Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Eugene, Rha"'
Autor:
Seong Keun Kim, Paul K. Yoon, Soo‐Jung Kim, Seung‐Gyun Woo, Eugene Rha, Hyewon Lee, Soo‐Jin Yeom, Haseong Kim, Dae‐Hee Lee, Seung‐Goo Lee
Publikováno v:
Microbial Biotechnology, Vol 13, Iss 1, Pp 210-221 (2020)
Summary Targeted gene regulation is indispensable for reprogramming a cellular network to modulate a microbial phenotype. Here, we adopted the type II CRISPR interference (CRISPRi) system for simple and efficient regulation of target genes in Pseudom
Externí odkaz:
https://doaj.org/article/14d7bd97068145e9b06c0b1a2e6b8693
Autor:
Nulee Jang, Jiyeong Jeong, Minji Ko, Dong-Uk Song, Georgii Emelianov, Seong Keun Kim, Eugene Rha, Kil Koang Kwon, Haseong Kim, Dae-Hee Lee, Hyewon Lee, Seung-Goo Lee
Publikováno v:
Journal of Agricultural and Food Chemistry. 71:4924-4931
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b7d0ca681a859438119f63678c0e4104
https://doi.org/10.1021/acs.jafc.3c00286
https://doi.org/10.1021/acs.jafc.3c00286
Autor:
Kil Koang Kwon, Haseong Kim, Soo-Jin Yeom, Eugene Rha, Jinju Lee, Hyewon Lee, Dae-Hee Lee, Seung-Goo Lee
Publikováno v:
Frontiers in Molecular Biosciences, Vol 7 (2021)
Genetic circuits have been developed for quantitative measurement of enzyme activity, metabolic engineering of strain development, and dynamic regulation of microbial cells. A genetic circuit consists of several bio-elements, including enzymes and re
Externí odkaz:
https://doaj.org/article/5adf6b174c7d42bbb2080439dff63992
Autor:
Hyewon Lee, Ji In Baek, Su Jin Kim, Kil Koang Kwon, Eugene Rha, Soo-Jin Yeom, Haseong Kim, Dae-Hee Lee, Dong-Myung Kim, Seung-Goo Lee
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Methanotrophs with soluble methane monooxygenase (sMMO) show high potential for various ecological and biotechnological applications. Here, we developed a high throughput method to identify sMMO-producing microbes by integrating droplet microfluidics
Externí odkaz:
https://doaj.org/article/7ae1ad09caf745c58c31ca6498c9f5de
Autor:
Jin-Young Lee, Sung-Hyun Park, So-Hyung Oh, Jin-Ju Lee, Kil Koang Kwon, Su-Jin Kim, Minjeong Choi, Eugene Rha, Hyewon Lee, Dae-Hee Lee, Bong Hyun Sung, Soo-Jin Yeom, Seung-Goo Lee
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Bioconversion of C1 chemicals such as methane and methanol into higher carbon-chain chemicals has been widely studied. Methanol oxidation catalyzed by methanol dehydrogenase (Mdh) is one of the key steps in methanol utilization in bacterial methylotr
Externí odkaz:
https://doaj.org/article/944fe7358eea40e8987ef99424185f10
Autor:
Soo-Jin Yeom, Moonjeong Kim, Kil Koang Kwon, Yaoyao Fu, Eugene Rha, Sung-Hyun Park, Hyewon Lee, Haseong Kim, Dae-Hee Lee, Dong-Myung Kim, Seung-Goo Lee
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
Efficient biosynthesis of lactams is still undesirable due to lacking of suitable enzyme. Here, the authors develop a sensitive transcription factor-based biosensor for high-throughput screening of marine metagenome and find a cyclase that can cycliz
Externí odkaz:
https://doaj.org/article/cb428eca6741429aa58b48b7daf262a2
Autor:
Seong Keun Kim, Haseong Kim, Seung Gyun Woo, Tae Hyun Kim, Eugene Rha, Kil Koang Kwon, Hyewon Lee, Seung-Goo Lee, Dae-Hee Lee
Publikováno v:
Nucleic Acids Research. 50:13155-13171
Antibiotics have been widely used for plasmid-mediated cell engineering. However, continued use of antibiotics increases the metabolic burden, horizontal gene transfer risks, and biomanufacturing costs. There are limited approaches to maintaining mul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::577b635dfd4331aa373ff8a8646e74f1
https://doi.org/10.1093/nar/gkac1104
https://doi.org/10.1093/nar/gkac1104
Autor:
Soo-Jin Yeom, Kil Koang Kwon, Jung-Ung An, Sung Hyun Park, Jin-Young Lee, Eugene Rha, Hyewon Lee, Haseong Kim, Dae-Hee Lee, Seung-Goo Lee
Publikováno v:
Journal of Agricultural and Food Chemistry. 70:1203-1211
Enantiomerically pure d-amino acids are important intermediates as chiral building blocks for peptidomimetics and semisynthetic antibiotics. Here, a transcriptional factor-based screening strategy was used for the rapid screening of d-stereospecific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24208f974e7b41c2abb7d6cdf7d16479
https://doi.org/10.1021/acs.jafc.1c05834
https://doi.org/10.1021/acs.jafc.1c05834
Autor:
Seong-Kun Bak, Wonjae Seong, Eugene Rha, Hyewon Lee, Seong Keun Kim, Kil Koang Kwon, Haseong Kim, Seung-Goo Lee
Publikováno v:
Journal of microbiology and biotechnology. 32(8)
This study presents a novel DNA part characterization technique that increases throughput by combinatorial DNA part assembly, solid plate-based quantitative fluorescence assay for phenotyping, and barcode tagging-based long-read sequencing for genoty
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94028b16092c0eaf6074628322ed3804
https://pubmed.ncbi.nlm.nih.gov/35879270
https://pubmed.ncbi.nlm.nih.gov/35879270
Autor:
Eugene Rha, Hyewon Lee, Haseong Kim, Soo-Jin Yeom, Seung-Goo Lee, Su-Lim Choi, Dae-Hee Lee, Kil Koang Kwon
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
Scientific Reports
Scientific Reports
Genetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ54)-dependent transcription facto
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c183ddab9afb2c4c675a1bfe95805bc
http://link.springer.com/article/10.1038/s41598-020-62892-1
http://link.springer.com/article/10.1038/s41598-020-62892-1