Optimized Detection of Plasmodium falciparum Topoisomerase I Enzyme Activity in a Complex Biological Sample by the Use of Molecular Beacons

Autor: Asger Givskov, Emil L. Kristoffersen, Kamilla Vandsø, Yi-Ping Ho, Magnus Stougaard, Birgitta R. Knudsen

Publikováno v: Sensors, Vol 16, Iss 11, p 1916 (2016)

The so-called Rolling Circle Amplification allows for amplification of circular DNA structures in a manner that can be detected in real-time using nucleotide-based molecular beacons that unfold upon recognition of the DNA product, which is being prod

Externí odkaz: https://doaj.org/article/50bf8340515f4ef89e977759c0f0a315

Ultra-fast detection and quantification of nucleic acids by amplification-free fluorescence assay

Autor: Jonas Hansen, Ger J. M. Pruijn, Christian Damsgaard Jørgensen, Emil L. Kristoffersen, Christina Westmose Yde, Jesper Uhd, Hanlee P. Ji, Marina Dunaeva, Finn Cilius Nielsen, Victoria Birkedal, Kira Astakhova, Laura Miotke

Publikováno v: Uhd, J, Miotke, L, Ji, H P, Dunaeva, M, Pruijn, G J M, Jørgensen, C D, Kristoffersen, E L, Birkedal, V, Yde, C W, Nielsen, F C, Hansen, J & Astakhova, K 2020, ' Ultra-fast detection and quantification of nucleic acids by amplification-free fluorescence assay ', Analyst, vol. 145, no. 17, pp. 5836-5844 . https://doi.org/10.1039/d0an00676a
Uhd, J, Miotke, L, Ji, H P, Dunaeva, M, Pruijn, G J M, Jørgensen, C D, Kristoffersen, E L, Birkedal, V, Yde, C W, Nielsen, F C, Hansen, J & Astakhova, K 2020, ' Ultra-fast detection and quantification of nucleic acids by amplification-free fluorescence assay ', The Analyst, vol. 145, no. 17, pp. 5836-5844 . https://doi.org/10.1039/d0an00676a
Analyst, 145, 5836-5844
Analyst, 145, 17, pp. 5836-5844

Two types of clinically important nucleic acid biomarkers, microRNA (miRNA) and circulating tumor DNA (ctDNA) were detected and quantified from human serum using an amplification-free fluorescence hybridization assay. Specifically, miRNAs hsa-miR-223

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7dc8ea110a37b63ca061d4c6eced5c35
https://pubmed.ncbi.nlm.nih.gov/32648858

Topoisomerase I activity and sensitivity to camptothecin in breast cancer-derived cells: a comparative study

Autor: Ann Katrine Jakobsen, Irina Gromova, Birgitta R. Knudsen, Line Algreen, Anne Katrine W. Simonsen, Cinzia Tesauro, Kamilla Wandsoe Petersen, Marie Bech Andersen, Emil L. Kristoffersen, Pavel Gromov, Magnus Stougaard, Anne Bech Andersen, Noriko Yokoyama Hansen

Publikováno v: BMC Cancer
Tesauro, C, Simonsen, A K, Andersen, M B, Petersen, K W, Kristoffersen, E L, Algreen, L, Hansen, N Y, Andersen, A B, Jakobsen, A K, Stougaard, M, Gromov, P, Knudsen, B R & Gromova, I 2019, ' Topoisomerase I activity and sensitivity to camptothecin in breast cancer-derived cells : a comparative study ', BMC Cancer, vol. 19, no. 1, 1158 . https://doi.org/10.1186/s12885-019-6371-0
BMC Cancer, Vol 19, Iss 1, Pp 1-15 (2019)

BackgroundCamptothecin (CPT) and its derivatives are currently used as second- or third-line treatment for patients with endocrine-resistant breast cancer (BC). These drugs convert nuclear enzyme DNA topoisomerase I (TOP1) to a cell poison with the p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::193553046aae71ca9689e58dd225da3d
https://pubmed.ncbi.nlm.nih.gov/31783818

Real-time investigation of human topoisomerase I reaction kinetics using an optical sensor: a fast method for drug screening and determination of active enzyme concentrations

Autor: Lotte Bjergbaek, Magnus Stougaard, Line A. Jørgensen, Oskar Franch, Rikke Frøhlich, Michael Etzerodt, Birgitta R. Knudsen, Yi-Ping Ho, Emil L. Kristoffersen

Publikováno v: Kristoffersen, E L, Jørgensen, L A, Franch, O, Etzerodt, M, Frøhlich, R, Bjergbæk, L, Stougaard, M, Ho, Y-P & Knudsen, B R 2015, ' Real-time investigation of human topoisomerase I reaction kinetics using an optical sensor : a fast method for drug screening and determination of active enzyme concentrations ', Nanoscale, vol. 7, no. 21, pp. 9825-9834 . https://doi.org/10.1039/c5nr01474c

Human DNA topoisomerase I (hTopI) is a nuclear enzyme that catalyzes relaxation of super helical tension that arises in the genome during essential DNA metabolic processes. This is accomplished through a common reaction mechanism shared among the typ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1c85179b8c7e2f8aff1f08e4f4fe45e
https://doi.org/10.1039/c5nr01474c

Advantages of an optical nanosensor system for the mechanistic analysis of a novel topoisomerase I targeting drug:a case study

Autor: Rikke Frøhlich, Andrea Coletta, Yi-Ping Ho, María Jesús González González, Cinzia Tesauro, Emil L. Kristoffersen, Magnus Stougaard, Birgitta R. Knudsen, Francisco Palacios, Gloria Rubiales, Marie Bech Andersen, Concepción Alonso

Publikováno v: Andersen, M, Tesauro, C, Gonzalez, M, Kristoffersen, E L, Alonso, C, Rubiales, G, Coletta, A, Hougaard, R F, Stougaard, M, Ho, M Y-P, Palacios, F & Knudsen, B R 2017, ' Advantages of an optical nanosensor system for the mechanistic analysis of a novel topoisomerase I targeting drug : a case study ', Nanoscale, vol. 9, pp. 1886-1895 . https://doi.org/10.1039/C6NR06848K

The continuous need for the development of new small molecule anti-cancer drugs calls for easily accessible sensor systems for measuring the effect of vast numbers of new drugs on their potential cellular targets. Here we demonstrate the use of an op

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9db4dad9811eb76dc01c9df8892ec63e
https://pure.au.dk/portal/da/publications/advantages-of-an-optical-nanosensor-system-for-the-mechanistic-analysis-of-a-novel-topoisomerase-i-targeting-drug(3e505582-2c1c-4d99-ade0-55a8ccc87d2c).html