Development of a tetrameric streptavidin mutein with reversible biotin binding capability: engineering a mobile loop as an exit door for biotin.

Autor: Valerie J O'Sullivan, Isabelle Barrette-Ng, Eric Hommema, Greg T Hermanson, Mark Schofield, Sau-Ching Wu, Claudia Honetschlaeger, Kenneth K-S Ng, Sui-Lam Wong

Publikováno v: PLoS ONE, Vol 7, Iss 4, p e35203 (2012)

A novel form of tetrameric streptavidin has been engineered to have reversible biotin binding capability. In wild-type streptavidin, loop(3-4) functions as a lid for the entry and exit of biotin. When biotin is bound, interactions between biotin and

Externí odkaz: https://doaj.org/article/b787c9ced222455f9c7126e5cd1feb32

Development of a Tetrameric Streptavidin Mutein with Reversible Biotin Binding Capability: Engineering a Mobile Loop as an Exit Door for Biotin

Autor: Sui-Lam Wong, Greg T. Hermanson, Sau-Ching Wu, Claudia Honetschlaeger, Valerie J. O'Sullivan, Eric Hommema, Isabelle Barrette-Ng, Mark J. Schofield, Kenneth K.-S. Ng

Publikováno v: PLoS ONE
PLoS ONE, Vol 7, Iss 4, p e35203 (2012)

A novel form of tetrameric streptavidin has been engineered to have reversible biotin binding capability. In wild-type streptavidin, loop(3-4) functions as a lid for the entry and exit of biotin. When biotin is bound, interactions between biotin and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa45b99b3b9014a4cdcd36a228e10eec
http://europepmc.org/articles/PMC3334968

Human In Vitro Translation Systems for Glycoprotein and Phosphoprotein Expression

Autor: Brian Webb, Satoshi Mikami, Gregory John Kilmer, Eric Hommema, Penny Jensen, Hiroaki Imataka

Publikováno v: The FASEB Journal. 25

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::53cd2eb3c3d9a29a552e1e5537291e77
https://doi.org/10.1096/fasebj.25.1_supplement.lb144

A global benchmark study using affinity-based biosensors

Autor: Aykut Üren, Stephen G. Brohawn, Eva Muñoz, Kenneth Miller, Mike Scott, Heather Hughes, Yasmina Noubia Abdiche, Rohn Lee Millican, Anne W Emerick, Jonathan Brooks, Michael B. Murphy, Charlene S. Lee, Carmelo Di Primo, Joshua Ballard, Quyhn Trinh, Giuseppe A. Papalia, Cynthia S. Hinck, Kevin Thompson, Peter Flynn, Joshua S. Klein, Martin A. Wear, Mark Toews, Richard N. Bohnsack, Mohammed Yousef, Daniel Malashock, Gaetano Barbato, Jill Raymond, Alison Joyce, Min Hsiang Yang, Julie Rothacker, Patrick England, Ashique Rafique, Jacinto López-Sagaseta, Scott L. Klakamp, Daulet Satpaev, Monica Dines, Danny Terwey, Islay Campbell, Joshua Thompson, Ben Busby, Tanmoy Ganguly, Gerardo Gutierrez-Sanchez, Sergei Shikov, Kevin Lindquist, Momchilo Vuyisich, Asya Grinberg, Krista Witte, Yun Hee Cho, Yue Ji Li, Hubert Mantz, Ewa Pol, Gonzalo Obal, Brian J. Pak, María J. Hernáiz, Peter Kainz, Erk Gedig, Mary M. Murphy, Tsafir Bravman, Janus Krarup, Gabrielle Zeder-Lutz, John Quinn, Thomas E. Ryan, Pietro Brandani, Dawn Kernaghan, Anca Clabbers, M. Brent Waddell, Agnes Puskas, Petr Skládal, Sanjay Nilapwar, Kara Herlihy, Gregor Anderluh, Federico Torta, Eric Hommema, Andrej Bavdek, Rejane Guimaraes, Anthony M. Giannetti, Eric Fang, Henrik Anderson, Anne Birgitte Bagge Hagel, Schuyler B Corry, Mark R. Witmer, Phillippe Neuner, Sébastien Wieckowski, Nico Dankbar, Alanna Pinkerton, Suparna Mundodo, Trevor D. Chapman, Sylvie Canepa, Satya P. Yadav, Rostislav Skrabana, Oleksandr Kalyuzhniy, Phini S Katsamba, Vidya Chandrasekaran, Eugene G. Chomey, Dana Reichmann, Katy McGirr, Dotzlaf Joe Edward, Mireille Baltzinger, Ite A. Laird-Offringa, Liann Wang, Erica Boni, Tiffany Tsang, Zaneta Nikolovska-Coleska, Andreas Schoenemann, Yuki Abe, Jamie Furneisen, Kenneth T. Lewis, Eileen M. Lafer, John Corbin, Satyen Gautam, Yuguo Feng, Olan Dolezal, Sylviane Hoos, James R. Horn, Bianca Beusink, Jinlin Peng, Otto Pritsch, Kristian H. Schlick, Ryan James Darling, Malgorzata Mikolajczyk, Quincy L. Carter, Jason T. Schuman, Yang Liu, Abdelkrim Khadir, Loïc Martin, Mark Alan Lewis, Ganeshram Krishnamoorthy, Andrew W. Drake, Jason Baardsnes, George Korza, Jesper Pass, Judie Berlier, Melicia Gainey, Nguyen Ly, James R. Partridge, Rosy Calvert, Roberta D'Agata, Rebecca L. Rich, Monica E. Ferreira, Phillip Page, Frank John Podlaski, Jay Duffner, Ruchira Das Gupta, Melanie Wong, Sergei Bibikov, Jiejin Li, Paola Torreri, Bruce A. Andrien, Peter Spies, Christina Boozer, David G. Myszka

Publikováno v: Analytical Biochemistry
Analytical Biochemistry, Elsevier Masson, 2009, 386 (2), pp.194-216. ⟨10.1016/j.ab.2008.11.021⟩
Analytical biochemistry, 386(2):10.1016/j.ab.2008.11.021, 194-216. Academic Press Inc.
Analytical Biochemistry, 2009, 386 (2), pp.194-216. ⟨10.1016/j.ab.2008.11.021⟩
Rich, R L, Papalia, G A, Flynn, P J, Furneisen, J, Quinn, J, Klein, J S, Katsamba, P S, Waddell, M B, Scott, M, Thompson, J, Berlier, J, Corry, S, Baltzinger, M, Zeder-Lutz, G, Schoenemann, A, Clabbers, A, Wieckowski, S, Murphy, M M, Page, P, Ryan, T E, Duffner, J, Ganguly, T, Corbin, J, Gautam, S, Anderluh, G, Bavdek, A, Reichmann, D, Yadav, S P, Hommema, E, Pol, E, Drake, A, Klakamp, S, Chapman, T, Kernaghan, D, Miller, K, Schuman, J, Lindquist, K, Herlihy, K, Murphy, M B, Bohnsack, R, Andrien, B, Brandani, P, Terwey, D, Millican, R, Darling, R J, Wang, L, Carter, Q, Dotzlaf, J, Lopez-Sagaseta, J, Campbell, I, Torreri, P, Hoos, S, England, P, Liu, Y, Abdiche, Y, Malashock, D, Pinkerton, A, Wong, M, Lafer, E, Hinck, C, Thompson, K, Primo, C D, Joyce, A, Brooks, J, Torta, F, Bagge Hagel, A B, Krarup, J, Pass, J, Ferreira, M, Shikov, S, Mikolajczyk, M, Abe, Y, Barbato, G, Giannetti, A M, Krishnamoorthy, G, Beusink, B, Satpaev, D, Tsang, T, Fang, E, Partridge, J, Brohawn, S, Horn, J, Pritsch, O, Obal, G, Nilapwar, S, Busby, B, Gutierrez-Sanchez, G, Gupta, R D, Canepa, S, Witte, K, Nikolovska-Coleska, Z, Cho, Y H, D'Agata, R, Schlick, K, Calvert, R, Munoz, E M, Hernaiz, M J, Bravman, T, Dines, M, Yang, M-H, Puskas, A, Boni, E, Li, J, Wear, M, Grinberg, A, Baardsnes, J, Dolezal, O, Gainey, M, Anderson, H, Peng, J, Lewis, M, Spies, P, Trinh, Q, Bibikov, S, Raymond, J, Yousef, M, Chandrasekaran, V, Feng, Y, Emerick, A, Mundodo, S, Guimaraes, R, McGirr, K, Li, Y-J, Hughes, H, Mantz, H, Skrabana, R, Witmer, M, Ballard, J, Martin, L, Skladal, P, Korza, G, Laird-Offringa, I, Lee, C S, Khadir, A, Podlaski, F, Neuner, P, Rothacker, J, Rafique, A, Dankbar, N, Kainz, P, Gedig, E, Vuyisich, M, Boozer, C, Ly, N, Toews, M, Uren, A, Kalyuzhniy, O, Lewis, K, Chomey, E, Pak, B J & Myszka, D G 2009, ' A global benchmark study using affinity-based biosensors ', Analytical Biochemistry, vol. 386, no. 2, pp. 194-216 . https://doi.org/10.1016/j.ab.2008.11.021

International audience; To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15f0511d7d5e8ed6e3424eec0c582e12
https://hal.archives-ouvertes.fr/hal-01527917

Antibody Microarray Analysis of Inflammatory Mediator Release by Human Leukemia T-Cells and Human Non–Small Cell Lung Cancer Cells

Autor: Bradley Garcia, Aric Morgan, Eric Hommema, Aubrey Hargrave, Rick Wiese, Janaki Narahari, Brian Webb, Greg Kilmer

Cytokines and chemokines are responsible for regulating inflammation and the immune response. Cytokine and chemokine release is typically measured by quantitative enzyme-linked immunosorbant assay (ELISA) or Western blot analysis. To expedite the ana

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78d46620c542a7ffccef26221b054c95
https://europepmc.org/articles/PMC2062556/