Hunting for the elusive target antigen in gestational alloimmune liver disease (GALD).

Autor: Klaus Rieneck, Karen Koefoed Rasmussen, Erwin M Schoof, Frederik Banch Clausen, Henrietta Holze, Thomas Bergholt, Marianne Hørby Jørgensen, Vibeke Brix Christensen, Runar Almaas, Peter Lüttge Jordal, Marie Locard-Paulet, Kasper Runager, Leif Kofoed Nielsen, Balthasar Clemens Schlotmann, Joachim Lütken Weischenfeldt, Lars Juhl Jensen, Morten Hanefeld Dziegiel

Publikováno v: PLoS ONE, Vol 18, Iss 10, p e0286432 (2023)

The prevailing concept is that gestational alloimmune liver disease (GALD) is caused by maternal antibodies targeting a currently unknown antigen on the liver of the fetus. This leads to deposition of complement on the fetal hepatocytes and death of

Externí odkaz: https://doaj.org/article/6c90ac40f7e14cda8962eef73d907e21

Recent advances in the field of single-cell proteomics

Autor: Valdemaras Petrosius, Erwin M. Schoof

Publikováno v: Translational Oncology, Vol 27, Iss , Pp 101556- (2023)

The field of single-cell omics is rapidly progressing. Although DNA and RNA sequencing-based methods have dominated the field to date, global proteome profiling has also entered the main stage. Single-cell proteomics was facilitated by advancements i

Externí odkaz: https://doaj.org/article/27cc4e9753ae47f09f09a778521f5dbf

Quantitative single-cell proteomics as a tool to characterize cellular hierarchies

Autor: Erwin M. Schoof, Benjamin Furtwängler, Nil Üresin, Nicolas Rapin, Simonas Savickas, Coline Gentil, Eric Lechman, Ulrich auf dem Keller, John E. Dick, Bo T. Porse

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)

Single-cell proteomics can provide insights into the molecular basis for cellular heterogeneity. Here, the authors develop a multiplexed single-cell proteomics and computational workflow, and show that their strategy captures the cellular hierarchies

Externí odkaz: https://doaj.org/article/e6eed179838c43b99f45da74c1d34042

Identification of the global miR-130a targetome reveals a role for TBL1XR1 in hematopoietic stem cell self-renewal and t(8;21) AML

Autor: Gabriela Krivdova, Veronique Voisin, Erwin M. Schoof, Sajid A. Marhon, Alex Murison, Jessica L. McLeod, Martino M. Gabra, Andy G.X. Zeng, Stefan Aigner, Brian A. Yee, Alexander A. Shishkin, Eric L. Van Nostrand, Karin G. Hermans, Aaron C. Trotman-Grant, Nathan Mbong, James A. Kennedy, Olga I. Gan, Elvin Wagenblast, Daniel D. De Carvalho, Leonardo Salmena, Mark D. Minden, Gary D. Bader, Gene W. Yeo, John E. Dick, Eric R. Lechman

Publikováno v: Cell Reports, Vol 38, Iss 10, Pp 110481- (2022)

Summary: Gene expression profiling and proteome analysis of normal and malignant hematopoietic stem cells (HSCs) point to shared core stemness properties. However, discordance between mRNA and protein signatures highlights an important role for post-

Externí odkaz: https://doaj.org/article/939447993067409d9b5458011e74bcb6

High Sensitivity Limited Material Proteomics Empowered by Data-Independent Acquisition on Linear Ion Traps

Autor: Teeradon Phlairaharn, Samuel Grégoire, Lukas R. Woltereck, Valdemaras Petrosius, Benjamin Furtwängler, Brian C. Searle, Erwin M. Schoof

Publikováno v: Phlairaharn, T, Grégoire, S, Woltereck, L R, Petrosius, V, Furtwängler, B, Searle, B C & Schoof, E M 2022, ' High Sensitivity Limited Material Proteomics Empowered by Data-Independent Acquisition on Linear Ion Traps ', Journal of Proteome Research, vol. 21, no. 11, pp. 2815-2826 . https://doi.org/10.1021/acs.jproteome.2c00376

In recent years, the concept of cell heterogeneity in biology has gained increasing attention, concomitant with a push towards technologies capable of resolving such biological complexity at the molecular level. For single-cell proteomics using Mass

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab66c8cca304c9cf16b5b0e80e1456fa
https://doi.org/10.1021/acs.jproteome.2c00376