Interaction of Human β-Defensin 2 (HBD2) with Glycosaminoglycans

Autor: Dušan Uhrín, Dominic J. Campopiano, Emily S. Seo, Perdita E. Barran, Bärbel S. Blaum, Thomas Vargues, Malcolm Lyon, Martin De Cecco, Jon A. Deakin

Publikováno v: Seo, E S, Blaum, B S, Vargues, T, De Cecco, M, Deakin, J A, Lyon, M, Barran, P E, Campopiano, D J & Uhrín, D 2010, ' Interaction of Human beta-Defensin 2 (HBD2) with Glycosaminoglycans ', Biochemistry, vol. 49, no. 49, pp. 10486–10495 . https://doi.org/10.1021/bi1011749

Human β-defensin 2 (HBD2) is a member of the defensin family of antimicrobial peptides that plays important roles in the innate and adaptive immune system of both vertebrates and invertebrates. In addition to their direct bactericidal action, defens

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e9bdc37c9a2bd4ffcd252afd57dfd82
https://doi.org/10.1021/bi1011749

Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli

Autor: Thomas Vargues, David Clarke, Fiona Kilanowski, John R. W. Govan, Uday Pathania, C. Logan Mackay, Emily S. Seo, Julien Bennani, Julia R. Dorin, Gareth J. Morrison, Helen L. Fielder, Dušan Uhrín, Dominic J. Campopiano

Publikováno v: Protein & Peptide Letters. 16:668-676

Human β-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2e7b3620def1cd0b5941671a87b9bfd2
https://doi.org/10.2174/092986609788490122

Optimal Attachment Position and Linker Length Promote Native-like Character of Cavitand-Based Template-Assembled Synthetic Proteins (TASPs)

Autor: Suzana K. Straus, Walter R. P. Scott, John C. Sherman, Emily S. Seo

Publikováno v: Chemistry - A European Journal. 13:3596-3605

We have designed, synthesised and characterised a series of template-assembled de novo four-helix bundles, each differing in the linker length between the template and the peptides. The helix is based on an earlier peptide sequence: EELLKKLEELLKKLG (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b99b0488c335c360a0ecdcce55d152ef
https://doi.org/10.1002/chem.200601784

Peptide Fragments of a β-Defensin Derivative with Potent Bactericidal Activity ▿

Autor: Julia R. Dorin, Dušan Uhrín, Dominic J. Campopiano, Jason M. D. Kalapothakis, Martin De Cecco, Emily S. Seo, John R. W. Govan, Fiona Kilanowski, Natalie L. Reynolds, Chloe Stanton, Perdita E. Barran, Derek Macmillan, K. J. Taylor

Publikováno v: Reynolds, N L, De Cecco, M, Taylor, K, Stanton, C, Kilanowski, F, Kalapothakis, J, Seo, E, Uhrin, D, Campopiano, D, Govan, J, Macmillan, D, Barran, P & Dorin, J R 2010, ' Peptide Fragments of a β-Defensin Derivative with Potent Bactericidal Activity ', Antimicrobial Agents and Chemotherapy, vol. 54, no. 5, pp. 1922-1929 . https://doi.org/10.1128/AAC.01568-09

β-Defensins are known to be both antimicrobial and able to chemoattract various immune cells. Although the sequences of paralogous genes are not highly conserved, the core defensin structure is retained. Defb14-1C V has bactericidal activity similar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::207f6c8e55806a61e98f4b586696a90c
https://europepmc.org/articles/PMC2863616/

Preparation of isotopically labelled recombinant beta-defensin for NMR studies

Autor: David Clarke, Thomas Vargues, Dušan Uhrín, Dominic J. Campopiano, Emily S. Seo

Publikováno v: Seo, E S, Vargues, T, Clarke, D J, Uhrin, D & Campopiano, D J 2009, ' Preparation of isotopically labelled recombinant β-defensin for NMR studies ', Protein Expression and Purification, vol. 65, no. 2, pp. 179-184 . https://doi.org/10.1016/j.pep.2008.11.007

beta-Defensins are a family of cationic peptides that contain six invariant cysteine residues that form characteristic disulfide bonds between Cys1-Cys5, Cys2-Cys4 and Cys3-Cys6. They have been shown to act as potent antimicrobial agents and chemokin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4d85b02706e311be06f0d849a524202
https://pubmed.ncbi.nlm.nih.gov/19063971