Purification of human butyrylcholinesterase from frozen Cohn fraction IV-4 by ion exchange and Hupresin affinity chromatography.

Autor: Lawrence M Schopfer, Oksana Lockridge, Emilie David, Steven H Hinrichs

Publikováno v: PLoS ONE, Vol 14, Iss 1, p e0209795 (2019)

Human butyrylcholinesterase (HuBChE) is being developed as a therapeutic for protection from the toxicity of nerve agents. An enriched source of HuBChE is Cohn fraction IV-4 from pooled human plasma. For the past 40 years, purification of HuBChE has

Externí odkaz: https://doaj.org/article/affc078c1bbb4040bab862cd9ed54a21

Organocatalyzed Sulfa-Michael Addition of Thiophenols on Trisubstituted α-Fluoroacrylates, a Straightforward Access to Chiral Fluorinated Compounds

Autor: Samuel Couve-Bonnaire, Philippe Jubault, Tatiana Besset, Xin Huang, Emilie David

Publikováno v: Journal of Organic Chemistry
Journal of Organic Chemistry, American Chemical Society, 2020, 85 (21), pp.14055-14067. ⟨10.1021/acs.joc.0c02081⟩

International audience; In this manuscript, a simple and efficient sulfa-Michael addition reaction of aryl thiols to trisubstituted α-fluoro-α,β-unsaturated esters both in racemic and, for the first time, in enantioselective version is reported. T

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28e78eb796d74c0853e1c61279e93c0b
https://hal.archives-ouvertes.fr/hal-03004699/document

Comparison of Hupresin® to Procainamide-Sepharose for Purification of Butyrylcholinesterase and Acetylcholinesterase

Autor: Emilie David, Florian Nachon, Oksana Lockridge, Xavier Brazzolotto, Lawrence M. Schopfer, Patrick Masson

Affinity chromatography on procainamide-Sepharose has been an important step in the purification of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) since its introduction in 1978. The procainamide affinity gel has limitations. In the pre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::60512228c0f94e7ec3f2bf9810f93163
https://doi.org/10.20944/preprints201806.0443.v1

Ethyl dibromofluoroacetate: a versatile reagent for the synthesis of fluorinated molecules

Autor: Xavier Pannecoucke, Philippe Jubault, Emilie David, Samuel Couve-Bonnaire

Publikováno v: Tetrahedron
Tetrahedron, Elsevier, 2013, 69 (52), pp.11039-11055. ⟨10.1016/j.tet.2013.10.048⟩

International audience

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b205182c22cb019fb4b5ec5509b0f7f5
http://europepmc.org/articles/PMC7126840

Copper catalyzed 1,3-dipolar cycloaddition reaction of azides with N-(2-trifluoroacetylaryl)propargylamines

Autor: Marc Lemaire, Emilie David, Guillaume Pilet, Maurice Médebielle, Etsuji Okada, Sandrine Lassara, Jerome Molette, Jean-Florent Lamarque, Dai Shibata, Christophe Lamarque, Stéphane Pellet-Rostaing

Publikováno v: Journal of Fluorine Chemistry. 129:788-798

The synthesis of N -(2-trifluoroacetylaryl)propargylamines 10 – 14 and 17 is presented. The copper(I) catalyzed cycloaddition reaction of these propargylamines (dipolarophiles) with a series of azides (1,3-dipoles) 18 – 20 , 21 and 24 was found t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::59f53bfac39b65824eb6a41153c3d613
https://doi.org/10.1016/j.jfluchem.2008.05.015