Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Ethylmaleimide/pharmacology"'
Transmembrane helix XII of UapA, the major fungal homolog of the nucleobase-ascorbate transporter (NAT/NCS2) family, has been proposed to contain an aromatic residue acting as a purine-selectivity filter, distinct from the binding site. To analyze th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=od_____10561::c99911764a33768a89329b69e7422ccd
http://olympias.lib.uoi.gr/jspui/handle/123456789/22644
http://olympias.lib.uoi.gr/jspui/handle/123456789/22644
Publikováno v:
Biochemical Journal, Vol. 360, No Pt 3 (2001) pp. 645-649
Green fluorescent protein (GFP) is used extensively as a reporter protein to monitor cellular processes, including intracellular protein trafficking and secretion. In general, this approach depends on GFP acting as a passive reporter protein. However
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::4d4afc3fe5735b770e4e268ced85bd93
https://archive-ouverte.unige.ch/unige:8829
https://archive-ouverte.unige.ch/unige:8829
Publikováno v:
Biochemistry. 39(20)
The sucrose (CscB) permease belongs to the oligosaccharide:H(+) symporter family of the Major Facilitator Superfamily and is homologous to the lactose permease from Escherichia coli. Sucrose transport in cells expressing sucrose permease is completel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1baa948a2e0c921252f384889fb215f
https://pubmed.ncbi.nlm.nih.gov/10821690
https://pubmed.ncbi.nlm.nih.gov/10821690
Autor:
V. Nathan Subramaniam, Line Roy, Ali Fazel, D. James Morré, Wanjin Hong, Christine Lavoie, Rob Hendriks, John J.M. Bergeron, Amélie Pelletier, Jennifer N. Gushue, Jacques Paiement
Publikováno v:
Molecular Biology of the Cell
Transitional endoplasmic reticulum (tER) consists of confluent rough and smooth endoplasmic reticulum (ER) domains. In a cell-free incubation system, low-density microsomes (1.17 g cc− 1) isolated from rat liver homogenates reconstitute tER by Mg2+
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e1dad8ebe36ca53cef96c789ab2774f
https://igi.indrastra.com/items/show/28251
https://igi.indrastra.com/items/show/28251
Using a functional lactose permease mutant devoid of Cys residues (C-less permease), each amino acid residue in transmembrane domain VIII and flanking hydrophilic loops (from Gln 256 to Lys 289) was replaced individually with Cys. Of the 34 single-Cy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::7bca6baaa849ce24895a97110a022223
https://europepmc.org/articles/PMC2143654/
https://europepmc.org/articles/PMC2143654/
Using a functional lactose permease mutant devoid of Cys residues (C-less permease), each amino acid residue in putative transmembrane helix II and flanking hydrophilic loops (from Leu34 to Lys74) was replaced individually with Cys. Of the 41 single-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb24a180ada13b9626d67f21764f9249
http://olympias.lib.uoi.gr/jspui/handle/123456789/22641
http://olympias.lib.uoi.gr/jspui/handle/123456789/22641
Publikováno v:
Journal of Neuroscience, Vol. 17, No 5 (1997) pp. 1625-1632
Voltage-dependent inhibition of high voltage-activated (HVA) calcium currents by G-proteins can be transiently relieved (facilitated) by strong depolarizing prepulses. However, with respect to the physiological significance of facilitation, it remain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2398f69b6b48ad11460d4dcca0ce6ace
https://archive-ouverte.unige.ch/unige:10517
https://archive-ouverte.unige.ch/unige:10517
Autor:
Frillingos, S., Kaback, H. R.
Using a functional lactose permease mutant devoid of Cys residues (C-less permease), each amino acid residue in putative transmembrane helix VI and the flanking hydrophilic loops (residues 164- 211) was replaced individually with Cys. Of the 48 mutan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=od_____10561::7d204787bde4a3685f6e9687a9751de9
http://olympias.lib.uoi.gr/jspui/handle/123456789/22639
http://olympias.lib.uoi.gr/jspui/handle/123456789/22639
By using a lactose permease mutant containing a single Cys residue in place of Val 331 (helix X), conformational changes induced by ligand binding were studied. With right-side-out membrane vesicles containing Val 331-->Cys permease, lactose transpor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74ad8cb0225b3785e75df7584da045ab
https://europepmc.org/articles/PMC2142758/
https://europepmc.org/articles/PMC2142758/
Deletion of putative transmembrane helix III from the lactose permease of Escherichia coli results in complete loss of transport activity. Similarly, replacement of this region en bloc with 23 contiguous Ala, Leu, or Phe residues abolishes active lac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=od_____10561::3df5af7f90915a52417a11aa8d4027a9
http://olympias.lib.uoi.gr/jspui/handle/123456789/22665
http://olympias.lib.uoi.gr/jspui/handle/123456789/22665