The TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis

Autor: Hélène Scheer, Caroline de Almeida, Emilie Ferrier, Quentin Simonnot, Laure Poirier, David Pflieger, François M. Sement, Sandrine Koechler, Christina Piermaria, Paweł Krawczyk, Seweryn Mroczek, Johana Chicher, Lauriane Kuhn, Andrzej Dziembowski, Philippe Hammann, Hélène Zuber, Dominique Gagliardi

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)

TUTase mediated uridylation of mRNA promotes degradation. Here, Scheer, de Almeida et al. show that Arabidopsis TUTase URT1 interacts directly with the translation inhibitor and decay factor DECAPPING5 and suppresses siRNA biogenesis by preventing ac

Externí odkaz: https://doaj.org/article/d26320e63277491b9c09179e1c48bfd5

The TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis

Autor: Emilie Ferrier, Philippe Hammann, Christina Piermaria, Pawel S. Krawczyk, Laure Poirier, Dominique Gagliardi, Hélène Zuber, Quentin Simonnot, François M. Sement, Johana Chicher, Caroline de Almeida, Andrzej Dziembowski, Seweryn Mroczek, Sandrine Koechler, Hélène Scheer, David Pflieger, Lauriane Kuhn

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-021-21382-2⟩

Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular netw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3c14c1dbe381aa1986d6ed5ccfc0da5
https://doi.org/10.1038/s41467-021-21382-2

Uridylation prevents 3' trimming of oligoadenylated mRNAs

Autor: Heike Lange, Rémy Merret, Cécile Bousquet-Antonelli, Malek Alioua, Dominique Gagliardi, Hélène Zuber, François M. Sement, Jean-Marc Deragon, Emilie Ferrier

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2013, 41 (14), pp.7115-7127
HAL
Nucleic Acids Research, Oxford University Press, 2013, 41 (14), pp.7115-7127. ⟨10.1093/nar/gkt465⟩

Degradation of mRNAs is usually initiated by deadenylation, the shortening of long poly(A) tails to oligo(A) tails of 12-15 As. Deadenylation leads to decapping and to subsequent 5' to 3' degradation by XRN proteins, or alternatively 3' to 5' degrada

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3d69198610e1cd3e4bf1f60991e7746
https://hal.archives-ouvertes.fr/hal-00862569