Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Eric Buel"'
Publikováno v:
ELECTROPHORESIS. 35:3044-3052
This review focuses upon a critical step in forensic biology: detection and quantification of human DNA from biological samples. Determination of the quantity and quality of human DNA extracted from biological evidence is important for several reason
Publikováno v:
Journal of Forensic Sciences. 57:466-471
Knowledge of the degradation state of evidentiary DNA samples would allow selection of the appropriate analysis method (standard short tandem repeats [STRs] vs. mini STRs vs. mtDNA). This article describes the development of a Plexor® technology/rea
Publikováno v:
Journal of Forensic Sciences. 57:478-488
A screening assay has been developed to provide preliminary individualization of crime scene samples thus eliminating expensive, time-consuming short tandem repeat (STR) profiling of nonprobative samples. High resolution melting performed in a real-t
Autor:
Janice A. Nicklas, Eric Buel
Publikováno v:
Journal of Forensic Sciences. 50:1-10
The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more
Publikováno v:
Analytical Chemistry. 68:1941-1947
The observed interlaboratory standard deviation (SD) associated with the restriction fragment length polymorphism (RFLP) measurement of DNA fragment size is a predictable function of the observed mean band size (MBS). For DNA fragments of size 1,000
Autor:
Janice A. Nicklas, Eric Buel
Publikováno v:
Journal of forensic sciences. 53(6)
A method that quickly and inexpensively differentiates crime scene samples from multiple donors would expedite casework analysis by allowing the selection of probative items requiring comprehensive testing. This new method need not be perfectly defin
Publikováno v:
Journal of forensic sciences. 53(4)
Nuclear DNA was extracted from human telogen hairs from 60 individuals. Six to nine hairs from each individual were individually extracted. The amount of DNA recovered from each individual varied greatly, and most samples yielded a quantity of 550 pg
Autor:
Janice A. Nicklas, Eric Buel
Publikováno v:
Journal of forensic sciences. 51(5)
A single duplex assay to determine both the amount of total human DNA and the amount of male DNA in a forensic sample has been developed. This assay is based on TaqMan technology and uses the multicopy Alu sequence to quantitate total human DNA and t
Autor:
Janice A, Nicklas, Eric, Buel
Publikováno v:
Journal of forensic sciences. 50(5)
The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more
Publikováno v:
Electrophoresis. 25(10-11)
DNA typing with short tandem repeat (STR) markers is now widely used for a variety of applications including human identification. Capillary electrophoresis (CE) instruments, such as the ABI Prism 310 and ABI 3100 Genetic Analyzers, are the method of