Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Eric T. Lagally"'
Publikováno v:
Biotechnology and Bioengineering. 111:2265-2279
Standard libraries for systematic evolution of ligands by exponential enrichment (SELEX) typically utilize flanking regions that facilitate amplification of aptamers recovered from each selection round. Here, we show that these flanking sequences can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::19acebab0b00c67d66e900065bbc81e5
https://doi.org/10.1002/bit.25294
https://doi.org/10.1002/bit.25294
Publikováno v:
Langmuir. 26:11609-11614
We present a novel bonding technique for poly(dimethylsiloxane) (PDMS)-based devices employing chemical surface modifications at room temperature. PDMS surfaces were functionalized to present primary amine groups, and glass or gold substrates were fu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::766896d4ad15765fae603a1a7b1ab05f
https://doi.org/10.1021/la1012582
https://doi.org/10.1021/la1012582
Autor:
Eric T. Lagally, Richard A. Mathies
Publikováno v:
Journal of Physics D: Applied Physics. 37:R245-R261
With the completion of the Human Genome Project and the ongoing DNA sequencing of the genomes of other animals, bacteria, plants and others, a wealth of new information about the genetic composition of organisms has become available. However, as the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e73e6dc408ab29a61e97c61059410b5e
https://doi.org/10.1088/0022-3727/37/23/r01
https://doi.org/10.1088/0022-3727/37/23/r01
Autor:
Eric T. Lagally, James R. Scherer, Richard A. Mathies, Binh An Diep, Lee W. Riley, George F. Sensabaugh, Robert G. Blazej, Nicholas Toriello, M Ramchandani
Publikováno v:
Analytical Chemistry. 76:3162-3170
An integrated portable genetic analysis microsystem including PCR amplification and capillary electrophoretic (CE) analysis coupled with a compact instrument for electrical control and laser-excited fluorescence detection has been developed. The micr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::628a509a77351b3b7ec8971c93893db4
https://doi.org/10.1021/ac035310p
https://doi.org/10.1021/ac035310p
Publikováno v:
Sensors and Actuators B: Chemical. 89:315-323
Monolithic elastomer membrane valves and diaphragm pumps suitable for large-scale integration into glass microfluidic analysis devices are fabricated and characterized. Valves and pumps are fabricated by sandwiching an elastomer membrane between etch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::94fb1010ee256fec5e22b37510e76306
https://doi.org/10.1016/s0925-4005(02)00468-9
https://doi.org/10.1016/s0925-4005(02)00468-9
Publikováno v:
Biotechnology and bioengineering. 111(11)
Standard libraries for systematic evolution of ligands by exponential enrichment (SELEX) typically utilize flanking regions that facilitate amplification of aptamers recovered from each selection round. Here, we show that these flanking sequences can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c50aa1d36ad9ef358ad49217db264379
https://pubmed.ncbi.nlm.nih.gov/24895227
https://pubmed.ncbi.nlm.nih.gov/24895227
Publikováno v:
Analytical Chemistry. 73:565-570
Stochastic PCR amplification of single DNA template molecules followed by capillary electrophoretic (CE) analysis of the products is demonstrated in an integrated microfluidic device. The microdevice consists of submicroliter PCR chambers etched into
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fca5d0044f0b234e566c63ab28a14895
https://doi.org/10.1021/ac001026b
https://doi.org/10.1021/ac001026b
Publikováno v:
Sensors and Actuators B: Chemical. 63:138-146
A monolithic integrated DNA analysis system comprised of microfluidic valves and vents, PCR amplification chambers, and capillary electrophoretic separation channels has been microfabricated in a glass sandwich structure. Valves and hydrophobic vents
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3dd04007d4799010956c624797ea22e9
https://doi.org/10.1016/s0925-4005(00)00350-6
https://doi.org/10.1016/s0925-4005(00)00350-6
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 949
The reliable detection of ligand and analyte binding is of significant importance for the field of medical diagnostics. Recent advances in proteomics and the rapid expansion in the number of identified protein biomarkers enhance the need for reliable
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f10829a476a4cc71601f20c9467bc460
https://pubmed.ncbi.nlm.nih.gov/23329461
https://pubmed.ncbi.nlm.nih.gov/23329461
Autor:
Cheng Wei T, Yang, Eric T, Lagally
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 949
Microfluidics has emerged as a versatile technology that has found many applications, including DNA chips, fuel cells, and diagnostics. As the field of microfluidic diagnostics grows, it is important to introduce the principles of this technology to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::09ea21a69843cbd2c24c0a141c5eed84
https://pubmed.ncbi.nlm.nih.gov/23329433
https://pubmed.ncbi.nlm.nih.gov/23329433