Systematic dissection and trajectory-scanning mutagenesis of the molecular interface that ensures specificity of two-component signaling pathways.

Autor: Emily J Capra, Barrett S Perchuk, Emma A Lubin, Orr Ashenberg, Jeffrey M Skerker, Michael T Laub

Publikováno v: PLoS Genetics, Vol 6, Iss 11, p e1001220 (2010)

Two-component signal transduction systems enable bacteria to sense and respond to a wide range of environmental stimuli. Sensor histidine kinases transmit signals to their cognate response regulators via phosphorylation. The faithful transmission of

Externí odkaz: https://doaj.org/article/8a527c48032a4a7a98431136d353efc7

Spatial tethering of kinases to their substrates relaxes evolutionary constraints on specificity

Autor: Michael T. Laub, Hana R. Snow, Jeffrey M. Skerker, Charlotte A. Seid, Barrett S. Perchuk, Emily J. Capra, Orr Ashenberg

Publikováno v: Molecular Microbiology. 86:1393-1403

Summary Signal transduction proteins are often multi-domain proteins that arose through the fusion of previously independent proteins. How such a change in the spatial arrangement of proteins impacts their evolution and the selective pressures acting

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::34766e59c91b8c6dff712848e4331698
https://doi.org/10.1111/mmi.12064

A new system for comparative functional genomics of Saccharomyces yeasts

Autor: Sarah J. Pfau, Peter A. Combs, Carmen Young, J. Scott Breunig, Yufei Liu, Sandhya Sinha, Maitreya J. Dunham, Rosa M. Mendoza, Max V. Staller, Aaron Hoisos, Richa Gawande, Konrad J. Karczewski, Julia Liu, Jie Yuan, Erin C. Bush, Namita Bisaria, Christian Millian, Hugo Arellano, Daniel Gadala-Maria, Samuel Grossberg, Evgeni M. Frenkel, William Rounds, Christopher A. Crutchfield, Alicia P. Hayes, David S. Glass, Walter Chen, Joy Agee, Chris DeSevo, Matthew S. Rich, Jenny Hsi, Philip Stern, Trevor Martin, Sanford J. Silverman, Sandeep Raj, Mina L Kojima, Christopher D. Doucette, Matthew Salesi, Sachi Inukai, Samuel S. Freeman, Olga G. Troyanskaya, Anita Gupta, David Botstein, Juan R. Alvarez-Dominguez, Xiaobo Ke, Cameron Myhrvold, Peter Fellowes, Manuel Llinás, Rajani Sharma, John J. Clore, Maxwell Weidmann, Amy A. Caudy, Coleen T. Murphy, Patrick H. Bradley, Christina Hansen, Yue Jia, David A. Cappel, Joseph Rokicki, Olukunle O. Demuren, Samuel Leachman, Daniel R. Barrett, Hanlin Tang, Anna Liebowitz, Yuanfang Guan, Cynthia Bauerle, Jose Mena, Michael Salazar, Cara Singer, Sharon Weeks, Ashley R. Wolf, Megan N. McClean, Emily J. Capra, Latanya Hammonds-Odie, Daniel S. Lieber, Yu-Han Huang Hsu

Publikováno v: Genetics

Whole-genome sequencing, particularly in fungi, has progressed at a tremendous rate. More difficult, however, is experimental testing of the inferences about gene function that can be drawn from comparative sequence analysis alone. We present a genom

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43a8e75cea2960982b0bbf0c60bfe6de
https://pubmed.ncbi.nlm.nih.gov/23852385

Adaptive mutations that prevent cross-talk enable the expansion of paralogous signaling protein families

Autor: Barrett S. Perchuk, Michael T. Laub, Jeffrey M. Skerker, Emily J. Capra

SUMMARY Orthologous proteins often harbor numerous substitutions, but whether these differences result from neutral or adaptive processes is usually unclear. To tackle this challenge, we examined the divergent evolution of a model bacterial signaling

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22bfbc5300b9e7add639131bc7af539c
https://europepmc.org/articles/PMC3415470/

Comparative developmental expression profiling of two C. elegans isolates

Autor: Sonja M. Skrovanek, Emily J. Capra, Leonid Kruglyak

Publikováno v: PloS one, vol 3, iss 12
PLoS ONE
PLoS ONE, Vol 3, Iss 12, p e4055 (2008)

Gene expression is known to change during development and to vary among genetically diverse strains. Previous studies of temporal patterns of gene expression during C. elegans development were incomplete, and little is known about how these patterns

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59758e960b7f976e4bd03c1c72d17d34
https://escholarship.org/uc/item/7hn0192g