Výsledky vyhledávání - "Eric B. Kmiec"

Akademický článek

Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas

Autor: Brett M. Sansbury, Amanda M. Hewes, Olivia M. Tharp, Sophia B. Masciarelli, Salma Kaouser, Eric B. Kmiec

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-11 (2022)

Abstract Gene correction is often referred to as the gold standard for precise gene editing and while CRISPR-Cas systems continue to expand the toolbox for clinically relevant genetic repair, mechanistic hurdles still hinder widespread implementation

Externí odkaz: https://doaj.org/article/d54d09c6bc6945aba0290b39b13769d7

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Akademický článek

Genome editing before CRISPR: The gnostic bible

Autor: Eric B. Kmiec

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 31, Iss , Pp 525-526 (2023)

Externí odkaz: https://doaj.org/article/971df95d9b924a93b099bbdaf11f8fe4

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Akademický článek

gRNA Sequence Heterology Tolerance Catalyzed by CRISPR/Cas in an In Vitro Homology-Directed Repair Reaction

Autor: Amanda M. Hewes, Brett M. Sansbury, Shaul Barth, Gabi Tarcic, Eric B. Kmiec

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 20, Iss , Pp 568-579 (2020)

CRISPR and associated Cas nucleases are genetic engineering tools revolutionizing innovative approaches to cancer and inherited diseases. CRISPR-directed gene editing relies heavily on proper DNA sequence alignment between the guide RNA (gRNA)/CRISPR

Externí odkaz: https://doaj.org/article/24aac521f8c14efcafcea27ca77d3465

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Akademický článek

Functional Gene Knockout of NRF2 Increases Chemosensitivity of Human Lung Cancer A549 Cells In Vitro and in a Xenograft Mouse Model

Autor: Pawel Bialk, Yichen Wang, Kelly Banas, Eric B. Kmiec

Publikováno v: Molecular Therapy: Oncolytics, Vol 11, Iss , Pp 75-89 (2018)

Recent studies point to the evolution of drug resistance in lung cancer as being centered, at least in part, on the upregulation of various genes involved in controlling efflux or drug inactivation. Among the most important of these genes is Nuclear

Externí odkaz: https://doaj.org/article/b08f67e4d39b4cea9416503519598f39

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Akademický článek

Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex

Autor: Shirin R. Modarai, Dula Man, Pawel Bialk, Natalia Rivera-Torres, Kevin Bloh, Eric B. Kmiec

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 11, Iss C, Pp 116-129 (2018)

CD34+ cells are prime targets for therapeutic strategies for gene editing, because modified progenitor cells have the capacity to differentiate through an erythropoietic lineage. Although experimental advances have been reported, the associated exper

Externí odkaz: https://doaj.org/article/40039838ea9940a1aab82fc3ebf6d44c

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Akademický článek

CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome

Autor: Kevin M. Bloh, Pawel A. Bialk, Anilkumar Gopalakrishnapillai, E. Anders Kolb, Eric B. Kmiec

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 7, Iss C, Pp 288-298 (2017)

Using a CRISPR/Cas9 system, we have reengineered a translational start site in the GATA1 gene in K562 cells. This mutation accounts largely for the onset of myeloid leukemia in Down syndrome (ML-DS). For this reengineering, we utilized CRISPR/Cas9 to

Externí odkaz: https://doaj.org/article/862e8a7a8bf645a4bd59778c5f829f8a

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Akademický článek

On the Origins of Homology Directed Repair in Mammalian Cells

Autor: Brett M. Sansbury, Eric B. Kmiec

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 7, p 3348 (2021)

Over the course of the last five years, expectations surrounding our capacity to selectively modify the human genome have never been higher. The reduction to practice site-specific nucleases designed to cleave at a unique site within the DNA is now c

Externí odkaz: https://doaj.org/article/832ddb42281a4df285743bb8cfac8698

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Figure S1, Figure S2, Figure S3 from Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells

Autor: Eric B. Kmiec, Byung-Chun Yoo, Pawel Bialk, Natalia Rivera-Torres, Kelly Banas

Figure S1. Fluorescence compensation of mock transfected, GFP- and ATTO 550-fluorescing cells. Figure S2. Representative images used for time course analysis. Figure S3. In vitro cleavage reaction of wildtype and R34G-mutated NRF2 amplicons with vary

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63b3da7d77a5cb7115e48adbf58b0236
https://doi.org/10.1158/1541-7786.22513666.v1

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Deconvolution of Complex DNA Repair (DECODR): Establishing a Novel Deconvolution Algorithm for Comprehensive Analysis of CRISPR-Edited Sanger Sequencing Data

Autor: Kevin Bloh, Zugui Zhang, Eric B. Kmiec, Pawel Bialk, Rohan Kanchana, Kelly Banas, Byung-Chun Yoo

Publikováno v: The CRISPR Journal

During CRISPR-directed gene editing, multiple gene repair mechanisms interact to produce a wide and largely unpredictable variety of sequence changes across an edited population of cells. Shortcomings inherent to previously available proposal-based i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4ec2a9afaf32fee3c39465795c76613
http://europepmc.org/articles/PMC7898406

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