání: '"Eugenio Meza Mora"'
Autor:
Eugenio Meza Mora, Blanca L. Barrón, Alvaro R. Lara, Gheorghe M. Borja, Guillermo Gosset, Octavio T. Ramírez
Publikováno v:
Microbial Cell Factories, Vol 11, Iss 1, p 132 (2012)
Microbial Cell Factories
Microbial Cell Factories
Background Plasmid DNA (pDNA) is a promising molecule for therapeutic applications. pDNA is produced by Escherichia coli in high cell-density cultivations (HCDC) using fed-batch mode. The typical limitations of such cultivations, including metabolic
Autor:
Sylvie Le Borgne, Guillermo Gosset, Octavio T. Ramírez, Alvaro R. Lara, Tania E. Pablos, Eugenio Meza Mora
Publikováno v:
Biotechnology journal. 6(8)
High cell-density cultivations are the preferred system for biomolecules production by Escherichia coli. It has been previously demonstrated that a strain of E. coli with a modified substrate transport system is able to attain high cell densities in
Autor:
Eugenio Meza Mora, Alvaro R. Lara, Tania E. Pablos, René Soto, Guillermo Gosset, Octavio T. Ramírez, Sylvie Le Borgne
Publikováno v:
Journal of biotechnology. 158(4)
Escherichia coli strains VH33 (PTS − GalP + strain displaying a strongly reduced overflow metabolism) and VH34 (additionally lacking the pyruvate kinase A) were evaluated for the production of a plasmid DNA (pDNA) vaccine. The parent (W3110) and mu