Rapid quantification of insulin degludec by immunopurification combined with liquid chromatography high-resolution mass spectrometry

Autor: Michael Groessl, Lia Bally, Jean-Christophe Prost, Gemma Reverter-Branchat, Eric E. Niederkofler, Kwasi Antwi, Christos T. Nakas

Publikováno v: Analytical and Bioanalytical Chemistry
Reverter-Branchat, Gemma; Groessl, Michael; Nakas, Christos T; Prost, Jean-Christophe; Antwi, Kwasi; Niederkofler, Eric E; Bally, Lia (2020). Rapid quantification of insulin degludec by immunopurification combined with liquid chromatography high-resolution mass spectrometry. Analytical and bioanalytical chemistry, 412(30), pp. 8351-8359. Springer 10.1007/s00216-020-02971-4

Insulin degludec is an ultra-long-acting insulin analogue that is increasingly being used in diabetes due to its favourable efficacy and safety profile. Thus, there is an increasing demand for a reliable and specific analytical method to quantify ins

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1817a0169c26b813e75d933f6ac5ce9d
https://pubmed.ncbi.nlm.nih.gov/33006670

Development and validation of a targeted affinity-enrichment and LC-MS/MS proteomics approach for the therapeutic monitoring of adalimumab

Autor: Kiang-Teck J. Yeo, Eric E. Niederkofler, Kwasi Antwi, Eszter Lazar-Molnar, Emily Wysocki, Yifei Yang, Edward Ki Yun Leung

Publikováno v: Clinica chimica acta; international journal of clinical chemistry. 483

Background The anti-tumor necrosis factor alpha (TNFα) therapeutic monoclonal antibodies (mAbs), such as adalimumab, are widely used in the treatment of rheumatoid arthritis, inflammatory bowel diseases, and other auto-immune diseases. The administr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e13acdb5bebba557a5edea9e1f580371
https://pubmed.ncbi.nlm.nih.gov/29752913

Insulin detemir does not cross the human placenta

Autor: Urban A. Kiernan, Gideon Koren, Katarzyna Suffecool, Janelle Foroutan, Priya Bapat, Kwasi Antwi, Barak Rosenn, Eric E. Niederkofler, Amanda Ribar

Publikováno v: Diabetes care. 38(2)

Insulin detemir (IDet) is a relatively new long-acting insulin analog that offers improved absorption pharmacokinetics and slower tissue distribution due to high plasma protein binding (1). As IDet is increasingly used for treatment of insulin-requir

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9203e647553c8edf561d1d74a696fd66
https://pubmed.ncbi.nlm.nih.gov/25614695

Development of Recombinant-Based Mass Spectrometric Immunoassay with Application to Resistin Expression Profiling

Autor: Allan L. Bieber, Kemmons A. Tubbs, Randall W. Nelson, Eric E. Niederkofler, Urban A. Kiernan, Dobrin Nedelkov

Publikováno v: Analytical Chemistry. 78:3271-3276

This report addresses the need for additional assays for human resistin (hRES) by developing a rational progression of the mass spectrometric immunoassay to incorporate recombinant proteins. The recombinant-based hRES mass spectrometric immunoassay (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f683a157f9971d511f47f1c69bf3afa0
https://doi.org/10.1021/ac060013g

High-throughput MS-based protein phenotyping: Application to haptoglobin

Autor: Dobrin Nedelkov, Urban A. Kiernan, Allan L. Bieber, Randall W. Nelson, Eric E. Niederkofler, Kemmons A. Tubbs

Publikováno v: PROTEOMICS. 5:5002-5007

A high-throughput affinity capture and reduction approach was developed for phenotype and post-translational modification analysis of a complexed globular protein, haptoglobin (Hp), directly from human plasma. Hp was selectively retrieved utilizing a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::808ea4e5b7800205176070aed8dfb1d0
https://doi.org/10.1002/pmic.200500176