Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Enger-Valk BE"'
Autor:
Irma van Die, Simon Barteling, Ingrid van Megen, Hans Bergmans, Betty E. Enger-Valk, Wiel Hoekstra, Frits Mooi, Joost A.W.M. van Oosterhout
Publikováno v:
Moleculargeneral genetics : MGG. 222(2-3)
Hypervariable regions (HRs) of the major subunit of F11 fimbriae were exploited for insertion of foreign epitopes. Two insertion vectors were created that contain a unique cloning site in HR1 or HR4 respectively. Several oligonucleotides, coding for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59648222950293fed5bdc0a5fbe2e0c6
https://pubmed.ncbi.nlm.nih.gov/1703267
https://pubmed.ncbi.nlm.nih.gov/1703267
Publikováno v:
Nucleic Acids Research; 4/15/1981, Vol. 9 Issue 8, p1973-1989, 17p
Autor:
Die, Irma, Oosterhout, Joost, Megen, Ingrid, Bergmans, Hans, Hoekstra, Wiel, Enger-Valk, Betty, Barteling, Simon, Mooi, Frits
Publikováno v:
Molecular & General Genetics MGG; 1990, Vol. 222 Issue 2/3, p297-303, 7p
Autor:
B. E. Enger-Valk, M. P. M. Caspers, G. A. W. De Munk, Jan H. Verheijen, P. H. Pouwels, G. T. G. Chang
Publikováno v:
Biochemistry. 28:7318-7325
Human tissue-type plasminogen activator (t-PA) consists of five domains designated (starting from the N-terminus) finger, growth factor, kringle 1, kringle 2, and protease. The binding of t-PA to lysine-Sepharose and aminohexyl-Sepharose was found to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74f4e7c083351d79890a03051271a226
https://doi.org/10.1021/bi00444a026
https://doi.org/10.1021/bi00444a026
Publikováno v:
Gene. 61:207-215
A series of expression plasmids was constructed to compare the usefulness of various promoters for the synthesis of a given protein in the Saccharomyces cerevisiae. The plasmids pMBL212, -213, -214, -215 and -216 can be used to synthesize the protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2e8189ea76f7c232cee947efe911ad7
https://doi.org/10.1016/0378-1119(87)90115-6
https://doi.org/10.1016/0378-1119(87)90115-6
Publikováno v:
Gene. 9:69-85
In vitro recombination techniques were used to construct a series of new cloning vehicles with genes of the tryptophan (trp) operon of Escherichia coli as selective marker. To construct these plasmids we have made a restriction cleavage map of the tr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29dac4a8b3f92f9bee2ad92c8bb94f42
https://doi.org/10.1016/0378-1119(80)90167-5
https://doi.org/10.1016/0378-1119(80)90167-5
Publikováno v:
Journal of General Virology. 69:1627-1636
A transcription/translation system for generating poliovirus proteins in vitro has been used to assess the proteolytic activity of various polypeptides containing the virus-coded 3C region towards the poliovirus precursor protein P1. Plasmids contain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::273b9b992ba925a7df5ab800f79844b0
https://doi.org/10.1099/0022-1317-69-7-1627
https://doi.org/10.1099/0022-1317-69-7-1627
Autor:
Simon Barteling, Marijke J. Kottenhagen, Ton Blom, Rob H. Meloen, Betty E. Enger-Valk, Peter H. Pouwels, M. P. Broekhuijsen
Publikováno v:
Vaccine. 4:119-124
Part of the genome of foot-and-mouth disease virus (FMDV) type 01,BFS, including the sequence encoding the capsid polypeptide VP1, was cloned in Escherichia coli following a new cloning strategy. The clone containing the VP1 sequence was used for the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ca47598c6c10cec9b17ec414a73875a
https://doi.org/10.1016/0264-410x(86)90050-2
https://doi.org/10.1016/0264-410x(86)90050-2
Autor:
P.H. Pouwels, E A Klasen, J. M. M. Van Rijn, Betty E. Enger-Valk, T Blom, M. P. Broekhuijsen, M J Fasbender
Publikováno v:
Gene. 49:189-197
A series of four expression plasmids coding for fusion proteins containing foot-and-mouth disease virus (FMDV) sequences was constructed. The fusion proteins contain a large part of beta-galactosidase from Escherichia coli preceded (N-terminal) by 1,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80ddaeda1f83cca86ab45230afbc139c
https://doi.org/10.1016/0378-1119(86)90279-9
https://doi.org/10.1016/0378-1119(86)90279-9
Autor:
I Van Megen, Willem Pieter Martin Hoekstra, P Pouwels, H Bergmans, Marca H. M. Wauben, Betty E. Enger-Valk, N Riegman, I van Die
Publikováno v:
Journal of Bacteriology. 170:5870-5876
The influence of genetic manipulation of the structural genes coding for major P-fimbrial subunits on the formation of fimbriae in Escherichia coli was studied. Deletion of two regions that code for hypervariable parts of the P fimbrillin resulted in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::657e8bbaf877cc94622a20194391667c
https://doi.org/10.1128/jb.170.12.5870-5876.1988
https://doi.org/10.1128/jb.170.12.5870-5876.1988