Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Emily K Schutsky"'
Publikováno v:
ACS Chem Biol
Human APOBEC3A (A3A) is a nucleic acid-modifying enzyme that belongs to the cytidine deaminase family. Canonically, A3A catalyzes the deamination of cytosine into uracil in single-stranded DNA, an activity that makes A3A both a critical antiviral def
Autor:
Emily B. Fabyanic, Rahul M. Kohli, Monica Yun Liu, Young Hwang, Hao Wu, Christopher S. Nabel, Jamie E. DeNizio, Emily K Schutsky, Frederic D. Bushman, Peng Hu
Publikováno v:
Nature biotechnology
Here we present APOBEC-coupled epigenetic sequencing (ACE-seq), a bisulfite-free method for localizing 5-hydroxymethylcytosine (5hmC) at single-base resolution with low DNA input. The method builds on the observation that AID/APOBEC family DNA deamin
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2198
Here, we provide a detailed protocol for our previously published technique, APOBEC-Coupled Epigenetic Sequencing (ACE-Seq), which localizes 5-hydroxymethylcytosine at single nucleotide resolution using nanogram quantities of input genomic DNA. In ad
Publikováno v:
Methods in Molecular Biology ISBN: 9781071608753
Here, we provide a detailed protocol for our previously published technique, APOBEC-Coupled Epigenetic Sequencing (ACE-Seq), which localizes 5-hydroxymethylcytosine at single nucleotide resolution using nanogram quantities of input genomic DNA. In ad
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3fd8ce86b94ba2b5566760eb1abc9fa6
https://doi.org/10.1007/978-1-0716-0876-0_27
https://doi.org/10.1007/978-1-0716-0876-0_27
Publikováno v:
Current Opinion in Chemical Biology. 45:10-17
The introduction of site-specific DNA modifications to the genome or epigenome presents great opportunities for manipulating biological systems. Such changes are now possible through the combination of DNA-modifying enzymes with targeting modules, in
Publikováno v:
Nucleic Acids Research
AID/APOBEC family enzymes are best known for deaminating cytosine bases to uracil in single-stranded DNA, with characteristic sequence preferences that can produce mutational signatures in targets such as retroviral and cancer cell genomes. These dea
Publikováno v:
Nature structuralmolecular biology. 24(2)
Targeted deamination of cytosine bases in DNA by AID/APOBEC-family enzymes is critical for proper immune function, but it also poses risks to genomic integrity. New structures reported by Harris, Aihara and colleagues offer the first glimpses into th
Publikováno v:
Journal of Molecular Biology. 423:736-751
Lysosomal enzymes catalyze the breakdown of macromolecules in the cell. In humans, loss of activity of a lysosomal enzyme leads to an inherited metabolic defect known as a lysosomal storage disorder. The human lysosomal enzyme galactosamine-6-sulfata
Chemical modifications to genomic DNA can expand and shape its coding potential. Cytosine methylation in particular has well-established roles in regulating gene expression and defining cellular identity. The discovery of TET family enzymes opened a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53d636381c9ee0dae370441c4c158cbf
https://europepmc.org/articles/PMC5018417/
https://europepmc.org/articles/PMC5018417/
Publikováno v:
Cell Cycle. 13:171-172
Purposeful genomic mutation mediates critical aspects of both adaptive and innate immunity. A physiological role for purposeful mutation is best illustrated by the AID/APOBEC family of enzymes, which can deaminate cytosine to introduce rogue uracil b