Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types

Autor: Sascha Conic, Dominique Desplancq, László Tora, Etienne Weiss

Publikováno v: Bio-Protocol, Vol 8, Iss 21 (2018)

The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and to

Externí odkaz: https://doaj.org/article/292f5b1ebd194922b1aa0eccb1c08e32

Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120

Autor: Dominique Desplancq, Corinne Bernard, Annie-Paule Sibler, Bruno Kieffer, Laurent Miguet, Noëlle Potier, Alain Van Dorsselaer, Etienne Weiss

Publikováno v: BioTechniques, Vol 39, Iss 3, Pp 405-411 (2005)

The difficulty and expense of preparing protein samples highly enriched in stable isotopes is a bottleneck for structural studies by nuclear magnetic resonance (NMR) spectroscopy. We have developed a new regulatable expression/labeling vector system

Externí odkaz: https://doaj.org/article/0f97f0c61c3c4104af7876da9daeff92

Supplementary Table from Suppression of cervical carcinoma cell growth by intracytoplasmic codelivery of anti-oncoprotein E6 antibody and small interfering RNA

Autor: Etienne Weiss, Guy Zuber, Mustapha Oulad-Abdelghani, Deniz Dalkara, Gabrielle Zeder-Lutz, Annie-Paule Sibler, Jérôme Courtête

Supplementary Table from Suppression of cervical carcinoma cell growth by intracytoplasmic codelivery of anti-oncoprotein E6 antibody and small interfering RNA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f4e6abe1f59792c2b4c62785db6097d
https://doi.org/10.1158/1535-7163.22487376

A Novel Nanobody Precisely Visualizes Phosphorylated Histone H2AX in Living Cancer Cells under Drug-Induced Replication Stress

Autor: Jeremy Ranniger, Pascal Didier, Manuela Chiper, Gabrielle Zeder-Lutz, Christian Massute, Barbara Di Ventura, Mustapha Oulad-Abdelghani, Eric Moeglin, Arnaud Poterszman, Alastair G. McEwen, Pierre Lafaye, Audrey Stoessel, Dominique Desplancq, Etienne Weiss

Publikováno v: Cancers
Cancers, MDPI, 2021, 13 (13), pp.3317. ⟨10.3390/cancers13133317⟩
Cancers, Vol 13, Iss 3317, p 3317 (2021)
Cancers, 2021, 13 (13), pp.3317. ⟨10.3390/cancers13133317⟩
Volume 13
Issue 13

Simple Summary γ-H2AX, a phosphorylated variant of histone H2A, is a widely used biomarker of DNA replication stress. To develop an immunological probe able to detect and track γ-H2AX in live cancer cells, we have isolated single domain antibodies

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fabae759a76ee03fa4818920b3d2e71
https://hal.archives-ouvertes.fr/hal-03453976

Visualization of Endogenous Transcription Factors in Single Cells Using an Antibody Electroporation-Based Imaging Approach

Autor: Etienne Weiss, Alexia Ferrand, Laszlo Tora, Sascha Conic, Nacho Molina, Dominique Desplancq

Publikováno v: Imaging Gene Expression ISBN: 9781493996735
Imaging Gene Expression
Imaging Gene Expression, pp.209-221, 2019, ⟨10.1007/978-1-4939-9674-2_14⟩
Imaging Gene Expression: Methods and Protocols
Imaging Gene Expression: Methods and Protocols, pp.209-221, 2019, ⟨10.1007/978-1-4939-9674-2_14⟩

In this chapter, we describe an antibody electroporation-based imaging approach that allows for precise imaging and quantification of endogenous transcription factor (i.e., RNA Polymerase II) distributions in single cells using 3D structured illumina

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3efacc21214e0e9c18308784fb68214
https://doi.org/10.1007/978-1-4939-9674-2_14