Deagglomeration of DNA nanomedicine carriers using controlled ultrasonication

Autor: Beth A. Hinchliffe, Piers Turner, David J. H. Cant, Emiliana De Santis, Purnank Aggarwal, Rob Harris, David Templeton, Alex G. Shard, Mark Hodnett, Caterina Minelli

Publikováno v: Ultrasonics Sonochemistry, Vol 89, Iss , Pp 106141- (2022)

Control over the agglomeration state of manufactured particle systems for drug and oligonucleotide intracellular delivery is paramount to ensure reproducible and scalable therapeutic efficacy. Ultrasonication is a well-established mechanism for the d

Externí odkaz: https://doaj.org/article/feb3dcf8a7e94f63b09eea42f743ec28

Imaging and 3D Reconstruction of De Novo Peptide Capsids

Autor: Emiliana, De Santis, Maxim G, Ryadnov

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2208

Nanoscale systems encapsulating biomacromolecules hold promise for cell and gene therapies. Common issues hampering progress include polydispersity, heterogeneity in size and shape, agglomeration, and poor stability. Much attention is given to the se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fdce479b77e708c2ac13ab3c2cfcd28e
https://pubmed.ncbi.nlm.nih.gov/32856261

Engineering Chirally Blind Protein Pseudocapsids into Antibacterial Persisters

Autor: Maxim G. Ryadnov, Smita B. Gunnoo, Franca Fraternali, Xiaoliang Ba, Mark A. Holmes, Emiliana De Santis, Helen Lewis, Ibolya E. Kepiro, Michael Shaw, Katharine Hammond, Urszula Łapińska, Stefano Pagliara, Irene Marzuoli, Bart W. Hoogenboom, Christian D. Lorenz

Publikováno v: Kepiro, I E, Marzuoli, I, Hammond, K, Ba, X, Lewis, H, Shaw, M, Gunnoo, S B, De Santis, E, Łapińska, U, Pagliara, S, Holmes, M A, Lorenz, C D, Hoogenboom, B W, Fraternali, F & Ryadnov, M G 2020, ' Engineering Chirally Blind Protein Pseudocapsids into Antibacterial Persisters ', ACS Nano, vol. 14, no. 2, pp. 1609-1622 . https://doi.org/10.1021/acsnano.9b06814

Antimicrobial resistance stimulates the search for antimicrobial forms that may be less subject to acquired resistance. Here we report a conceptual design of protein pseudocapsids exhibiting a broad spectrum of antimicrobial activities. Unlike conven

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcd239e06e6c0b17813655bfee76ba2c
https://www.repository.cam.ac.uk/handle/1810/301228

Imaging Protein Fibers at the Nanoscale and In Situ

Autor: Angelo, Bella, Michael, Shaw, Emiliana, De Santis, Maxim G, Ryadnov

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1777

Protein self-assembly offers a rich repertoire of tools and technologies. However, despite significant progress in this area, a deterministic measure of the phenomenon, which might lead to predictable relationships between protein components, assembl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7e9e7bfe0a1fcc7ab8c7f5320112cf6c
https://pubmed.ncbi.nlm.nih.gov/29744829

Antimicrobial peptide capsids of de novo design

Autor: Hasan Alkassem, Emiliana De Santis, Nicola Micale, Baptiste Lamarre, Maxim G. Ryadnov, Angelo Bella, Santanu Ray, Alexander R. Yon, Nilofar Faruqui, Jonathan R. Burns, Bart W. Hoogenboom, James E. Noble

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Nature Communications

The spread of bacterial resistance to antibiotics poses the need for antimicrobial discovery. With traditional search paradigms being exhausted, approaches that are altogether different from antibiotics may offer promising and creative solutions. Her

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30be435cbbd70991a5ac7707559aa4a6
https://doi.org/10.1038/s41467-017-02475-3