Design, Expression, Purification, and Characterization of a YFP-Tagged 2019-nCoV Spike Receptor-Binding Domain Construct

Autor: Tobias Bierig, Gabriella Collu, Alain Blanc, Emiliya Poghosyan, Roger M. Benoit

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)

2019-nCoV is the causative agent of the serious, still ongoing, worldwide coronavirus disease (COVID-19) pandemic. High quality recombinant virus proteins are required for research related to the development of vaccines and improved assays, and to th

Externí odkaz: https://doaj.org/article/f0daf131130c4df3af55b33ab0a1cd72

Cryo-EM structure of a single-chain β1-adrenoceptor – AmpC β-lactamase fusion protein

Autor: Roger Benoit, Tobias Bierig, Spencer Bliven, Aleix Lafita, Emiliya Poghosyan, Gabriella Collu, Inayathulla Mohammed

The insertion of fusion proteins has enabled the crystallization of a wide range of G-protein-coupled receptors (GPCRs). Here, we explored the possibility of using a larger fusion protein, inserted into the third intracellular loop (ICL3) of β1-adre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c4c8f7ac2a3bc0a4c41c1c8038adbef0
https://doi.org/10.1101/2021.09.25.461805

Correlation between Oxygen Vacancies and Oxygen Evolution Reaction Activity for a Model Electrode: PrBaCo2O5+δ

Autor: Elena Marelli, Emiliya Poghosyan, Denis Sheptyakov, Elisabeth Müller, Marisa Medarde, Emiliana Fabbri, Jaume Gazquez, Dino Aegerter, Cinthia Piamonteze, Thomas J. Schmidt, Ekaterina Pomjakushina, Dariusz Jakub Gawryluk

Publikováno v: Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Digital.CSIC. Repositorio Institucional del CSIC
instname

The role of the perovskite lattice oxygen in the oxygen evolution reaction (OER) is systematically studied in the PrBaCo2O5+δ family. The reduced number of physical/chemical variables combined with in-depth characterizations such as neutron dif-frac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3ce0d7f236226bc05e23be978e57f4b
http://hdl.handle.net/10261/244735

Chimeric single α-helical domains as rigid fusion protein connections for protein nanotechnology and structural biology

Autor: Vincent Olieric, Anna-Sophia Krebs, Gabriella Collu, Sylvain Engilberge, Tobias Bierig, Emiliya Poghosyan, Niveditha Varma, Xavier Deupi, Roger Benoit, Ramon Guixà-González

SummaryChimeric fusion proteins are essential tools for protein nanotechnology. Non-optimized protein-protein connections are usually flexible, which makes them unsuitable as structural building blocks. Here we show that the ER/K motif, a single α-h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::30349293abed4c7e9aa3c59eabf62ab7
https://doi.org/10.1101/2020.09.29.318410

The structure and symmetry of radial spoke protein complex in Chlamydomonas flagella

Autor: Alexander Leitner, Ruedi Aebersold, Takashi Ishikawa, Ioan Iacovache, Benoît Zuber, Emiliya Poghosyan, Pinfen Yang, Dennis R. Diener, Lenka Faltova

Publikováno v: Journal of Cell Science.

The radial spoke is a key element in a transducer apparatus controlling the motility of eukaryotic cilia. The transduction biomechanics is a long-standing question in cilia biology. The radial spoke has three regions – a spoke head, a bifurcated ne

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8a24ac6cd8a84ea166cee9385686b9fa
https://doi.org/10.1242/jcs.245233