Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Emil J.H. Wolbert"'
Adaptation and application of a two-plasmid inducible CRISPR-Cas9 system in Clostridium beijerinckii
Autor:
Ana M. López-Contreras, John van der Oost, Marc W. T. Werten, Rémi Hocq, Servé W. M. Kengen, Mamou Diallo, Gwladys Chartier, François Wasels, Emil J.H. Wolbert, Nicolas Lopes Ferreira, Hani Surya Wijaya, Florent Collas
Publikováno v:
Methods
Methods, Elsevier, 2020, 172, pp.51-60. ⟨10.1016/j.ymeth.2019.07.022⟩
Methods 172 (2020)
Methods, 172, 51-60
Methods, Elsevier, 2020, 172, pp.51-60. ⟨10.1016/j.ymeth.2019.07.022⟩
Methods 172 (2020)
Methods, 172, 51-60
International audience; Recent developments in CRISPR technologies have opened new possibilities for improving genome editing tools dedicated to the Clostridium genus. In this study we adapted a two-plasmid tool based on this technology to enable sca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7afffa475c3fbeb075080eba149ae898
https://hal-ifp.archives-ouvertes.fr/hal-02572152
https://hal-ifp.archives-ouvertes.fr/hal-02572152
Autor:
Lenny de Jaeger, Gerrit Eggink, Camilo F. Muñoz, Ka Y.F. Lip, Emil J.H. Wolbert, René H. Wijffels, Dirk E. Martens, Justus W.J. Olijslager, Ruud A. Weusthuis, Mark H. J. Sturme, Jan Springer
Publikováno v:
Algal Research, 33, 448-455
Algal Research 33 (2018)
Algal Research 33 (2018)
Genetic transformation of microalgae remains a challenge due to poor intracellular delivery of exogenous molecules. This limitation is caused by the structure and composition of the cell wall and cell membrane of each species. Moreover, successful de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::771b00b5d2c702a75c91032168bd3a10
https://doi.org/10.1016/j.algal.2018.06.021
https://doi.org/10.1016/j.algal.2018.06.021
Autor:
L. de Jaeger, Emil J.H. Wolbert, Dirk E. Martens, René H. Wijffels, Jan Springer, Gerrit Eggink
Publikováno v:
Bioresource Technology
Bioresource Technology, 245(B), 1616-1626
Bioresource Technology 245 (2017) B
Bioresource Technology, 245(B), 1616-1626
Bioresource Technology 245 (2017) B
In this study, stearoyl-ACP desaturase (SAD), the enzyme that converts stearic acid into oleic acid, is silenced by artificial microRNA in the green microalga Chlamydomonas reinhardtii. Two different constructs, which target different positions on th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e43742cf6e299a5e4222b64563f167cc
https://hdl.handle.net/11250/2489894
https://hdl.handle.net/11250/2489894
Autor:
Ruud A. Weusthuis, Peter J. Schaap, G.Bwee Houweling-Tan, Marc W. T. Werten, Mark Levisson, Sjon Hendriks, Y. Griekspoor, Hetty van der Wal, Audrey Leprince, Emil J.H. Wolbert, Astrid E. Mars, Jan Springer, Truus de Vrije, John van der Oost, Gerrit Eggink, Antoine P. H. A. Moers, O. Mendes
Publikováno v:
Metabolic Engineering 42 (2017)
Metabolic Engineering, 42, 66-73
Metabolic Engineering, 42, 66-73
A Monascus ruber strain was isolated that was able to grow on mineral medium at high sugar concentrations and 175 g/l lactic acid at pH 2.8. Its genome and transcriptomes were sequenced and annotated. Genes encoding lactate dehydrogenase (LDH) were i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2a1a28cf60d5820588ef0483c83ff8a
https://research.wur.nl/en/publications/monascus-ruber-as-cell-factory-for-lactic-acid-production-at-low-
https://research.wur.nl/en/publications/monascus-ruber-as-cell-factory-for-lactic-acid-production-at-low-
Autor:
H. Zhang, L. de Jaeger, René B. Draaisma, Jan Springer, E.J. van den End, Maria J. Barbosa, V.A.P. Martins dos Santos, Dorinde M.M. Kleinegris, Guido Breuer, Gerrit Eggink, I. Schäffers, Peter J. Schaap, Packo P. Lamers, Dirk E. Martens, René H. Wijffels, Emil J.H. Wolbert, B.M. Carreres
Publikováno v:
Genome Announcements, 5(3)
Genome Announcements
Genome Announcements 5 (2017) 3
Genome Announcements
Genome Announcements 5 (2017) 3
The microalgae Tetradesmus obliquus is able to maintain a high photosynthetic efficiency under nitrogen limitation and is considered a promising green microalgae for sustainable production of diverse compounds, including biofuels. Here, we report the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3ad2be83941b7259c09041fe872b27c
https://doi.org/10.1128/genomea.01449-16
https://doi.org/10.1128/genomea.01449-16
Publikováno v:
Journal of Biotechnology 146 (2010) 1-2
Journal of Biotechnology, 146(1-2), 66-73
Journal of Biotechnology, 146(1-2), 66-73
The undecapeptides CH(3)CO-Gln-Gln-Arg-Phe-Gln-Trp-Gln-Phe-Glu-Gln-Gln-NH(2) (P(11)-2) and CH(3)CO-Gln-Gln-Orn-Phe-Orn-Trp-Orn-Phe-Orn-Gln-Gln-NH(2) (P(11)-14) have unique self-assembly characteristics and broad application potential. Originally, the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0ddef8fc3fd04eb9f8ed87930c7dbba
https://doi.org/10.1016/j.jbiotec.2010.01.010
https://doi.org/10.1016/j.jbiotec.2010.01.010
Publikováno v:
Plant Molecular Biology. 33:989-999
The gene encoding green fluorescent protein (GFP) from Aequorea victoria was resynthesized to adapt its codon usage for expression in plants by increasing the frequency of codons with a C or a G in the third position from 32 to 60%. The strategy for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5f9be8c5830a5db3db0be92f12ba4dc4
https://doi.org/10.1023/a:1005740823703
https://doi.org/10.1023/a:1005740823703
Autor:
Emil J.H. Wolbert, Gerrit Eggink, Helena Teles, Antoine P. H. A. Moers, Marc W. T. Werten, Frits A. de Wolf, Joris Sprakel
Publikováno v:
Biomacromolecules, 10(5), 1106-1113
Biomacromolecules 10 (2009) 5
Biomacromolecules 10 (2009) 5
Gelatin hydrogels find broad medical application. The current materials, however, are from animal sources, and their molecular structure and thermal properties cannot be controlled. This study describes recombinant gelatin-like polymers with a genera
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::156f18f038b54d90e35bd12c31c54a9c
https://research.wur.nl/en/publications/precision-gels-from-collagen-inspired-triblock-copolymers
https://research.wur.nl/en/publications/precision-gels-from-collagen-inspired-triblock-copolymers
Autor:
Jan Springer, Lute-Harm Zwiers, Henk J. Huizing, Emil J.H. Wolbert, Hans Mooibroek, Gerard J. A. Rouwendal
Publikováno v:
Gene, 171, 59-63
Gene 171 (1996)
Gene 171 (1996)
A chromosomal DNA fragment containing the Bacillus macquariensis (Bm) ATP-dependent phosphofructokinase-encoding gene (pfk) was cloned from a subgenomic library in pUC19 using a PCR-derived probe. The region containing pfk, including flanking sequenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c83f616ae5eb6152d73a43376e85cd5f
https://research.wur.nl/en/publications/cloning-sequence-and-expression-in-escherichia-coli-of-the-gene-e
https://research.wur.nl/en/publications/cloning-sequence-and-expression-in-escherichia-coli-of-the-gene-e