Výsledky vyhledávání - "Erin D. Sheets"

Akademický článek

Molecular Brightness Approach for FRET Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept

Autor: Taryn M. Kay, Cody P. Aplin, Rowan Simonet, Julie Beenken, Robert C. Miller, Christin Libal, Arnold J. Boersma, Erin D. Sheets, Ahmed A. Heikal

Publikováno v: Frontiers in Molecular Biosciences, Vol 8 (2021)

In this report, we have developed a simple approach using single-detector fluorescence autocorrelation spectroscopy (FCS) to investigate the Förster resonance energy transfer (FRET) of genetically encoded, freely diffusing crTC2.1 (mTurquoise2.1–l

Externí odkaz: https://doaj.org/article/da97970603ba4d5395260b6c4b033f25

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Fluorescence depolarization dynamics of ionic strength sensors using time-resolved anisotropy

Autor: Robert C. Miller, Arnold J. Boersma, Erin D. Sheets, Cody P. Aplin, Ahmed A. Heikal, Taryn M. Kay

Publikováno v: Biophys J

Eukaryotic cells encompass dynamic and compartmentalized ionic strength, which impacts a myriad of biological functions such as enzyme activities, protein-protein interactions, and catalytic functions. Herein, we investigated the fluorescence depolar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f86bded5f141ec759a961aa92d1b1f45
https://doi.org/10.1016/j.bpj.2021.01.035

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Molecular Brightness Approach for FRET Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept

Autor: Robert C. Miller, Erin D. Sheets, Arnold J. Boersma, Cody P. Aplin, Ahmed A. Heikal, Rowan Simonet, Julie Beenken, Taryn M. Kay, Christin Libal

Publikováno v: Frontiers in Molecular Biosciences
Frontiers in Molecular Biosciences, Vol 8 (2021)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7766c30658aee4c9cce4642e8e2359d9
https://doi.org/10.3389/fmolb.2021.730394

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Comparative studies of the fluorescence spectroscopy and dynamics of mCerulean3 and mTurquoise2.1 as donors in FRET pairing with mCitrine

Autor: Emmanuel Tetteh-Jada, Julie Beenken, Taryn M. Kay, Erin D. Sheets, Arnold J. Boersma, Cody P. Aplin, Ahmed A. Heikal, Chioma Nwachuku

Publikováno v: Ultrafast Nonlinear Imaging and Spectroscopy VIII.

We investigated the donor effects (mCerulean3 versus mTurquoise2.1) on the spectroscopy and dynamics of mCerulean3-linker-mCitrine constructs using integrated fluorescence spectroscopy methods. Here, mCerulean3 (a cyan fluorescent protein) and mCitri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b8bfbd04059f845578b36b7bd6651780
https://doi.org/10.1117/12.2571138

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FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements

Autor: Robert C. Miller, Alessandro Cembran, Erin D. Sheets, Taryn M. Kay, Arnold J. Boersma, Rowan Simonet, Ryan Leighton, Cody P. Aplin, Ahmed A. Heikal, Christin Libal

Publikováno v: The journal of physical chemistry. B. 124(17)

Living cells are complex, crowded, and dynamic and continually respond to environmental and intracellular stimuli. They also have heterogeneous ionic strength with compartmentalized variations in both intracellular concentrations and types of ions. T

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9677b604531b159ea0937dfcced4d1bb
https://pubmed.ncbi.nlm.nih.gov/32871075

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