Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Erica B. Fuchs"'
Publikováno v:
Microbial Biotechnology. 1:523-531
Summary Using directed evolution and site-directed mutagenesis, we have isolated a highly thermostable variant of Aspergillus niger glucoamylase (GA), designated CR2-1. CR2-1 includes the previously described mutations Asn20Cys and Ala27Cys (forming
Publikováno v:
Starch - Stärke. 58:501-508
Directed evolution was used to improve the thermostability of Aspergillus niger glucoamylase (GA) expressed in Saccharomyces cerevisiae. A starch-plate assay developed to screen GA mutants for thermostability gave results consistent with those of irr
Publikováno v:
Starch - Stärke. 54:385-392
A single mutation, Asp238→Asn (D238N), of Aspergillus awamori glucoamylase (GA) was identified that increases extracellular production of the enzyme in Saccharomyces cerevisiae at 37 °C. The mutant was isolated as a suppressor of Gly396→Ser (G39
Publikováno v:
Carbohydrate Research. 283:141-150
The acid modification of starch granules has been studied in different ratios of mixtures of the following combination of alcohols, methanol and 2-propanol, methanol and ethanol, methanol and 1-butanol, ethanol and 2-propanol, and ethanol and 1-butan
Publikováno v:
Carbohydrate Research. 281:203-218
The acid modifications of potato, amylomaize-7, and waxy maize starches were conducted in methanol, ethanol, 2-propanol, and 1-butanol using a wide range of acid concentrations (0.36 to 5.0%, w/v) and temperatures of 5 to 65 °C. It was found that li