Zobrazeno 1 - 10
of 49
pro vyhledávání: '"Ernst Wehtje"'
Autor:
Dietlind Adlercreutz, Ernst Wehtje
Publikováno v:
Journal of the American Oil Chemists' Society. 81:553-557
The enzymatic synthesis of PC with decanoic acid in the sn-1 and hexanoic acid in the sn-2 position is described. The procedure comprises the following enzymatic steps: (i) treatment of egg yolk with phospholipase A2 (PLA2) to hydrolyze egg yolk PC t
Publikováno v:
Enzyme and Microbial Technology. 31:833-841
The efficiency of different preparations of lipases was evaluated in organic solvents. Lipases from Humicola lanuginosa, Candida rugosa, Rhizomucor miehei and Pseudomonas cepacia were adsorbed onto the surfactant sorbitan monostearate (Span 60) and t
Publikováno v:
Enzyme and Microbial Technology. 30:916-923
The influence of the reaction conditions on the enantioselectivity of reactions catalysed by lipases or hydroxynitrile leases (HNLs) in organic solvents was investigated. The lipases catalysed kinetic resolution of chiral secondary alcohol, or chiral
Publikováno v:
Enzyme and Microbial Technology. 29:527-534
Five different β-glycosidases (Pyrococcus furiosus β-glucosidase, Sulfolobus solfataricus β-galactosidase, Caldocellum saccharolyticum β-glucosidase, almond β-glucosidase and Escherichia coli β-galactosidase) were evaluated as transglycosylatio
Autor:
Ernst Wehtje, Dietlind Adlercreutz
Publikováno v:
Journal of the American Oil Chemists' Society. 78:1007-1011
A simple HPLC method for the simultaneous analysis of phosphatidylcholine (PC), 1-acyl lysophosphatidylcholine (1-acyl LPC), and 2-acyl lysophosphatidylcholine (2-acyl LPC) with refractive index detection is described. The separation of these three c
Autor:
Ernst Wehtje, Patrick Adlercreutz, Silvia Vaz, Ingemar Svensson, Dietlind Adlercreutz, Mattias Persson, Carmen Virto
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 11:173-178
This text provides a brief overview of the principles of enzymatic lipid conversion and some recent advances in the enzymatic conversion of glycerophospholipids and galactolipids. Lipases and phospholipases are used to exchange fatty acids or the pol
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 11:841-850
A model was developed which describes simultaneous reaction and internal diffusion for kinetically controlled, immobilized α-chymotrypsin-catalyzed, oligopeptide synthesis in acetonitrile medium. The model combines the equations that describe the in
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 11:607-612
Purified hydroxynitrile lyase (HNL) from Manihot esculenta was crystallized by the sitting-drop vapour-diffusion method. The bipyramidal crystals formed (10-20 μm) were cross-linked with different amounts of glutaraldehyde and used as biocatalyst fo
Publikováno v:
Biocatalysis and Biotransformation. 19:119-130
The stability of hydroxynitrile lyase from Hevea brasiliensis has been studied in organic solvents. In dry solvents, the enzyme had half-lives in the range 1400-2500 hours. The enzyme half-life was one order of magnitude lower if the medium was water
Publikováno v:
Biotechnology and Bioengineering. 67:319-326
The selectivity of preparations of alpha-chymotrypsin immobilized on Celite or polyamide and carrying out syntheses of di- and tripeptides in acetonitrile medium were studied. The study concerns the effect of mass-transfer limitations on three differ