Výsledky vyhledávání - "Esther Y C Koh"

Akademický článek

An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells.

Autor: Esther Y C Koh, Steven C L Ho, Mariati, Zhiwei Song, Xuezhi Bi, Muriel Bardor, Yuansheng Yang

Publikováno v: PLoS ONE, Vol 8, Iss 12, p e82100 (2013)

A set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) elements with varying strengths is generated by mutating the translation initiation codons of 10(th), 11(th), and 12(th) AUG to non-AUG triplets. They are able to

Externí odkaz: https://doaj.org/article/32f5b977ccbd4f19ad7f48c227b8aa08

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Toward stable gene expression in CHO cells

Autor: Steven C. L. Ho, Yuansheng Yang, Esther Y. C. Koh, Mariati, Jessna H. M. Yeo

Publikováno v: Bioengineered. 5:340-345

Maintaining high gene expression level during long-term culture is critical when producing therapeutic recombinant proteins using mammalian cells. Transcriptional silencing of promoters, most likely due to epigenetic events such as DNA methylation an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c801fe30d6bf8c352660df2205ddd02a
https://doi.org/10.4161/bioe.32111

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Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells

Autor: Benjamin P. C. Soo, Mariati, Yuansheng Yang, Steven C. L. Ho, Sheng-Hao Chao, Esther Y. C. Koh

Publikováno v: BMC Biotechnology

Background Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter devoid of CpG dinucleotides, Cp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0adf43f216f2fa57e7c621816141bbbf
https://pubmed.ncbi.nlm.nih.gov/27756290

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Insertion of core CpG island element into human CMV promoter for enhancing recombinant protein expression stability in CHO cells

Autor: Esther Y. C. Koh, Yuansheng Yang, Jessna H. M. Yeo, Steven C. L. Ho, Mariati

Publikováno v: Biotechnology progress. 30(3)

The human cytomegalovirus promoter (hCMV) is susceptible to gene silencing in CHO cells, most likely due to epigenetic events, such as DNA methylation and histone modifications. The core CpG island element (IE) from the hamster adenine phosphoribosyl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d9b33bb0e57fc44c522725fd29ae016
https://pubmed.ncbi.nlm.nih.gov/25482237

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Control of IgG LC:HC ratio in stably transfected CHO cells and study of the impact on expression, aggregation, glycosylation and conformational stability

Autor: Zhiwei Song, Steven Hao-Kee Ho, Esther Y. C. Koh, Corrine Wan, Gavin Teo, Yuansheng Yang, Muriel Bardor, Miranda M. C. van Beers, Monika Mueller, Yen Wah Tong

Publikováno v: Journal of Biotechnology
Journal of Biotechnology, Elsevier, 2013, 165 (3-4), pp.157-166. ⟨10.1016/j.jbiotec.2013.03.019⟩

Immunoglobulin G (IgG), the most common class of commercial monoclonal antibodies (mAbs), exists as multimers of two identical light chains (LC) and two identical heavy chains (HC) assembled together by disulfide bridges. Due to the kinetics of mAb a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7540fc08657acd7264d5a290d8824d4f
https://pubmed.ncbi.nlm.nih.gov/23583871

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