Zobrazeno 1 - 10
of 171
pro vyhledávání: '"Emmanuelle Charpentier"'
Autor:
Nina Lautenschläger, Katja Schmidt, Carolin Schiffer, Thomas F. Wulff, Karin Hahnke, Knut Finstermeier, Moïse Mansour, Alexander K. W. Elsholz, Emmanuelle Charpentier
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen Streptococ
Externí odkaz:
https://doaj.org/article/ab38edfc233547cf9549c6c011dab4ff
Autor:
Vanessa Muñoz-Gutierrez, Fabián A. Cornejo, Katja Schmidt, Christian K. Frese, Manuel Halte, Marc Erhardt, Alexander K. W. Elsholz, Kürşad Turgay, Emmanuelle Charpentier
Publikováno v:
mSystems, Vol 9, Iss 4 (2024)
ABSTRACTInitiation of bacterial DNA replication takes place at the origin of replication (oriC), a region characterized by the presence of multiple DnaA boxes that serve as the binding sites for the master initiator protein DnaA. This process is tigh
Externí odkaz:
https://doaj.org/article/c289fa04900e4f13b86da90c843c68c5
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-13 (2023)
Abstract The Gram-negative bacteria Salmonella enterica and Escherichia coli are important model organisms, powerful prokaryotic expression platforms for biotechnological applications, and pathogenic strains constitute major public health threats. To
Externí odkaz:
https://doaj.org/article/b60852bea5854f32a7e705e857316662
Autor:
Svenja Hüsing, Manuel Halte, Ulf van Look, Alina Guse, Eric J. C. Gálvez, Emmanuelle Charpentier, David F. Blair, Marc Erhardt, Thibaud T. Renault
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Type-III secretion systems (T3SSs) are capable of translocating proteins with high speed while maintaining the membrane barrier for small molecules. Here, a structure-function analysis of the T3SS pore complex elucidates the precise mechanisms enabli
Externí odkaz:
https://doaj.org/article/b4b81677e4d248c0b18979bbe7b55964
Autor:
Laura Broglia, Anne-Laure Lécrivain, Thibaud T. Renault, Karin Hahnke, Rina Ahmed-Begrich, Anaïs Le Rhun, Emmanuelle Charpentier
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Bacterial RNA degradation is typically initiated by endoribonucleases and followed by exoribonucleases. Here the authors report the targetome of endoRNase Y in Streptococcus pyogenes, revealing the interplay between RNase Y and 3′-to-5′ exoribonu
Externí odkaz:
https://doaj.org/article/91cd2b74eef244269a407fef7ba055c4
Autor:
Marlène S. Birk, Rina Ahmed-Begrich, Stefan Tran, Alexander K. W. Elsholz, Christian K. Frese, Emmanuelle Charpentier
Publikováno v:
mSystems, Vol 6, Iss 4 (2021)
ABSTRACT The cellular proteome comprises all proteins expressed at a given time and defines an organism’s phenotype under specific growth conditions. The proteome is shaped and remodeled by both protein synthesis and protein degradation. Here, we d
Externí odkaz:
https://doaj.org/article/cb9a97c6f011478a9ae1eebe9aa2e346
Autor:
Emmanuelle Charpentier
Publikováno v:
Molecular Frontiers Journal, Vol 1, Iss 2, Pp 99-107 (2017)
The RNA-programmable CRISPR-Cas9 technology allows precise and efficient engineering or correction of mutations, modulation of gene expression and marking of DNA in a wide variety of cell types and organisms in the three domains of life. Because of i
Externí odkaz:
https://doaj.org/article/ec9fad94453c466d8b1b87c063b19c1c
Autor:
Dior Beerens, Sandra Franch-Arroyo, Timothy J. Sullivan, Christian Goosmann, Volker Brinkmann, Emmanuelle Charpentier
Publikováno v:
Viruses, Vol 13, Iss 4, p 612 (2021)
Bacteriophages exert strong evolutionary pressure on their microbial hosts. In their lytic lifecycle, complete bacterial subpopulations are utilized as hosts for bacteriophage replication. However, during their lysogenic lifecycle, bacteriophages can
Externí odkaz:
https://doaj.org/article/3a262b2a412141c6868c3dd4694ce4d2
Autor:
Florian D Fabiani, Thibaud T Renault, Britta Peters, Tobias Dietsche, Eric J C Gálvez, Alina Guse, Karen Freier, Emmanuelle Charpentier, Till Strowig, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner, Michael Hensel, Marc Erhardt
Publikováno v:
PLoS Biology, Vol 15, Iss 8, p e2002267 (2017)
Many bacteria move using a complex, self-assembling nanomachine, the bacterial flagellum. Biosynthesis of the flagellum depends on a flagellar-specific type III secretion system (T3SS), a protein export machine homologous to the export machinery of t
Externí odkaz:
https://doaj.org/article/971ccf46c2bb48bc9748a5e4db21f942
Publikováno v:
Frontiers in Molecular Biosciences, Vol 4 (2017)
Here, we review the diverse roles and functions of AAA+ protease complexes in protein homeostasis, control of stress response and cellular development pathways by regulatory and general proteolysis in the Gram-positive model organism Bacillus subtili
Externí odkaz:
https://doaj.org/article/c173756ef83446b08a05439882d89953