Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Erich Hochuli"'
Publikováno v:
Journal of Chromatography A. 595:113-119
A procedure was developed for oriented immobilization of monoclonal antibodies on a solid support. The technique involves the specific oligosaccharide-directed covalent modification of the monoclonal antibody (mAb) with the chelating peptide, Lys-Gly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bc0e36f6afbd9afe7f8d35ccbc3cbdb
https://doi.org/10.1016/0021-9673(92)85151-i
https://doi.org/10.1016/0021-9673(92)85151-i
Autor:
Erich Hochuli
Publikováno v:
Pure and Applied Chemistry. 64:169-184
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::90f3d72598d0aaceb43cf741a219ffec
https://doi.org/10.1351/pac199264010169
https://doi.org/10.1351/pac199264010169
Publikováno v:
The Journal of Antibiotics. 44:164-171
Anantin, a peptide binding to the receptor of the atrial natriuretic factor (ANF) was isolated from a strain of Streptomyces coerulescens. The molecule consists of 17 natural L-amino acids which form a peptidic ring system. It has a MW of 1,871.0. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dafe986d76cfceefdb0dd4baf7db90bc
https://doi.org/10.7164/antibiotics.44.164
https://doi.org/10.7164/antibiotics.44.164
Autor:
Susan Piesecki, Erich Hochuli
Publikováno v:
Biotechnology Techniques. 8
The strong interaction of chelated nickel ions with a hexahistidine peptide was utilized to identify hexahistidine fusion proteins in crude cellular lysates. The technique involves a Ni2+-nitrilotriacetic acid derivative labelled with alkaline phosph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fb774bab28f01ec11664a79368967cee
https://doi.org/10.1007/bf00207631
https://doi.org/10.1007/bf00207631
Publikováno v:
Biotechnology and bioengineering. 42(2)
The strong interaction of hexa-histidine fusion proteins with metal chelate adsorbents was utilized to immobilize β-galactosidase with a hexa-histidine peptide at the N-terminus to the Ni2+-nitrilotriacetic acid adsorbent. The fusion protein was clo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30341a0ed59e1e7c83b6c7ed14b8334c
https://pubmed.ncbi.nlm.nih.gov/18612978
https://pubmed.ncbi.nlm.nih.gov/18612978
Autor:
Erich Hochuli
Publikováno v:
Modern Methods in Protein-and Nucleic Acid Research
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::33e3ce3c97903231de40d12e4df49a36
https://doi.org/10.1515/9783110853537-007
https://doi.org/10.1515/9783110853537-007
Autor:
Erich Hochuli
Publikováno v:
Genetic Engineering ISBN: 9781461279051
In 1975 Porath and co-workers introduced immobilized metal ion affinity chromatography (IMAC) for the purification of peptides and proteins (1). The principle of this technique is the coordination between the electron donor groups on a protein (pepti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::edbe937cad5a6973f93617541e1858a1
https://doi.org/10.1007/978-1-4613-0641-2_6
https://doi.org/10.1007/978-1-4613-0641-2_6
Autor:
Erich Hochuli
Publikováno v:
Helvetica Chimica Acta. 66:489-493
A Rapid Purification of D-Oxynitrilase from Almond Meal by Affinity Chromatography Oxynitrilase from almond meal is capable of catalyzing the stereospecific addition of cyanide to a variety of aldehydes. Thus, the enzyme is potentially useful in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8470cf8a959e9a7adaad134a7ecc803a
https://doi.org/10.1002/hlca.19830660210
https://doi.org/10.1002/hlca.19830660210
Publikováno v:
Journal of Chromatography A. 411:371-378
After immunoaffinity chromatography, interferon alpha-2a synthesized in bacteria is not homogeneous. Beside oligomers and monomers, a fragment of molecular weight 15,000 was observed. Amino acid analysis and the determination of the amino terminal am
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22950126651d9c7a4d5418c88538750a
https://doi.org/10.1016/s0021-9673(00)93988-8
https://doi.org/10.1016/s0021-9673(00)93988-8