Zobrazeno 1 - 10
of 431
pro vyhledávání: '"Endogenous control"'
Autor:
Fabiana Sassù, Barbora Vomáčková Kykalová, Cecilia Stahl Vieira, Petr Volf, Erich Loza Telleria
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-15 (2024)
Abstract We investigated gene expression patterns in Lutzomyia and Phlebotomus sand fly vectors of leishmaniases. Using quantitative PCR, we assessed the expression stability of potential endogenous control genes commonly used in dipterans. We analyz
Externí odkaz:
https://doaj.org/article/ea059f6ec5f54def9a7b405c2e7c1eb1
Autor:
Miranda Burdiel, Julia Jiménez, Carlos Rodríguez-Antolín, Álvaro García-Guede, Olga Pernía, Ana Sastre-Perona, Rocío Rosas-Alonso, Julián Colmenarejo, Carmen Rodríguez-Jiménez, María Dolores Diestro, Virginia Martínez-Marín, Oliver Higueras, Patricia Cruz, Itsaso Losantos-García, Héctor Peinado, Olga Vera, Javier de Castro, Inmaculada Ibáñez de Cáceres
Publikováno v:
Biomarker Research, Vol 11, Iss 1, Pp 1-5 (2023)
Abstract Small extracellular vesicles (sEVs) in the blood of cancer patients contain higher amounts of tumor markers than those identified as free-circulating. miRNAs have significant biomedical relevance due to their high stability and feasible dete
Externí odkaz:
https://doaj.org/article/fb3fd8538ff04f0a980527d02e7153c5
Publikováno v:
HortScience, Vol 58, Iss 8, Pp 819-824 (2023)
The pathogen Xylella fastidiosa is a xylem-restricted, gram-negative bacterium that is known to cause diseases of many cultivated plant species. Recent outbreaks of X. fastidiosa diseases in Europe have brought attention to the impact of this pathoge
Externí odkaz:
https://doaj.org/article/c7356f1d0da24d608e9cca72e62366a8
Akademický článek
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Autor:
T. Stverakova, I. Baranova, P. Mikyskova, B. Gajdosova, H. Vosmikova, J. Laco, V. Palicka, H. Parova
Publikováno v:
Frontiers in Oncology, Vol 13 (2023)
IntroductionCervical cancer causes approximately 350,000 deaths each year. The availability of sensitive and specific diagnostic tests to detect cervical cancer in its early stages is essential to improve survival rates.MethodsIn this study, we compa
Externí odkaz:
https://doaj.org/article/9b3b6d28660a43eca10b0430f85bf3e6
Publikováno v:
Heliyon, Vol 9, Iss 4, Pp e14515- (2023)
Circulating miRNA expression is most commonly measured by qRT-PCR, however, the lack of a suitable endogenous control hinders people from evaluating the accurate changes in miRNA expression levels and developing the non-invasive biomarkers. In this s
Externí odkaz:
https://doaj.org/article/fb90e4d6688341c5abf7f2ec223dd8bc
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
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Autor:
Daina Pamedytyte, Enrika Leipute, Birute Zilaitiene, Valdas Sarauskas, Dalia Dauksiene, Albertas Dauksa, Aurelija Zvirbliene
Publikováno v:
Molecular Medicine, Vol 26, Iss 1, Pp 1-7 (2020)
Abstract Background The most popular miRNA quantitation technique is RQ-PCR with relative gene expression method that requires an endogenous control (EC) gene for data normalization. However, there are insufficient data and selection criteria on the
Externí odkaz:
https://doaj.org/article/c345895547064aeaab61379c857a959e
Autor:
Cecilia Mittelberger, Lisa Obkircher, Vicky Oberkofler, Alan Ianeselli, Christine Kerschbamer, Andreas Gallmetzer, Yazmid Reyes-Dominguez, Thomas Letschka, Katrin Janik
Publikováno v:
Plant Methods, Vol 16, Iss 1, Pp 1-14 (2020)
Abstract Background Phytoplasma are obligate intracellular plant-pathogenic bacteria that infect a broad range of plant species and are transmitted by different insect species. Quantitative real-time PCR (qPCR) is one of the most commonly used techni
Externí odkaz:
https://doaj.org/article/67201376d8ea4d58830b47781e487ce4
Publikováno v:
Frontiers in Genetics, Vol 13 (2022)
Introduction: MicroRNAs are small noncoding RNAs with potential regulatory roles in hypertension and drug response. The presence of many of these RNAs in biofluids has spurred investigation into their role as possible biomarkers for use in precision
Externí odkaz:
https://doaj.org/article/b7de66e7f302423db23199d597570827