Zobrazeno 1 - 10
of 93
pro vyhledávání: '"Enzyme reconstitution"'
Publikováno v:
ACS Omega
ACS Omega, ACS Publications, 2020, ⟨10.1021/acsomega.9b04034⟩
ACS Omega, Vol 5, Iss 4, Pp 2015-2026 (2020)
ACS Omega, ACS Publications, 2020, ⟨10.1021/acsomega.9b04034⟩
ACS Omega, Vol 5, Iss 4, Pp 2015-2026 (2020)
The ability to switch on the activity of an enzyme through its spontaneous reconstitution has proven to be a valuable tool in fundamental studies of enzyme structure/reactivity relationships or in the design of artificial signal transduction systems
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06651dcd90ca04fef95363822deca315
https://hal.archives-ouvertes.fr/hal-02457126/document
https://hal.archives-ouvertes.fr/hal-02457126/document
Publikováno v:
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Among the currently available virus detection assays, those based on the programmable CRISPR-Cas enzymes have the advantage of rapid reporting and high sensitivity without the requirement of thermocyclers. Type III-A CRISPR-Cas system is a multi-comp
Autor:
Roman Jerala, Tjaša Plaper, Petra Dekleva, Mateja Manček, Fabio Lapenta, Jana Aupič, Mojca Benčina
Publikováno v:
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay. Here, we report the design and versatile applications of orthogon
Autor:
Kole, Ryszard, Altman, Sidney
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 1979 Aug 01. 76(8), 3795-3799.
Externí odkaz:
https://www.jstor.org/stable/69712
Autor:
Strittmatter, Stephen, Neer, Eva J.
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 1980 Nov 01. 77(11), 6344-6348.
Externí odkaz:
https://www.jstor.org/stable/9551
Autor:
Benchao Su, Xing Liu, Zeling Zhang, Qi Chen, Guifang Xie, Yuanyuan Wang, Kunlu Bao, Fujing Mao
Publikováno v:
RSC advances. 10(56)
Ochratoxin A (OTA) is a common cereal mycotoxin that seriously threatens food safety and public health. Herein a horseradish peroxidase-nanobody fusion protein (HRP-Nb) retaining antibody and enzyme activity was obtained after inclusion body denatura
Autor:
Ru Li, Xiaoxia Lv, Minmin Dong, Shuai Li, Shuo Zhang, Yao Jiang, Liyan Zhang, Hua Wang, Lijun Chen
Publikováno v:
Biosensors and Bioelectronics. 87:1036-1043
A facile and efficient enzymatic reconstitution methodology has been proposed for high-catalysis peroxidase mimics by remolding the redox active centers of heme-containing proteins with the in-site biomineralized gold using hemoglobin (Hb) as a model
Autor:
Sathyanarayana N. Gummadi, Vincent Gerard Francis, Abdul M. Mohammed, Gopala Krishna Aradhyam
Publikováno v:
FEBS Journal. 280:2855-2869
Human phospholipid scramblase 1 (hPLSCR1) belongs to the ATP-independent class of phospholipid translocators which possess a single EF-hand-like Ca 2+-binding motif and also a C-terminal helix (CTH). The CTH domain of hPLSCR1 was believed to be a put
Publikováno v:
World Journal of Microbiology and Biotechnology. 28:1185-1191
Pseudomonas aeruginosa is a versatile bacterium that can grow using citronellol or leucine as sole carbon source. For both compounds the degradation pathways converge at the key enzyme 3-methylcrotonyl coenzyme-A carboxylase (MCCase). This enzyme is
Autor:
Akinori Saito, Tsuyoshi Takahashi
Publikováno v:
Chemistry Letters. 43:1357-1359
A new detection method for ligand–protein interactions was constructed using interaction-dependent native chemical ligation and enzyme reconstitution (IDNCL-ER) by employing ribonuclease S. Using a...