High-affinity anti-Arc nanobodies provide tools for structural and functional studies

Autor: Sigurbjörn Markússon, Erik I. Hallin, Helene J. Bustad, Arne Raasakka, Ju Xu, Gopinath Muruganandam, Remy Loris, Aurora Martinez, Clive R. Bramham, Petri Kursula

Publikováno v: PLoS ONE, Vol 17, Iss 6 (2022)

Activity-regulated cytoskeleton-associated protein (Arc) is a multidomain protein of retroviral origin with a vital role in the regulation of synaptic plasticity and memory formation in mammals. However, the mechanistic and structural basis of Arc fu

Externí odkaz: https://doaj.org/article/92f7c7178b3445c785ad94231b2c6722

Raptor-Mediated Proteasomal Degradation of Deamidated 4E-BP2 Regulates Postnatal Neuronal Translation and NF-κB Activity

Autor: Stella Kouloulia, Erik I. Hallin, Konstanze Simbriger, Inês S. Amorim, Gilliard Lach, Theoklitos Amvrosiadis, Kleanthi Chalkiadaki, Agniete Kampaite, Vinh Tai Truong, Mehdi Hooshmandi, Seyed Mehdi Jafarnejad, Paul Skehel, Petri Kursula, Arkady Khoutorsky, Christos G. Gkogkas

Publikováno v: Cell Reports, Vol 29, Iss 11, Pp 3620-3635.e7 (2019)

Summary: The translation initiation repressor 4E-BP2 is deamidated in the brain on asparagines N99/N102 during early postnatal brain development. This post-translational modification enhances 4E-BP2 association with Raptor, a central component of mTO

Externí odkaz: https://doaj.org/article/e93e84be921b440888508dbe8ce36c17

Development and Validation of Arc Nanobodies: New Tools for Probing Arc Dynamics and Function

Autor: Yuta Ishizuka, Tadiwos F. Mergiya, Rodolfo Baldinotti, Ju Xu, Erik I. Hallin, Sigurbjörn Markússon, Petri Kursula, Clive R. Bramham

Publikováno v: Neurochemical Research

Activity-regulated cytoskeleton-associated (Arc) protein plays key roles in long-term synaptic plasticity, memory, and cognitive flexibility. However, an integral understanding of Arc mechanisms is lacking. Arc is proposed to function as an interacti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad01d49c08a007cc2acd012fc748278c
https://hdl.handle.net/11250/3001339

Arc self-association and formation of virus-like capsids are mediated by an N-terminal helical coil motif

Autor: Margaret M. Stratton, Erik I. Hallin, Rory O’Connell, Clive R. Bramham, Gopinath Muruganandam, Shreeram Akerkar, James J. Chambers, Yasunori Hayashi, Ian Merski, Petri Kursula, Helene J. Bustad, Remy Loris, Tambudzai Kanhema, Tomohisa Hosokawa, José M. Valpuesta, Marte I. Flydal, Christine Touma, Maria S. Eriksen, Daniela Lascu, Oleksii Nikolaienko, Sverre Grødem, Jorge Cuéllar, Aurora Martinez

Activity-regulated cytoskeleton-associated protein (Arc) is a protein interaction hub with diverse roles in intracellular neuronal signaling, and important functions in neuronal synaptic plasticity, memory, and postnatal cortical development. Arc has

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c62aa94e02e6b1e7bc97caa5f7b99e0e
http://urn.fi/urn:nbn:fi-fe2021070140815

Structural properties and peptide ligand binding of the capsid homology domains of human Arc

Autor: Petri Kursula, Erik I. Hallin, Clive R. Bramham

Publikováno v: Biochemistry and biophysics reports 26, 100975 (2021). doi:10.1016/j.bbrep.2021.100975
Biochemistry and Biophysics Reports, Vol 26, Iss, Pp 100975-(2021)
Biochemistry and Biophysics Reports

Biochemistry and biophysics reports 26, 100975 (2021). doi:10.1016/j.bbrep.2021.100975
The activity-regulated cytoskeleton-associated protein (Arc) is important for synaptic plasticity and the normal function of the brain. Arc interacts with neu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15753d32aaf3315fe74117c670b04527
https://doi.org/10.1101/2020.12.17.423319