Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Eric J. Strobel"'
Autor:
Courtney E. Szyjka, Eric J. Strobel
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-22 (2023)
Abstract RNA begins to fold as it is transcribed by an RNA polymerase. Consequently, RNA folding is constrained by the direction and rate of transcription. Understanding how RNA folds into secondary and tertiary structures therefore requires methods
Externí odkaz:
https://doaj.org/article/fcf4e71f1b934d58862e772cff319c44
Autor:
Courtney E. Szyjka, Eric J. Strobel
Publikováno v:
bioRxiv
RNA begins to fold as it is transcribed by an RNA polymerase. Consequently, RNA folding is constrained by the direction and rate of transcription. Understanding how RNA folds into secondary and tertiary structures therefore requires methods for deter
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::952ea18856995735d52638b94205fc2c
https://europepmc.org/articles/PMC9980086/
https://europepmc.org/articles/PMC9980086/
Autor:
Eric J. Strobel
Publikováno v:
ChemBioChem. 22:3214-3224
Site-specific strategies for exchanging segments of dsDNA are important for DNA library construction and molecular tagging. Deoxyuridine (dU) excision is an approach for generating 3’ ssDNA overhangs in gene assembly and molecular cloning procedure
Publikováno v:
Methods in enzymology. 675
Synchronized transcription elongation complexes (TECs) are a fundamental tool for investigating the biochemical properties of RNA polymerases (RNAPs) and nascent RNA. We recently developed a standardized system for isolating high-purity synchronized
Synchronized transcription elongation complexes (TECs) are a fundamental tool for in vitro studies of transcription and RNA folding. Transcription elongation can be synchronized by omitting one or more NTPs from an in vitro transcription reaction so
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4d08075f5b64b423417f5e1162aef00a
https://doi.org/10.1101/2022.01.10.475675
https://doi.org/10.1101/2022.01.10.475675
Autor:
Courtney E. Szyjka, Eric J. Strobel
Publikováno v:
Riboregulator Design and Analysis ISBN: 9781071624203
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7fb534f8627a2a854cfced780eef438d
https://doi.org/10.1007/978-1-0716-2421-0_17
https://doi.org/10.1007/978-1-0716-2421-0_17
Publikováno v:
Integrated Methods in Protein Biochemistry: Part A ISBN: 9780323992664
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6e5f8f4b6812c4652d3252bd0866a123
https://doi.org/10.1016/bs.mie.2022.07.008
https://doi.org/10.1016/bs.mie.2022.07.008
Publikováno v:
Nature chemical biology
Cotranscriptional folding is an obligate step of RNA biogenesis that can guide RNA structure formation and function through transient intermediate folds. This process is particularly important for transcriptional riboswitches in which the formation o
Autor:
Eric J. Strobel
Publikováno v:
The Journal of Biological Chemistry
In vitro studies of transcription frequently require the preparation of defined elongation complexes. Defined transcription elongation complexes (TECs) are typically prepared by constructing an artificial transcription bubble from synthetic oligonucl