Detection of antigen interactions ex vivo by proximity ligation assay: endogenous dopamine D2-adenosine A2A receptor complexes in the striatum

Autor: Pierre Trifilieff, Marie-Laure Rives, Eneko Urizar, Rebecca A. Piskorowski, Harshad D. Vishwasrao, John Castrillon, Claudia Schmauss, Maria Slättman, Mats Gullberg, Jonathan A. Javitch

Publikováno v: BioTechniques, Vol 51, Iss 2, Pp 111-118 (2011)

The existence of G protein-coupled receptor (GPCR) dimers and/or oligomers has been demonstrated in heterologous systems using a variety of biochemical and biophysical assays. While these interactions are the subject of intense research because of th

Externí odkaz: https://doaj.org/article/ae72400782ba4920bcd825a30c1c6bc0

Increasing dopamine D2 receptor expression in the adult nucleus accumbens enhances motivation

Autor: Eleanor H. Simpson, Holly Moore, Pierre Trifilieff, Peter D. Balsam, Bo Feng, Vanessa Winiger, Kathleen M. Taylor, Ryan D. Ward, Diana Martinez, Jonathan A. Javitch, Eneko Urizar

Publikováno v: Molecular Psychiatry
Molecular Psychiatry, 2013, 18 (9), pp.1025—1033. ⟨10.1038/mp.2013.57⟩

A decrease in dopamine D2 receptor (D2R) binding in the striatum is one of the most common findings in disorders that involve a dysregulation of motivation, including obesity, addiction, and attention deficit hyperactivity disorder. Since disruption

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5da50e826fee4637f9ab65cb85559466
https://doi.org/10.1038/mp.2013.57

G Protein-coupled Receptor Kinase-2 Constitutively Regulates D2 Dopamine Receptor Expression and Signaling Independently of Receptor Phosphorylation

Autor: David R. Sibley, Yoon Namkung, Eneko Urizar, Concetta Dipace, Jonathan A. Javitch

Publikováno v: Journal of Biological Chemistry. 284:34103-34115

We investigated the regulatory effects of GRK2 on D(2) dopamine receptor signaling and found that this kinase inhibits both receptor expression and functional signaling in a phosphorylation-independent manner, apparently through different mechanisms.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5db36536336be0bc67aa52e5af521ba5
https://doi.org/10.1074/jbc.m109.055707

Evidence against dopamine D1/D2 receptor heteromers

Autor: Roger J. Colbran, Devon L. Graham, Kai C. Sonntag, Hideaki Yano, Harshad D. Vishwasrao, Pierre Trifilieff, Jozsef Meszaros, Christoph Kellendonk, David R. Sibley, Eneko Urizar, Dominik Biezonski, Jonathan A. Javitch, Gregg D. Stanwood, Aliya L. Frederick

Publikováno v: Molecular Psychiatry
Molecular Psychiatry, Nature Publishing Group, 2015, 20 (11), pp.1373-1385. ⟨10.1038/mp.2014.166⟩
Molecular psychiatry

International audience; Hetero-oligomers of G-protein-coupled receptors have become the subject of intense investigation, because their purported potential to manifest signaling and pharmacological properties that differ from the component receptors

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94302edeeeed42d80e2f446f4179e6f0
https://hal.inrae.fr/hal-02636137

Glycoprotein hormone receptors: link between receptor homodimerization and negative cooperativity

Autor: Marco Bonomi, Tiffany Loy, Michel Bouvier, Sabine Costagliola, Gilbert Vassart, Guillaume Smits, Stéphane Swillens, Eneko Urizar, Lucia Montanelli, Céline Galés

Publikováno v: The EMBO Journal. 24:1954-1964

The monomeric model of rhodopsin-like G protein-coupled receptors (GPCRs) has progressively yielded the floor to the concept of GPCRs being oligo(di)mers, but the functional correlates of dimerization remain unclear. In this report, dimers of glycopr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddef317c06dc28c069b3454e78a5bdd9
https://doi.org/10.1038/sj.emboj.7600686