Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Emil M, Orozco"'
Publikováno v:
PeerJ, Vol 8, p e8362 (2020)
We present a new approach to edit both mitochondrial and chloroplast genomes. Organelles have been considered off-limits to CRISPR due to their impermeability to most RNA and DNA. This has prevented applications of Cas9/gRNA-mediated genome editing i
Externí odkaz:
https://doaj.org/article/38e954cafe404bd3b361e7c84f472e24
Publikováno v:
PeerJ, Vol 8, p e8362 (2020)
We present a new approach to edit both mitochondrial and chloroplast genomes. Organelles have been considered off-limits to CRISPR due to their impermeability to most RNA and DNA. This has prevented applications of Cas9/gRNA-mediated genome editing i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42aefdfe3ec505b7f4de6a38381aa239
https://doi.org/10.7717/peerj.8362
https://doi.org/10.7717/peerj.8362
Publikováno v:
PeerJ
We present a new approach to edit both mitochondrial and chloroplast genomes. Organelles have been considered off-limits to CRISPR due to their impermeability to most RNA and DNA. This has prevented applications of Cas9/gRNA-mediated genome editing i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::27596ee182d0ce041a8fa224705cd8cb
https://pubmed.ncbi.nlm.nih.gov/31934513
https://pubmed.ncbi.nlm.nih.gov/31934513
Autor:
Emil M. Orozco, Yuh-Jin Liang, Shih-Tong Jeng, Chi-Hui Lin, Liang-Jwu Chen, Ming-Te Yang, Richard I. Gumport
Publikováno v:
Nucleic Acids Research. 23:4690-4697
We have investigated the mechanism of transcription termination in vitro by spinach chloroplast RNA polymerase using templates encoding variants of the transcription-termination structure (attenuator) of the regulatory region of the threonine (thr) o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::692656039d7a07560f4a05ead3737174
https://doi.org/10.1093/nar/23.22.4690
https://doi.org/10.1093/nar/23.22.4690
Publikováno v:
Plant Molecular Biology. 15:421-435
Spinach chloroplast RNA polymerase has been shown to efficiently terminate transcription at the threonine attenuator (thra) from Escherichia coli [7]. In this study, efficient transcription termination by the chloroplast RNA polymerase was observed a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73fdbe25f52e4006c72f2caac96dd0b2
https://doi.org/10.1007/bf00019159
https://doi.org/10.1007/bf00019159
Publikováno v:
Current genetics. 17(1)
The in vivo transcripts of the tobacco chloroplast gene for the beta subunit of the ATPase (atpB) were examined. In tobacco, like spinach, the atpB gene encodes multiple transcripts. Six tobacco atpB transcripts are present in vivo, with 5' ends at p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::123d535ff4af6420b241fae4c0393f01
https://pubmed.ncbi.nlm.nih.gov/2138063
https://pubmed.ncbi.nlm.nih.gov/2138063
Publikováno v:
Nucleic Acids Research. 13:1283-1302
We have developed an homologous in vitro system from spinach chloroplasts that correctly initiates transcription of the plastid genes for the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) and the beta subunit of the plastid ATPase (at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71bd769c845a0472d58756c72074f13b
https://doi.org/10.1093/nar/13.4.1283
https://doi.org/10.1093/nar/13.4.1283
Publikováno v:
Molecular and Cellular Biology. 5:2733-2745
The large subunit gene (rbcL) of ribulose 1,5-bisphosphate carboxylase was transcribed in vitro by using maize and pea chloroplast extracts and a cloned plastid DNA template containing 172 base pairs (bp) of the maize rbcL protein-coding region and 7
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7fb08bc21f9a65779806c790ae56440
https://doi.org/10.1128/mcb.5.10.2733-2745.1985
https://doi.org/10.1128/mcb.5.10.2733-2745.1985
Purified chloroplast 5S, 16S, and 23S rRNAs and tRNAs were labeled in vitro and hybridized by the Southern method to ctDNA restriction fragments and cloned ctDNA fragments. Transfer RNA coding loci were found scattered throughout the genome, as well
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f267832b68134f1d2c82ada0277e9e44
https://doi.org/10.1016/b978-0-12-198780-0.50014-3
https://doi.org/10.1016/b978-0-12-198780-0.50014-3
Autor:
Liang-Jwu Chen, Emil M. Orozco
Publikováno v:
Nucleic acids research. 16(17)
To determine whether chloroplast RNA polymerase will accurately terminate transcription in vitro, we have fused the spinach chloroplast rbcL promoter to the 3' end of the rbcL gene as well as to various factor independent transcription terminators fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb995525a0002e36926a3276b114933b
https://pubmed.ncbi.nlm.nih.gov/2843817
https://pubmed.ncbi.nlm.nih.gov/2843817