Locus-Specific Proteomics by TChP: Targeted Chromatin Purification

Autor: Farzin Pourfarzad, Ali Aghajanirefah, Ernie de Boer, Sara Ten Have, Thamar Bryn van Dijk, Sima Kheradmandkia, Ralph Stadhouders, Supat Thongjuea, Eric Soler, Nynke Gillemans, Marieke von Lindern, Jeroen Demmers, Sjaak Philipsen, Frank Grosveld

Publikováno v: Cell Reports, Vol 4, Iss 3, Pp 589-600 (2013)

Here, we show that transcription factors bound to regulatory sequences can be identified by purifying these unique sequences directly from mammalian cells in vivo. Using targeted chromatin purification (TChP), a double-pull-down strategy with a tetra

Externí odkaz: https://doaj.org/article/8d951a963b1d4a22a3d36a6b419105f2

Heavy chain-only antibodies and tetravalent bispecific antibody neutralizing Staphylococcus aureus leukotoxins

Autor: Rien van Haperen, Maher Saleh, Tristan Bourcier, Hendrik Jan Rademaker, Frank Grosveld, Tao Chen, Ernie de Boer, Rick Janssens, Benoît-Joseph Laventie, Audrey Subilia, Gilles Prévost, Dubravka Drabek, Joyce H. G. Lebbink, Luc Marcellin

Publikováno v: Proceedings of the National Academy of Sciences of the United States of Ame, 108(39), 16404-16409. National Academy of Sciences

Panton–Valentine leukocidin (PVL) is a pore-forming toxin associated with current outbreaks of community-associated methicillin-resistant strains and implicated directly in the pathophysiology of Staphylococcus aureus -related diseases. Humanized h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6fc311d560ab4189f46c0164a0f2f53
https://doi.org/10.1073/pnas.1102265108

The genome-wide dynamics of the binding of Ldb1 complexes during erythroid differentiation

Autor: Christine Steinhoff, Mary Stevens, Erikjan Rijkers, Christel E M Kockx, Jan Christian Bryne, Jun Hou, Frank Grosveld, Boris Lenhard, Robert-Jan Palstra, Supat Thongjuea, Wilfred F. J. van IJcken, Ralph Stadhouders, Ernie de Boer, Eric Soler, Charlotte Andrieu-Soler

Publikováno v: Genes & Development, 24(6), 277-289. Cold Spring Harbor Laboratory Press
Genes & Development
Genes and Development, 24(6). Cold Spring Harbor Laboratory Press

One of the complexes formed by the hematopoietic transcription factor Gata1 is a complex with the Ldb1 (LIM domain-binding protein 1) and Tal1 proteins. It is known to be important for the development and differentiation of the erythroid cell lineage

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aebb0841211fbac140d33f02453990f5
https://pure.eur.nl/en/publications/0b350a53-cb25-4f03-b33d-1ed43a4cfbdd

Heterochromatin Effects on the Frequency and Duration of LCR-Mediated Gene Transcription

Autor: An Langeveld, John Strouboulis, Eric Milot, Nikos Yannoutsos, Peter Fraser, Frank Grosveld, Kian Tan-Un, Wilma Vergeer, Mark Wijgerde, Ernie de Boer, Tolleiv Trimborn

Publikováno v: Cell. 87:105-114

Locus control regions (LCRs) are responsible for initiating and maintaining a stable tissue-specific open chromatin structure of a locus. In transgenic mice, LCRs confer high level expression on linked genes independent of position in the mouse genom

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c71dc44e6e8f19f9d6813bfe61930450
https://doi.org/10.1016/s0092-8674(00)81327-6

Super-resolution imaging reveals 3D folding dynamics of the β-globin locus upon gene activation

Autor: Wiggert A. van Cappellen, Frank Grosveld, Tobias Knoch, Mariëtte van de Corput, Adrian Quintanilla, Ernie de Boer, Leanna Ferrand

Publikováno v: Journal of Cell Science.

The chromatin architecture is constantly changing due to cellular processes such as cell proliferation, differentiation and changes in the expression profile such as gene activation or silencing. Unraveling the changes that occur in the chromatin str

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::097c3e856887f251d184f6a2ea9410eb
https://doi.org/10.1242/jcs.108522